Effects of n-acetyl-cysteine supplementation on ex-vivo clonogenicity and oxidative profile of lineage-committed hematopoietic stem/progenitor cells

Chan Chin Yi, Zariyantey Abd Hamid, Taib Izatus Shima, Tan Hui Yee, Muhd Khairul Akmal Wak Harto, Chow Paik Wah

Research output: Contribution to journalArticle

Abstract

Hematopoietic stem and progenitor cells (HSPCs) are exposed to oxidative damage acquired during ex vivo expansion which affects their therapeutic potency. Efforts to overcome this limitation includes the use of antioxidants. The effects of N-Acetyl-Cysteine (NAC) supplementation for 48 hours on maintenance of ex vivo HSPCs was investigated by examining the cell viability at concentrations of 0.125 µM, 0.25 µM, 0.5 µM, 1.0 µM and 2.0 µM, followed by clonogenicity and oxidative status assessments of lineage-committed progenitors (myeloid, erythroid and pre-B lymphoid) at selected NAC concentrations (0.25 µM, 0.5 µM, 2.0 µM). NAC supplementation significantly (p< 0.05) enhanced viability of HSPC at 0.25 µM, 0.5 µM, 2.0 µM. The clonogenicity of each progenitor was not affected as no significant changes of Colony Forming Units (CFUs) counts was noted between NAC-supplemented group than control. NAC showed no significant effects on reactive oxygen species (ROS), glutathione (GSH) and superoxide dismutase (SOD) levels of respective progenitors as compared to control. Conclusively, NAC shows potential property as antioxidant supplement for ex vivo maintenance of HSPCs by promoting survivability and maintaining clonogenicity.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJurnal Teknologi
Volume80
Issue number3
DOIs
Publication statusPublished - 1 May 2018

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Acetylcysteine
Stem cells
Antioxidants
Cells
Oxygen

Keywords

  • Clonogenicity
  • Ex vivo
  • Hematopoietic stem/progenitor cells
  • N-Acetyl-Cysteine
  • Oxidative profile

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Effects of n-acetyl-cysteine supplementation on ex-vivo clonogenicity and oxidative profile of lineage-committed hematopoietic stem/progenitor cells. / Chin Yi, Chan; Abd Hamid, Zariyantey; Izatus Shima, Taib; Yee, Tan Hui; Wak Harto, Muhd Khairul Akmal; Wah, Chow Paik.

In: Jurnal Teknologi, Vol. 80, No. 3, 01.05.2018, p. 1-8.

Research output: Contribution to journalArticle

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