Efficient removal of lignin with the maintenance of hemicellulose from kenaf by two-stage pretreatment process

Nur Izyan Wan Azelee, Jamaliah Md Jahim, Amir Rabu, Abdul Munir Abd. Murad, Farah Diba Abu Bakar, Rosli Md Illias

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The enhancement of lignocellulose hydrolysis using enzyme complexes requires an efficient pretreatment process to obtain susceptible conditions for the enzyme attack. This study focuses on removing a major part of the lignin layer from kenaf (Hibiscus cannabinus) while simultaneously maintaining most of the hemicellulose. A two-stage pretreatment process is adopted using calcium hydroxide, Ca(OH)2, and peracetic acid, PPA, to break the recalcitrant lignin layer from other structural polysaccharides. An experimental screening of several pretreatment chemicals, concentrations, temperatures and solid-liquid ratios enabled the production of an optimally designed pretreatment process for kenaf. Our results showed that the pretreatment process has provide 59.25% lignin removal while maintaining 87.72% and 96.17% hemicellulose and cellulose, respectively, using 1 g of Ca(OH)2/L and a 8:1 (mL:g) ratio of liquid-Ca(OH)2 at 50 C for 1.5 h followed by 20% peracetic acid pretreatment at 75 C for 2 h. These results validate this mild approach for aiding future enzymatic hydrolysis.

Original languageEnglish
Pages (from-to)447-453
Number of pages7
JournalCarbohydrate Polymers
Volume99
DOIs
Publication statusPublished - 2014

Fingerprint

Lignin
Peracetic Acid
Enzymes
Calcium Hydroxide
Hydrated lime
Enzymatic hydrolysis
Acids
Liquids
Polysaccharides
Cellulose
Hydrolysis
Screening
hemicellulose
Temperature

Keywords

  • Hemicellulose
  • Kenaf
  • Lignin removal
  • Pretreatment

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Efficient removal of lignin with the maintenance of hemicellulose from kenaf by two-stage pretreatment process. / Wan Azelee, Nur Izyan; Md Jahim, Jamaliah; Rabu, Amir; Abd. Murad, Abdul Munir; Abu Bakar, Farah Diba; Md Illias, Rosli.

In: Carbohydrate Polymers, Vol. 99, 2014, p. 447-453.

Research output: Contribution to journalArticle

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