Effects of solvents and extraction methods on herbal plants Phyllanthus niruri, Orthosiphon stamineus and Labisia pumila

Nur Aqilah Kamarudin, Masturah Markom, Jalifah Latip

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Objectives: Phyllanthus niruri, Orthosiphon stamineus and Labisia pumila are the three herbs listed in Malaysian National Key Economic Areas (NKEA). This study was conducted to determine the herbs extract yields and activities on antioxidant and cytotoxicity properties. Methods/Statistical Analysis: Cold maceration and soxhlet were the extraction methods employed and water, ethanol and 50% ethanol (v/v) were chosen as solvents. Findings: The results showed 50% ethanol (v/v) was the best solvent for all three herbs in terms of extraction yield. For cold maceration, the extract yields were 14.3%, 17.4% and 7.6% for P. niruri, O. stamineus and L. pumila, respectively. Whilst for soxhlet method, the same trend was observed where 50% ethanol (v/v) gave the highest extract yield of 21.2%, 14.3% and 6.8% for P. niruri, O. stamineus and L. pumila, respectively. Total phenolic content and antioxidant capacity were also highest using 50% ethanol and soxhlet method for all herbs. Application/Improvements: This shows that the food-grade solvents at a certain concentration are suitable for the best extraction of selected herbs.

Original languageEnglish
Article number95235
JournalIndian Journal of Science and Technology
Volume9
Issue number21
DOIs
Publication statusPublished - 2016

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Orthosiphon aristatus
Phyllanthus niruri
herbs
ethanol
maceration
extracts
antioxidants
methodology
food grades
cytotoxicity
statistical analysis
economics

Keywords

  • Labisia pumila
  • Maceration
  • Orthosiphon stamineus
  • Phyllanthus niruri
  • Soxhlet

ASJC Scopus subject areas

  • General

Cite this

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title = "Effects of solvents and extraction methods on herbal plants Phyllanthus niruri, Orthosiphon stamineus and Labisia pumila",
abstract = "Background/Objectives: Phyllanthus niruri, Orthosiphon stamineus and Labisia pumila are the three herbs listed in Malaysian National Key Economic Areas (NKEA). This study was conducted to determine the herbs extract yields and activities on antioxidant and cytotoxicity properties. Methods/Statistical Analysis: Cold maceration and soxhlet were the extraction methods employed and water, ethanol and 50{\%} ethanol (v/v) were chosen as solvents. Findings: The results showed 50{\%} ethanol (v/v) was the best solvent for all three herbs in terms of extraction yield. For cold maceration, the extract yields were 14.3{\%}, 17.4{\%} and 7.6{\%} for P. niruri, O. stamineus and L. pumila, respectively. Whilst for soxhlet method, the same trend was observed where 50{\%} ethanol (v/v) gave the highest extract yield of 21.2{\%}, 14.3{\%} and 6.8{\%} for P. niruri, O. stamineus and L. pumila, respectively. Total phenolic content and antioxidant capacity were also highest using 50{\%} ethanol and soxhlet method for all herbs. Application/Improvements: This shows that the food-grade solvents at a certain concentration are suitable for the best extraction of selected herbs.",
keywords = "Labisia pumila, Maceration, Orthosiphon stamineus, Phyllanthus niruri, Soxhlet",
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AU - Latip, Jalifah

PY - 2016

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AB - Background/Objectives: Phyllanthus niruri, Orthosiphon stamineus and Labisia pumila are the three herbs listed in Malaysian National Key Economic Areas (NKEA). This study was conducted to determine the herbs extract yields and activities on antioxidant and cytotoxicity properties. Methods/Statistical Analysis: Cold maceration and soxhlet were the extraction methods employed and water, ethanol and 50% ethanol (v/v) were chosen as solvents. Findings: The results showed 50% ethanol (v/v) was the best solvent for all three herbs in terms of extraction yield. For cold maceration, the extract yields were 14.3%, 17.4% and 7.6% for P. niruri, O. stamineus and L. pumila, respectively. Whilst for soxhlet method, the same trend was observed where 50% ethanol (v/v) gave the highest extract yield of 21.2%, 14.3% and 6.8% for P. niruri, O. stamineus and L. pumila, respectively. Total phenolic content and antioxidant capacity were also highest using 50% ethanol and soxhlet method for all herbs. Application/Improvements: This shows that the food-grade solvents at a certain concentration are suitable for the best extraction of selected herbs.

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