Effects of Lunasia amara blanco (Sanrego) on male fertility

A preliminary study on sperm proteomic analysis

Muhammad Ja far Luthfi, Amira Kamalrudin, Mahanem Mat Noor

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lunasia amara Blanco or sanrego has long been applied as a pro-fertility agent in folk medicine and it has also been scientifically proven to improve sperm quality in rats. Nevertheless, little is known about the underlying molecular mechanism of improved sperm function upon treatment with sanrego. As such, this study looked into the potential proteomic changes in sperm treated with 60 mg/kg body weight sanrego aqueous extract, in comparison to control. The sperm proteins were subjected to proteomic analysis using 2-D SDS PAGE and MALDI-TOF. The comparison of the sperm protein profiles between treated and control rats revealed two downregulated proteins (cenexin-1 and GLUL) and 11 upregulated proteins (Eno1,PGK2,F1-ATP synthase, FO-ATP synthase, LDHC, GLUL1, ODF2, Sp17, asparaginase, DNAJB13, and tubulin) in treated rats. These proteins have been identified to be mainly involved in energy metabolism, sperm motility, amino acid metabolism, and cell signalling. This improved sperm function in sanrego-treated rats may, thus, be a direct result of proteomic changes, as discovered in this present study.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalJournal of Applied Pharmaceutical Science
Volume7
Issue number8
DOIs
Publication statusPublished - 1 Aug 2017

Fingerprint

Proteomics
Fertility
Spermatozoa
Proteins
Adenosine Triphosphate
Fertility Agents
Asparaginase
Sperm Motility
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Traditional Medicine
Tubulin
Energy Metabolism
Polyacrylamide Gel Electrophoresis
Down-Regulation
Body Weight
Amino Acids

Keywords

  • Lunasia amara
  • Male fertility
  • Proteomics
  • Sanrego
  • Sperm proteins

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

Effects of Lunasia amara blanco (Sanrego) on male fertility : A preliminary study on sperm proteomic analysis. / Luthfi, Muhammad Ja far; Kamalrudin, Amira; Mat Noor, Mahanem.

In: Journal of Applied Pharmaceutical Science, Vol. 7, No. 8, 01.08.2017, p. 85-91.

Research output: Contribution to journalArticle

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