Effects of vitamin E on serum leptin levels and fat mass in intact and ovariectomized female rats

Research output: Contribution to journalArticle

Abstract

The aim of this study was to determine the effects of vitamin E deficiency and supplementation on serum leptin and fat mass in intact and ovariectomized female rats. One hundred and twenty 3-month old Wistar rats were used, where half were ovariectomized (OVX) and the other half were left intact. The intact and OVX rats were farther divided into 6 groups i.e. vitamin E deficient diets (VED, 75%VED, 50%VED, 25%VED), rat chow diet (RC) and rat chow with oral supplementation of 30 mg/kg body weight of α-tocopherol (vitamin E) (RC+ATF). After 15 weeks of treatment, fat mass of intact rats measured using "Dual Energy X-ray Absorptiometry" technique (DEXA) were increased only in the RC and RC+ATF groups. Leptin levels in intact rats was also significantly higher only in the RC and RC+ATF groups. In OVX rats, fat mass and leptin levels were increased in the VED, 50%VED, RC and RC+ATF groups. However, the significance of the increase in fat mass and leptin levels in the RC and RC+ATF groups of OVX rats was more apparent than that of intact rats. The less significant p values in OVX rats given VED diets indicated that in estrogen deficiency states, vitamin E deficiency inhibited further increase in fat mass and leptin levels seen in rats given adequate vitamin E. Fat mass was found to be positively correlated with leptin levels before and after treatment suggesting the development of leptin resistance. In conclusion, high levels of dietary vitamin E was associated with increased body fat mass while dietary deficiency of vitamin E reduced body fat mass. Large body fat mass was positively correlated with serum leptin levels suggesting that vitamin E may play a role in the development of leptin resistance.

Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalAsia Pacific Journal of Pharmacology
Volume16
Issue number3-4
Publication statusPublished - Dec 2006

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Leptin
Vitamin E
Fats
Serum
Diet
Vitamin E Deficiency
Adipose Tissue
Tocopherols
Photon Absorptiometry

Keywords

  • Fat mass
  • Female rats
  • Leptin
  • Ovariectomy
  • Vitamin E deficiency

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of vitamin E on serum leptin levels and fat mass in intact and ovariectomized female rats. / Abdullah, Azman; Khalid, B. A K; Soelaiman, Ima Nirwana.

In: Asia Pacific Journal of Pharmacology, Vol. 16, No. 3-4, 12.2006, p. 131-139.

Research output: Contribution to journalArticle

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