Effects of Mg2+, Fe3+, Mn2+ and Cu 2+ ions on lipid accumulation by cunninghamella bainieri 2A1

Vidyah Manikan, Mohd. Sahaid Kalil, Othman Omar, Abdul Jalil Abdul Kader, Aidil Abdul Hamid

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu 2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistical method (Response Surface Methodology - RSM) to study the combined effects of Mg2+, Fe3+, Mn2+ and Cu2+ on lipid accumulation of C. baineri 2A1. Cultivation was carried out in 250 mL Erlenmeyer flasks containing 100 mL nitrogen limited medium at 30°C and 250 rpm agitation for 120 h. A thirty-run central composite design experiment was employed to identify and optimize the significant factors. In addition to Mg2+ and Fe 3+ which were shown to have significant effects on lipid accumulation, the interactions between Mg2+ and Cu2+, as well as the effect of Cu2+ in quadratic terms were also found to have significant effect on the process (p<0.05). The highest amount of lipid obtained in this study was 39% g/g biomass with optimal levels of Mg 2+, Fe3+ and Cu2+ at 5.00, 0.017 and 0.0005 g/L, respectively, while Mn2+ was omitted. A 32% increment in lipid yield was recorded, where the lipid content increased to 38%, compared to initial yield of 29% g/g biomass prior to optimization. In conclusion, Mg 2+ and Fe3+ have significant positive effect on the lipid accumulation of this fungus, whereas Mn2+ and Cu2+ exert negative effects in combination.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalSains Malaysiana
Volume43
Issue number3
Publication statusPublished - Mar 2014

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Cunninghamella
ions
lipids
oleaginous fungi
fungi
biomass
agitation
response surface methodology
metal ions
statistical analysis
culture media
experimental design
lipid content

Keywords

  • Cunninghamella
  • Metal ions
  • Response surface methodology
  • Single cell oil

ASJC Scopus subject areas

  • General

Cite this

Effects of Mg2+, Fe3+, Mn2+ and Cu 2+ ions on lipid accumulation by cunninghamella bainieri 2A1. / Manikan, Vidyah; Kalil, Mohd. Sahaid; Omar, Othman; Kader, Abdul Jalil Abdul; Abdul Hamid, Aidil.

In: Sains Malaysiana, Vol. 43, No. 3, 03.2014, p. 443-449.

Research output: Contribution to journalArticle

Manikan, Vidyah ; Kalil, Mohd. Sahaid ; Omar, Othman ; Kader, Abdul Jalil Abdul ; Abdul Hamid, Aidil. / Effects of Mg2+, Fe3+, Mn2+ and Cu 2+ ions on lipid accumulation by cunninghamella bainieri 2A1. In: Sains Malaysiana. 2014 ; Vol. 43, No. 3. pp. 443-449.
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