Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture

Ling L. Chia, Ibrahim Jantan, Chua Kien Hui, Kok Wai Lam, Kamal Rullah, Mohd F M Aluwi

Research output: Contribution to journalArticle

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Abstract

Tocotrienols (T3) are well-known for their antioxidant properties besides showing therapeutic potential in clinical complications such as hyperlipidemia induced by diabetes. The aim of this study was to determine the effects of δ-T3, γ-T3, and α-T3 on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. Pancreatic islets freshly isolated from male Wistar rats were treated with T3 for 1 h at 37°C in a microfluidic system with continuous operation. The cells were collected for total RNA extraction and reverse-transcribed, followed by measurement of insulin secretion-associated genes expression using quantitative real-time polymerase chain reaction. Molecular docking experiments were performed to gain insights on how the T3 bind to the receptors. Short-term exposure of δ- and γ-T3 to pancreatic β cells in a stimulant glucose condition (16.7 mM) significantly regulated preproinsulin mRNA levels and insulin gene transcription. In contrast, α-T3 possessed less ability in the activation of insulin synthesis level. Essentially, potassium chloride (KCl), a β cell membrane depolarising agent added into the treatment further enhanced the insulin production. δ- and γ-T3 revealed significantly higher quantitative expression in most of the insulin secretion-associated genes groups containing 16.7 mM glucose alone and 16.7 mM glucose with 30 mM KCl ranging from 600 to 1200 μM (p < 0.05). The findings suggest the potential of δ-T3 in regulating insulin synthesis and glucose-stimulated insulin secretion through triggering pathway especially in the presence of KCl.

Original languageEnglish
Article number291
JournalFrontiers in Pharmacology
Volume7
Issue numberAUG
DOIs
Publication statusPublished - 30 Aug 2016

Fingerprint

Tocotrienols
Islets of Langerhans
Insulin
Gene Expression
Glucose
Microfluidics
Potassium Chloride
Hyperlipidemias
Genes
Wistar Rats
Real-Time Polymerase Chain Reaction
Antioxidants
Cell Membrane
RNA
Messenger RNA

Keywords

  • Gene expression profiling
  • Insulin
  • Microfluidic system
  • Molecular docking
  • QRT-PCR
  • Tocotrienols

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. / Chia, Ling L.; Jantan, Ibrahim; Kien Hui, Chua; Lam, Kok Wai; Rullah, Kamal; Aluwi, Mohd F M.

In: Frontiers in Pharmacology, Vol. 7, No. AUG, 291, 30.08.2016.

Research output: Contribution to journalArticle

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