Effects of metal ion concentrations on lipid and gamma linolenic acid production by Cunninghamella sp. 2A1

Farhila Muhid, Wan Nazatul Naziah Wan Nawi, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effects of different concentration of MgSO4.7H2O, FeCl3.6H2O, ZnSO4.7H2O, CuSO 4.5H2O and MnSO4.5H2O on lipid and GLA production by Cunninghamella sp. 2A1 were studied in submerged culture fermentation. Cultivation was performed in 250 ml conical flask (100ml working volume), at 30°C and 250 rpm agitation for 120h. Mg2+, Fe 2+ and Zn2+ have significant effect on lipid accumulation as inclusion of each ions resulted in a 64, 43 and 33% increase in lipid content compared to cultures which was cultivated with the omission of each ions, respectively. All three metal ions also affected GLA production by Cunninghamella sp. 2A1 with Zn having the most profound effect with an increase of up to 74% of GLA yield (g GLA/g lipidless biomass) achieved. As omission of any single metal ions did not affect biomass concentration achieved, we concluded that metal ions components in production media may have affected lipid and GLA production by affecting pathways involved in the biosynthesis of lipid and GLA. This study reports the critical concentration of each metal ion for optimal lipid and GLA production by Cunninghamella sp. 2A1.

Original languageEnglish
Pages (from-to)62-67
Number of pages6
JournalOnLine Journal of Biological Sciences
Volume8
Issue number3
Publication statusPublished - 2008

Fingerprint

Cunninghamella
gamma-Linolenic Acid
gamma-linolenic acid
metal ions
Metal ions
Metals
Ions
Lipids
lipids
Biomass
ions
biomass
agitation
Biosynthesis
Fermentation
fermentation
lipid content
biosynthesis

Keywords

  • Cunninghamella sp
  • GLA and metal ion
  • Lipid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of metal ion concentrations on lipid and gamma linolenic acid production by Cunninghamella sp. 2A1. / Muhid, Farhila; Nawi, Wan Nazatul Naziah Wan; Kader, Abdul Jalil Abdul; Wan Yusoff, Wan Mohtar; Abdul Hamid, Aidil.

In: OnLine Journal of Biological Sciences, Vol. 8, No. 3, 2008, p. 62-67.

Research output: Contribution to journalArticle

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AU - Wan Yusoff, Wan Mohtar

AU - Abdul Hamid, Aidil

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