Pengaruh suhu terhadap perkembangan lalat berkepentingan forensik chrysomya villeneuvi patton (Diptera

Calliphoridae)

Translated title of the contribution: Effects of temperatures on development of a forensically important fly chrysomya villeneuvi patton (Diptera: Calliphoridae )

Ahmad Firdaus Mohd Salleh, Mohamed Abdullah Marwi, Syamsa Rizal Abdullah, Aishah Hani Azil, Baharudin Omar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Forensic entomology is a field of knowledge that uses insects as evidence to estimate the duration after death or post mortem interval (PMI). The PMI determination is based on the developmental size and stage of the insects found on the dead body. Chrysomya villeneuvi is one of the important species of flies that can serve as an indicator for estimating the PMI. This study aimed to develop a database on the developmental progress of Ch. villeneuvi at different temperatures. The data are also used to develop accumulated degree hours (ADH) tables. Justification of this study is because the developmental data of Ch. villeneuvi have not been developed in Malaysia. PMI determinations were made by referring to Ch. megacephala developmental data. This will cause calculation of PMI becomes less precise. Earlier studies also involve a relating morphology and behavior of this species. Observations on the development of eggs, larvae and pupae of Ch. villeneuvi were carried out at 25,27C, 30, 33 and 37°C inside an insect growth chamber. Adult flies were obtained by placing dead rats at the University of Malaya Research Center, 16th Mile, Gombak. These adult flies were reared in the laboratory as a source for the colony. Approximately 150 eggs were placed in a container with 200 g of beef liver as a food source for newly hatched larvae. The lengths of five larvae were measured every 3 h to get an average value for size at each temperature. Photographs and slides were also processed to ascertain the larval development. Ch. villeneuvi needed 9:40 ± 0.02 days at 25°C, 9:34 ± 0.04 days (27°C), 9.00 ± 0.07 days (30°C), 7.95 ± 0.02 days (33°C) and 7:51 ± 0.02 days (37°C) to complete its life cycle. There is a significant difference (p<0.001 ) on the duration of the life cycle of Ch. villeneuvi at different temperatures. The higher the temperature and the ADH, the shorter of the life cycle of flies Ch. villeneuvi. Knowledge about the duration of stages in a temperature-dependent life cycle facilitates the development of ADH data. Forensic investigators in Malaysia can calculate PMI based on developmental graft and ADH data obtained from this study when Ch. villeneuvi is found on corpses.

Original languageUndefined/Unknown
Pages (from-to)1537-1542
Number of pages6
JournalSains Malaysiana
Volume43
Issue number10
Publication statusPublished - 1 Oct 2014

Fingerprint

Chrysomya
Calliphoridae
life cycle (organisms)
livers as food
temperature
Malaysia
duration
larvae
forensic entomology
Malaya
insect growth
insects
growth chambers
larval development
photographs
pupae
containers
beef
death
rats

Keywords

  • Accumulated degree hour (ADH)
  • Chrysomya villeneuvi
  • Post-mortem interval
  • Temperature

ASJC Scopus subject areas

  • General

Cite this

Pengaruh suhu terhadap perkembangan lalat berkepentingan forensik chrysomya villeneuvi patton (Diptera : Calliphoridae). / Salleh, Ahmad Firdaus Mohd; Marwi, Mohamed Abdullah; Abdullah, Syamsa Rizal; Azil, Aishah Hani; Omar, Baharudin.

In: Sains Malaysiana, Vol. 43, No. 10, 01.10.2014, p. 1537-1542.

Research output: Contribution to journalArticle

@article{cf9781fc512e4e589cfe867bc94848d9,
title = "Pengaruh suhu terhadap perkembangan lalat berkepentingan forensik chrysomya villeneuvi patton (Diptera: Calliphoridae)",
abstract = "Forensic entomology is a field of knowledge that uses insects as evidence to estimate the duration after death or post mortem interval (PMI). The PMI determination is based on the developmental size and stage of the insects found on the dead body. Chrysomya villeneuvi is one of the important species of flies that can serve as an indicator for estimating the PMI. This study aimed to develop a database on the developmental progress of Ch. villeneuvi at different temperatures. The data are also used to develop accumulated degree hours (ADH) tables. Justification of this study is because the developmental data of Ch. villeneuvi have not been developed in Malaysia. PMI determinations were made by referring to Ch. megacephala developmental data. This will cause calculation of PMI becomes less precise. Earlier studies also involve a relating morphology and behavior of this species. Observations on the development of eggs, larvae and pupae of Ch. villeneuvi were carried out at 25,27C, 30, 33 and 37°C inside an insect growth chamber. Adult flies were obtained by placing dead rats at the University of Malaya Research Center, 16th Mile, Gombak. These adult flies were reared in the laboratory as a source for the colony. Approximately 150 eggs were placed in a container with 200 g of beef liver as a food source for newly hatched larvae. The lengths of five larvae were measured every 3 h to get an average value for size at each temperature. Photographs and slides were also processed to ascertain the larval development. Ch. villeneuvi needed 9:40 ± 0.02 days at 25°C, 9:34 ± 0.04 days (27°C), 9.00 ± 0.07 days (30°C), 7.95 ± 0.02 days (33°C) and 7:51 ± 0.02 days (37°C) to complete its life cycle. There is a significant difference (p<0.001 ) on the duration of the life cycle of Ch. villeneuvi at different temperatures. The higher the temperature and the ADH, the shorter of the life cycle of flies Ch. villeneuvi. Knowledge about the duration of stages in a temperature-dependent life cycle facilitates the development of ADH data. Forensic investigators in Malaysia can calculate PMI based on developmental graft and ADH data obtained from this study when Ch. villeneuvi is found on corpses.",
keywords = "Accumulated degree hour (ADH), Chrysomya villeneuvi, Post-mortem interval, Temperature",
author = "Salleh, {Ahmad Firdaus Mohd} and Marwi, {Mohamed Abdullah} and Abdullah, {Syamsa Rizal} and Azil, {Aishah Hani} and Baharudin Omar",
year = "2014",
month = "10",
day = "1",
language = "Undefined/Unknown",
volume = "43",
pages = "1537--1542",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "10",

}

TY - JOUR

T1 - Pengaruh suhu terhadap perkembangan lalat berkepentingan forensik chrysomya villeneuvi patton (Diptera

T2 - Calliphoridae)

AU - Salleh, Ahmad Firdaus Mohd

AU - Marwi, Mohamed Abdullah

AU - Abdullah, Syamsa Rizal

AU - Azil, Aishah Hani

AU - Omar, Baharudin

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Forensic entomology is a field of knowledge that uses insects as evidence to estimate the duration after death or post mortem interval (PMI). The PMI determination is based on the developmental size and stage of the insects found on the dead body. Chrysomya villeneuvi is one of the important species of flies that can serve as an indicator for estimating the PMI. This study aimed to develop a database on the developmental progress of Ch. villeneuvi at different temperatures. The data are also used to develop accumulated degree hours (ADH) tables. Justification of this study is because the developmental data of Ch. villeneuvi have not been developed in Malaysia. PMI determinations were made by referring to Ch. megacephala developmental data. This will cause calculation of PMI becomes less precise. Earlier studies also involve a relating morphology and behavior of this species. Observations on the development of eggs, larvae and pupae of Ch. villeneuvi were carried out at 25,27C, 30, 33 and 37°C inside an insect growth chamber. Adult flies were obtained by placing dead rats at the University of Malaya Research Center, 16th Mile, Gombak. These adult flies were reared in the laboratory as a source for the colony. Approximately 150 eggs were placed in a container with 200 g of beef liver as a food source for newly hatched larvae. The lengths of five larvae were measured every 3 h to get an average value for size at each temperature. Photographs and slides were also processed to ascertain the larval development. Ch. villeneuvi needed 9:40 ± 0.02 days at 25°C, 9:34 ± 0.04 days (27°C), 9.00 ± 0.07 days (30°C), 7.95 ± 0.02 days (33°C) and 7:51 ± 0.02 days (37°C) to complete its life cycle. There is a significant difference (p<0.001 ) on the duration of the life cycle of Ch. villeneuvi at different temperatures. The higher the temperature and the ADH, the shorter of the life cycle of flies Ch. villeneuvi. Knowledge about the duration of stages in a temperature-dependent life cycle facilitates the development of ADH data. Forensic investigators in Malaysia can calculate PMI based on developmental graft and ADH data obtained from this study when Ch. villeneuvi is found on corpses.

AB - Forensic entomology is a field of knowledge that uses insects as evidence to estimate the duration after death or post mortem interval (PMI). The PMI determination is based on the developmental size and stage of the insects found on the dead body. Chrysomya villeneuvi is one of the important species of flies that can serve as an indicator for estimating the PMI. This study aimed to develop a database on the developmental progress of Ch. villeneuvi at different temperatures. The data are also used to develop accumulated degree hours (ADH) tables. Justification of this study is because the developmental data of Ch. villeneuvi have not been developed in Malaysia. PMI determinations were made by referring to Ch. megacephala developmental data. This will cause calculation of PMI becomes less precise. Earlier studies also involve a relating morphology and behavior of this species. Observations on the development of eggs, larvae and pupae of Ch. villeneuvi were carried out at 25,27C, 30, 33 and 37°C inside an insect growth chamber. Adult flies were obtained by placing dead rats at the University of Malaya Research Center, 16th Mile, Gombak. These adult flies were reared in the laboratory as a source for the colony. Approximately 150 eggs were placed in a container with 200 g of beef liver as a food source for newly hatched larvae. The lengths of five larvae were measured every 3 h to get an average value for size at each temperature. Photographs and slides were also processed to ascertain the larval development. Ch. villeneuvi needed 9:40 ± 0.02 days at 25°C, 9:34 ± 0.04 days (27°C), 9.00 ± 0.07 days (30°C), 7.95 ± 0.02 days (33°C) and 7:51 ± 0.02 days (37°C) to complete its life cycle. There is a significant difference (p<0.001 ) on the duration of the life cycle of Ch. villeneuvi at different temperatures. The higher the temperature and the ADH, the shorter of the life cycle of flies Ch. villeneuvi. Knowledge about the duration of stages in a temperature-dependent life cycle facilitates the development of ADH data. Forensic investigators in Malaysia can calculate PMI based on developmental graft and ADH data obtained from this study when Ch. villeneuvi is found on corpses.

KW - Accumulated degree hour (ADH)

KW - Chrysomya villeneuvi

KW - Post-mortem interval

KW - Temperature

UR - http://www.scopus.com/inward/record.url?scp=84908702914&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908702914&partnerID=8YFLogxK

M3 - Article

VL - 43

SP - 1537

EP - 1542

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 10

ER -