Effects of preeclampsia on the yield of hematopoietic stem cells obtained from umbilical cord blood at delivery

S Fadilah S. Abdul Wahid, Mona Zaria Nasaruddin, Mohd Razif Mohd Idris, Maiza Tusimin, Nor Rafeah Tumian, Zaleha Abdullah Mahdy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aim: To compare the numbers of cord blood CD34+ hematopoietic stem cells (HSC) between preeclampsia (PE) and control (non-PE) subjects and to determine the factors that may influence this observation. Methods: Umbilical cord blood was collected from 28 PE and 19 non-PE subjects. Nucleated and CD34+ cell counts were derived using the Trucount tube-based stem cell enumeration kit on BD FACSCalibur. Results: The cord blood volume, nucleated and CD34+ cell counts were significantly reduced in PE subjects compared to non-PE subjects. Among the PE subjects, systolic and diastolic blood pressure demonstrated a negative correlation with total nucleated and CD34+ cell counts. Gestational age at delivery influenced cord blood volume and nucleated cell counts, but not CD34+ cell counts. Birth weight and placental weight correlated strongly with cord blood volume, and nucleated and CD34+ cell counts. There were no correlations observed between cord blood parameters and maternal age, maternal white cell count, gravidity, route of delivery or neonatal gender among PE subjects. Conclusion: Preeclampsia has a negative impact on the yield of HSC obtained from cord blood at delivery. Maternal blood pressure, neonatal birth weight and placental weight are important factors influencing the numbers of cord blood HSC. These findings should be taken into consideration when selecting cord blood units from mothers with PE for banking. Selecting the heaviest term neonate might improve the yield of cord blood HSC obtained from PE mothers.

Original languageEnglish
Pages (from-to)490-497
Number of pages8
JournalJournal of Obstetrics and Gynaecology Research
Volume38
Issue number3
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Hematopoietic Stem Cells
Pre-Eclampsia
Fetal Blood
Cell Count
Blood Volume
Blood Pressure
Birth Weight
Mothers
Gravidity
Weights and Measures
Maternal Age
Gestational Age
Stem Cells

Keywords

  • CD34+ hematopoietic stem cell
  • Cord blood
  • Cord blood banking
  • Nucleated cell count
  • Preeclampsia

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Effects of preeclampsia on the yield of hematopoietic stem cells obtained from umbilical cord blood at delivery. / S. Abdul Wahid, S Fadilah; Nasaruddin, Mona Zaria; Idris, Mohd Razif Mohd; Tusimin, Maiza; Tumian, Nor Rafeah; Abdullah Mahdy, Zaleha.

In: Journal of Obstetrics and Gynaecology Research, Vol. 38, No. 3, 03.2012, p. 490-497.

Research output: Contribution to journalArticle

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AU - Abdullah Mahdy, Zaleha

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N2 - Aim: To compare the numbers of cord blood CD34+ hematopoietic stem cells (HSC) between preeclampsia (PE) and control (non-PE) subjects and to determine the factors that may influence this observation. Methods: Umbilical cord blood was collected from 28 PE and 19 non-PE subjects. Nucleated and CD34+ cell counts were derived using the Trucount tube-based stem cell enumeration kit on BD FACSCalibur. Results: The cord blood volume, nucleated and CD34+ cell counts were significantly reduced in PE subjects compared to non-PE subjects. Among the PE subjects, systolic and diastolic blood pressure demonstrated a negative correlation with total nucleated and CD34+ cell counts. Gestational age at delivery influenced cord blood volume and nucleated cell counts, but not CD34+ cell counts. Birth weight and placental weight correlated strongly with cord blood volume, and nucleated and CD34+ cell counts. There were no correlations observed between cord blood parameters and maternal age, maternal white cell count, gravidity, route of delivery or neonatal gender among PE subjects. Conclusion: Preeclampsia has a negative impact on the yield of HSC obtained from cord blood at delivery. Maternal blood pressure, neonatal birth weight and placental weight are important factors influencing the numbers of cord blood HSC. These findings should be taken into consideration when selecting cord blood units from mothers with PE for banking. Selecting the heaviest term neonate might improve the yield of cord blood HSC obtained from PE mothers.

AB - Aim: To compare the numbers of cord blood CD34+ hematopoietic stem cells (HSC) between preeclampsia (PE) and control (non-PE) subjects and to determine the factors that may influence this observation. Methods: Umbilical cord blood was collected from 28 PE and 19 non-PE subjects. Nucleated and CD34+ cell counts were derived using the Trucount tube-based stem cell enumeration kit on BD FACSCalibur. Results: The cord blood volume, nucleated and CD34+ cell counts were significantly reduced in PE subjects compared to non-PE subjects. Among the PE subjects, systolic and diastolic blood pressure demonstrated a negative correlation with total nucleated and CD34+ cell counts. Gestational age at delivery influenced cord blood volume and nucleated cell counts, but not CD34+ cell counts. Birth weight and placental weight correlated strongly with cord blood volume, and nucleated and CD34+ cell counts. There were no correlations observed between cord blood parameters and maternal age, maternal white cell count, gravidity, route of delivery or neonatal gender among PE subjects. Conclusion: Preeclampsia has a negative impact on the yield of HSC obtained from cord blood at delivery. Maternal blood pressure, neonatal birth weight and placental weight are important factors influencing the numbers of cord blood HSC. These findings should be taken into consideration when selecting cord blood units from mothers with PE for banking. Selecting the heaviest term neonate might improve the yield of cord blood HSC obtained from PE mothers.

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