Efficacy of Human Cell-Seeded Muscle-Stuffed Vein Conduit in Rat Sciatic Nerve Repair

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1 Citation (Scopus)

Abstract

We investigated the efficacy of a muscle-stuffed vein (MSV) seeded with neural-transdifferentiated human mesenchymal stem cells as an alternative nerve conduit to repair a 15-mm sciatic nerve defect in athymic rats. Other rats received MSV conduit alone, commercial polyglycolic acid conduit (Neurotube®), reverse autograft, or were left untreated. Motor and sensory functions as well as nerve conductivity were evaluated for 12 weeks, after which the grafts were harvested for histological analyses. All rats in the treatment groups demonstrated a progressive increase in the mean Sciatic Functional Index (motor function) and nerve conduction amplitude (electrophysiological function) and showed positive withdrawal reflex (sensory function) by the 10th week of postimplantation. Autotomy, which is associated with neuropathic pain, was severe in rats treated with conduit without cells; there was mild or no autotomy in the rats of other groups. Histologically, harvested grafts from all except the untreated groups exhibited axonal regeneration with the presence of mature myelinated axons. In conclusion, treatment with MSV conduit is comparable to that of other treatment groups in supporting functional recovery following sciatic nerve injury; and the addition of cells in the conduit alleviates neuropathic pain. It is shown that pretreated muscle-stuffed vein conduit is comparable to that of commercial nerve conduit and autograft in supporting functional recovery following peripheral nerve injury. The addition of neural-differentiated mesenchymal stem cells in the conduit is shown to alleviate neuropathic pain.

Original languageEnglish
Pages (from-to)1438-1455
Number of pages18
JournalTissue Engineering - Part A
Volume25
Issue number19-20
DOIs
Publication statusPublished - Oct 2019

Fingerprint

Sciatic Nerve
Muscle Cells
Muscle
Rats
Veins
Neuralgia
Repair
Cells
Muscles
Autografts
Mesenchymal Stromal Cells
Stem cells
Grafts
Nude Rats
Polyglycolic Acid
Transplants
Peripheral Nerve Injuries
Neural Conduction
Recovery
Axons

Keywords

  • autotomy
  • bone marrow-derived mesenchymal stem cell
  • conduit patency
  • neuroma
  • Schwann cell-like cells
  • tissue-engineered nerve guidance conduit

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

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title = "Efficacy of Human Cell-Seeded Muscle-Stuffed Vein Conduit in Rat Sciatic Nerve Repair",
abstract = "We investigated the efficacy of a muscle-stuffed vein (MSV) seeded with neural-transdifferentiated human mesenchymal stem cells as an alternative nerve conduit to repair a 15-mm sciatic nerve defect in athymic rats. Other rats received MSV conduit alone, commercial polyglycolic acid conduit (Neurotube{\circledR}), reverse autograft, or were left untreated. Motor and sensory functions as well as nerve conductivity were evaluated for 12 weeks, after which the grafts were harvested for histological analyses. All rats in the treatment groups demonstrated a progressive increase in the mean Sciatic Functional Index (motor function) and nerve conduction amplitude (electrophysiological function) and showed positive withdrawal reflex (sensory function) by the 10th week of postimplantation. Autotomy, which is associated with neuropathic pain, was severe in rats treated with conduit without cells; there was mild or no autotomy in the rats of other groups. Histologically, harvested grafts from all except the untreated groups exhibited axonal regeneration with the presence of mature myelinated axons. In conclusion, treatment with MSV conduit is comparable to that of other treatment groups in supporting functional recovery following sciatic nerve injury; and the addition of cells in the conduit alleviates neuropathic pain. It is shown that pretreated muscle-stuffed vein conduit is comparable to that of commercial nerve conduit and autograft in supporting functional recovery following peripheral nerve injury. The addition of neural-differentiated mesenchymal stem cells in the conduit is shown to alleviate neuropathic pain.",
keywords = "autotomy, bone marrow-derived mesenchymal stem cell, conduit patency, neuroma, Schwann cell-like cells, tissue-engineered nerve guidance conduit",
author = "Khairunnisa Ramli and Gasim, {Aminath Ifasha} and Ahmad, {Amir Adham} and Ohnmar Htwe and {Mohamed Haflah}, {Nor Hazla} and Law, {Zhe Kang} and Shariful Hasan and Naicker, {Amaramalar Selvi} and Mokhtar, {Sabarul Afian} and {Muhamad Ariffin}, {Mohd Hisam} and Azmi Baharudin and Tan, {Geok Chin} and {Haji Idrus}, Ruszymah and Shalimar Abdullah and Ng, {Min Hwei}",
year = "2019",
month = "10",
doi = "10.1089/ten.tea.2018.0279",
language = "English",
volume = "25",
pages = "1438--1455",
journal = "Tissue Engineering - Part A.",
issn = "1937-3341",
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TY - JOUR

T1 - Efficacy of Human Cell-Seeded Muscle-Stuffed Vein Conduit in Rat Sciatic Nerve Repair

AU - Ramli, Khairunnisa

AU - Gasim, Aminath Ifasha

AU - Ahmad, Amir Adham

AU - Htwe, Ohnmar

AU - Mohamed Haflah, Nor Hazla

AU - Law, Zhe Kang

AU - Hasan, Shariful

AU - Naicker, Amaramalar Selvi

AU - Mokhtar, Sabarul Afian

AU - Muhamad Ariffin, Mohd Hisam

AU - Baharudin, Azmi

AU - Tan, Geok Chin

AU - Haji Idrus, Ruszymah

AU - Abdullah, Shalimar

AU - Ng, Min Hwei

PY - 2019/10

Y1 - 2019/10

N2 - We investigated the efficacy of a muscle-stuffed vein (MSV) seeded with neural-transdifferentiated human mesenchymal stem cells as an alternative nerve conduit to repair a 15-mm sciatic nerve defect in athymic rats. Other rats received MSV conduit alone, commercial polyglycolic acid conduit (Neurotube®), reverse autograft, or were left untreated. Motor and sensory functions as well as nerve conductivity were evaluated for 12 weeks, after which the grafts were harvested for histological analyses. All rats in the treatment groups demonstrated a progressive increase in the mean Sciatic Functional Index (motor function) and nerve conduction amplitude (electrophysiological function) and showed positive withdrawal reflex (sensory function) by the 10th week of postimplantation. Autotomy, which is associated with neuropathic pain, was severe in rats treated with conduit without cells; there was mild or no autotomy in the rats of other groups. Histologically, harvested grafts from all except the untreated groups exhibited axonal regeneration with the presence of mature myelinated axons. In conclusion, treatment with MSV conduit is comparable to that of other treatment groups in supporting functional recovery following sciatic nerve injury; and the addition of cells in the conduit alleviates neuropathic pain. It is shown that pretreated muscle-stuffed vein conduit is comparable to that of commercial nerve conduit and autograft in supporting functional recovery following peripheral nerve injury. The addition of neural-differentiated mesenchymal stem cells in the conduit is shown to alleviate neuropathic pain.

AB - We investigated the efficacy of a muscle-stuffed vein (MSV) seeded with neural-transdifferentiated human mesenchymal stem cells as an alternative nerve conduit to repair a 15-mm sciatic nerve defect in athymic rats. Other rats received MSV conduit alone, commercial polyglycolic acid conduit (Neurotube®), reverse autograft, or were left untreated. Motor and sensory functions as well as nerve conductivity were evaluated for 12 weeks, after which the grafts were harvested for histological analyses. All rats in the treatment groups demonstrated a progressive increase in the mean Sciatic Functional Index (motor function) and nerve conduction amplitude (electrophysiological function) and showed positive withdrawal reflex (sensory function) by the 10th week of postimplantation. Autotomy, which is associated with neuropathic pain, was severe in rats treated with conduit without cells; there was mild or no autotomy in the rats of other groups. Histologically, harvested grafts from all except the untreated groups exhibited axonal regeneration with the presence of mature myelinated axons. In conclusion, treatment with MSV conduit is comparable to that of other treatment groups in supporting functional recovery following sciatic nerve injury; and the addition of cells in the conduit alleviates neuropathic pain. It is shown that pretreated muscle-stuffed vein conduit is comparable to that of commercial nerve conduit and autograft in supporting functional recovery following peripheral nerve injury. The addition of neural-differentiated mesenchymal stem cells in the conduit is shown to alleviate neuropathic pain.

KW - autotomy

KW - bone marrow-derived mesenchymal stem cell

KW - conduit patency

KW - neuroma

KW - Schwann cell-like cells

KW - tissue-engineered nerve guidance conduit

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U2 - 10.1089/ten.tea.2018.0279

DO - 10.1089/ten.tea.2018.0279

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JO - Tissue Engineering - Part A.

JF - Tissue Engineering - Part A.

SN - 1937-3341

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