Effects of metabolic syndrome on bone mineral density, histomorphometry and remodelling markers in male rats

Sok Kuan Wong, Chin Kok Yong, Farihah Suhaimi, Fairus Ahmad, Ima Nirwana Soelaiman

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Abstract

This study aimed to evaluate the effects of metabolic syndrome (MetS) induced by high-carbohydrate high-fat (HCHF) diet on bone mineral density (BMD), histomorphometry and remodelling markers in male rats. Twelve male Wistar rats aged 12 weeks old were randomized into two groups. The normal group was given standard rat chow while the HCHF group was given HCHF diet to induce MetS. Abdominal circumference, blood glucose, blood pressure, and lipid profile were measured for the confirmation of MetS. Bone mineral density, histomorphometry and remodelling markers were evaluated for the confirmation of bone loss. The HCHF diet caused central obesity, hyperglycaemia, hypertension, and dyslipidaemia in male rats. No significant difference was observed in whole body bone mineral content and BMD between the normal and HCHF rats (p>0.05). For bone histomorphometric parameters, HCHF diet-fed animals had significantly lower osteoblast surface, osteoid surface, osteoid volume, and significantly higher eroded surface; resulting in a reduction in trabecular bone volume (p<0.05). Feeding on HCHF diet caused a significantly higher CTX-1 level (p<0.05), but did not cause any significant change in osteocalcin level compared to normal rats (p>0.05). In conclusion, HCHF diet-induced MetS causes imbalance in bone remodelling, leading to the deterioration of trabecular bone structure.

Original languageEnglish
Article numbere0192416
JournalPLoS One
Volume13
Issue number2
DOIs
Publication statusPublished - 1 Feb 2018

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Bone Remodeling
metabolic syndrome
bone density
high carbohydrate diet
Bone Density
Minerals
high fat diet
Rats
Bone
High Fat Diet
Carbohydrates
bones
Fats
Nutrition
rats
carbohydrates
osteoblasts
Bone and Bones
hyperlipidemia
hyperglycemia

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Effects of metabolic syndrome on bone mineral density, histomorphometry and remodelling markers in male rats. / Wong, Sok Kuan; Kok Yong, Chin; Suhaimi, Farihah; Ahmad, Fairus; Soelaiman, Ima Nirwana.

In: PLoS One, Vol. 13, No. 2, e0192416, 01.02.2018.

Research output: Contribution to journalArticle

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