Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) through somatic embryogenesis

Z. A. Rahman, A. Ramli, H. Hosni, R. Kamaruzaman, Z. A. Seman, A. N. Othman, Zamri Zainal, J. Uddain, S. Subramaniam

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aromatic rice variety namely MRQ 74, MRQ 80 and MRQ 50 are the most popular rice in Malaysia. Establishing an efficient regeneration protocols via somatic embryogenesis is required for varietal improvements. An efficient somatic embryogenesis system has been established for three aromatic rice varieties using mature seeds. MS-B5 medium containing 1 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 10 mg/L NAA (a-naphthaleneacetic acid) optimized that produced highest embryogenic callus (89%) without any browning effect. The highest whitish somatic embryos frequency (75%) was initiated by incubating embryogenic calli on same medium containing 10 mg/L ABA and 9 g/L Gelrite for 8 weeks. However, the numbers of regenerated plantlets on medium containing NAA and 2,4-D which was previously pre-treated with 10 mg/L ABA, 9 g/L Gelrite and incubation at 8 weeks was the best method for shoots induction of three rice varieties.

Original languageEnglish
Pages (from-to)857-863
Number of pages7
JournalEmirates Journal of Food and Agriculture
Volume27
Issue number11
Publication statusPublished - 2015

Fingerprint

somatic embryogenesis
2,4-Dichlorophenoxyacetic Acid
Embryonic Development
aromatic compounds
Regeneration
Oryza sativa
Naphthaleneacetic Acids
2,4-D
rice
Bony Callus
naphthaleneacetic acid
callus
Malaysia
somatic embryos
plantlets
Seeds
Embryonic Structures
shoots
Oryza
seeds

Keywords

  • Aromatic rice
  • Carbon sources
  • Plant growth regulators
  • Somatic embryogenesis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Animal Science and Zoology
  • Food Science
  • Applied Microbiology and Biotechnology

Cite this

Rahman, Z. A., Ramli, A., Hosni, H., Kamaruzaman, R., Seman, Z. A., Othman, A. N., ... Subramaniam, S. (2015). Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) through somatic embryogenesis. Emirates Journal of Food and Agriculture, 27(11), 857-863.

Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) through somatic embryogenesis. / Rahman, Z. A.; Ramli, A.; Hosni, H.; Kamaruzaman, R.; Seman, Z. A.; Othman, A. N.; Zainal, Zamri; Uddain, J.; Subramaniam, S.

In: Emirates Journal of Food and Agriculture, Vol. 27, No. 11, 2015, p. 857-863.

Research output: Contribution to journalArticle

Rahman, ZA, Ramli, A, Hosni, H, Kamaruzaman, R, Seman, ZA, Othman, AN, Zainal, Z, Uddain, J & Subramaniam, S 2015, 'Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) through somatic embryogenesis', Emirates Journal of Food and Agriculture, vol. 27, no. 11, pp. 857-863.
Rahman, Z. A. ; Ramli, A. ; Hosni, H. ; Kamaruzaman, R. ; Seman, Z. A. ; Othman, A. N. ; Zainal, Zamri ; Uddain, J. ; Subramaniam, S. / Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) through somatic embryogenesis. In: Emirates Journal of Food and Agriculture. 2015 ; Vol. 27, No. 11. pp. 857-863.
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