Efficacy of hypocrellin pharmacokinetics in phototherapy.

Seyed Mohamed Ali, Malini Olivo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Hypocrellins A and B are pigments which are isolated from the parasitic fungi Hypocrella bambuase sacc and Shiraia bambusicola P. Heen found in the People's Republic of China and other parts of Asia including Sri Lanka. These agents, which belong to the general class of perylene quinonoid pigments, have a long history of traditional medicinal agents especially in Asia. Hypocrellins are under extensive investigation as photosensitizing agents for photodynamic therapy (PDT). Hypocrellin compounds were selected as potential photosensitizers for PDT owing to their high quantum yields of singlet oxygen (1O2), and facility for site-directed chemical modification to enhance phototoxicity, pharmacokinetics, solubility, and light absorption in the red spectral region, among other properties. The cellular uptake, evaluated by spectrofluorimetry and confocal laser scanning microscopy (CLSM) demonstrated that both HA and HB exhibited high and fast uptake and rapid internalization as revealed by their bio-distribution pattern. In addition, the present study employed both immunocytochemical and Western blot techniques to explore the photo-induced expression of apoptosis related proteins in NPC as well as other human carcinoma cells. Using spectrofluorimetry and CLSM we have determined the cellular fluorescence as a marker for the uptake of HA and HB. Co-staining with either HA or HB and fluorescent dyes specific for cell organelles revealed an intracellular localization of HA and HB in lysosomes other than mitochondria.

Original languageEnglish
Pages (from-to)1229-1237
Number of pages9
JournalInternational Journal of Oncology
Volume21
Issue number6
Publication statusPublished - Dec 2002
Externally publishedYes

Fingerprint

Phototherapy
Photosensitizing Agents
Pharmacokinetics
Photochemotherapy
Confocal Microscopy
Perylene
Phototoxic Dermatitis
Sri Lanka
Singlet Oxygen
Lysosomes
Fluorescent Dyes
Organelles
Solubility
China
Mitochondria
Fungi
Fluorescence
Western Blotting
Apoptosis
Staining and Labeling

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Efficacy of hypocrellin pharmacokinetics in phototherapy. / Ali, Seyed Mohamed; Olivo, Malini.

In: International Journal of Oncology, Vol. 21, No. 6, 12.2002, p. 1229-1237.

Research output: Contribution to journalArticle

Ali, SM & Olivo, M 2002, 'Efficacy of hypocrellin pharmacokinetics in phototherapy.', International Journal of Oncology, vol. 21, no. 6, pp. 1229-1237.
Ali, Seyed Mohamed ; Olivo, Malini. / Efficacy of hypocrellin pharmacokinetics in phototherapy. In: International Journal of Oncology. 2002 ; Vol. 21, No. 6. pp. 1229-1237.
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