Effects of local microbial bioaugmentation and biostimulation on the bioremediation of total petroleum hydrocarbons (TPH) in crude oil contaminated soil based on laboratory and field observations

Fatihah Suja`, Fazli Rahim, Mohd. Raihan Taha, Nuraini Hambali, M. Rizal Razali, Alia Khalid, Ainon Hamzah

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Abstract

This study investigated factors enhancing the performance of the bioremediation of Total Petroleum Hydrocarbons (TPHs) in crude oil-contaminated soil in laboratory and field observations. The bioaugmentation process used local microbial consortia (MC1, MC2 and MC3) combined with the biostimulation processes of nutrient addition (mineral-salt medium, MSM and NPK) and enhanced air stimulation (air supply and Oxygen Releasing Compound (ORC™)). The microcosm tests were conducted in tank and soil column setups, whereas the field test was performed in test plots inside an oil and gas facility in Malaysia. In the microcosm tank experiment, the combination of bioaugmentation (10% inoculum size of MC3) and MSM biostimulation yielded the highest TPH degradation of 79% of the total. In the column experiments, the degradation of TPHs in the top soil was highest in columns combining bioaugmentation and nutrient addition, whereas in the bottom soil, the degradation of TPHs was highest in columns combining bioaugmentation with the addition of both nutrients and ORCs. In the field demonstration, 97% of the TPHs were degraded in the top soil (0-1m) when bioaugmented with MC2. The kinetic analysis study of the microcosm tank showed that a combination of both biostimulation and bioaugmentation in the soil column achieved the fastest rate constant of 0.0390day-1. The field test also demonstrated a comparable rate constant of 0.0339day-1. The kinetic rate constants in both the laboratory and field indicated that the best treatment method for the contaminated site is a combination of MC3 bioaugmentation and nutrient biostimulation.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalInternational Biodeterioration and Biodegradation
Volume90
DOIs
Publication statusPublished - 2014

Fingerprint

Environmental Biodegradation
Bioremediation
Petroleum
petroleum hydrocarbon
Hydrocarbons
bioremediation
crude oil
Soil
Crude oil
Soils
Nutrients
microcosm
nutrient
Food
Rate constants
soil column
degradation
topsoil
Degradation
Oxygen Compounds

Keywords

  • Bioaugmentation
  • Bioremediation
  • Biostimulation
  • Biotechnology
  • Total petroleum hydrocarbon

ASJC Scopus subject areas

  • Waste Management and Disposal
  • Microbiology
  • Biomaterials

Cite this

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title = "Effects of local microbial bioaugmentation and biostimulation on the bioremediation of total petroleum hydrocarbons (TPH) in crude oil contaminated soil based on laboratory and field observations",
abstract = "This study investigated factors enhancing the performance of the bioremediation of Total Petroleum Hydrocarbons (TPHs) in crude oil-contaminated soil in laboratory and field observations. The bioaugmentation process used local microbial consortia (MC1, MC2 and MC3) combined with the biostimulation processes of nutrient addition (mineral-salt medium, MSM and NPK) and enhanced air stimulation (air supply and Oxygen Releasing Compound (ORC™)). The microcosm tests were conducted in tank and soil column setups, whereas the field test was performed in test plots inside an oil and gas facility in Malaysia. In the microcosm tank experiment, the combination of bioaugmentation (10{\%} inoculum size of MC3) and MSM biostimulation yielded the highest TPH degradation of 79{\%} of the total. In the column experiments, the degradation of TPHs in the top soil was highest in columns combining bioaugmentation and nutrient addition, whereas in the bottom soil, the degradation of TPHs was highest in columns combining bioaugmentation with the addition of both nutrients and ORCs. In the field demonstration, 97{\%} of the TPHs were degraded in the top soil (0-1m) when bioaugmented with MC2. The kinetic analysis study of the microcosm tank showed that a combination of both biostimulation and bioaugmentation in the soil column achieved the fastest rate constant of 0.0390day-1. The field test also demonstrated a comparable rate constant of 0.0339day-1. The kinetic rate constants in both the laboratory and field indicated that the best treatment method for the contaminated site is a combination of MC3 bioaugmentation and nutrient biostimulation.",
keywords = "Bioaugmentation, Bioremediation, Biostimulation, Biotechnology, Total petroleum hydrocarbon",
author = "Fatihah Suja` and Fazli Rahim and Taha, {Mohd. Raihan} and Nuraini Hambali and {Rizal Razali}, M. and Alia Khalid and Ainon Hamzah",
year = "2014",
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language = "English",
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pages = "115--122",
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T1 - Effects of local microbial bioaugmentation and biostimulation on the bioremediation of total petroleum hydrocarbons (TPH) in crude oil contaminated soil based on laboratory and field observations

AU - Suja`, Fatihah

AU - Rahim, Fazli

AU - Taha, Mohd. Raihan

AU - Hambali, Nuraini

AU - Rizal Razali, M.

AU - Khalid, Alia

AU - Hamzah, Ainon

PY - 2014

Y1 - 2014

N2 - This study investigated factors enhancing the performance of the bioremediation of Total Petroleum Hydrocarbons (TPHs) in crude oil-contaminated soil in laboratory and field observations. The bioaugmentation process used local microbial consortia (MC1, MC2 and MC3) combined with the biostimulation processes of nutrient addition (mineral-salt medium, MSM and NPK) and enhanced air stimulation (air supply and Oxygen Releasing Compound (ORC™)). The microcosm tests were conducted in tank and soil column setups, whereas the field test was performed in test plots inside an oil and gas facility in Malaysia. In the microcosm tank experiment, the combination of bioaugmentation (10% inoculum size of MC3) and MSM biostimulation yielded the highest TPH degradation of 79% of the total. In the column experiments, the degradation of TPHs in the top soil was highest in columns combining bioaugmentation and nutrient addition, whereas in the bottom soil, the degradation of TPHs was highest in columns combining bioaugmentation with the addition of both nutrients and ORCs. In the field demonstration, 97% of the TPHs were degraded in the top soil (0-1m) when bioaugmented with MC2. The kinetic analysis study of the microcosm tank showed that a combination of both biostimulation and bioaugmentation in the soil column achieved the fastest rate constant of 0.0390day-1. The field test also demonstrated a comparable rate constant of 0.0339day-1. The kinetic rate constants in both the laboratory and field indicated that the best treatment method for the contaminated site is a combination of MC3 bioaugmentation and nutrient biostimulation.

AB - This study investigated factors enhancing the performance of the bioremediation of Total Petroleum Hydrocarbons (TPHs) in crude oil-contaminated soil in laboratory and field observations. The bioaugmentation process used local microbial consortia (MC1, MC2 and MC3) combined with the biostimulation processes of nutrient addition (mineral-salt medium, MSM and NPK) and enhanced air stimulation (air supply and Oxygen Releasing Compound (ORC™)). The microcosm tests were conducted in tank and soil column setups, whereas the field test was performed in test plots inside an oil and gas facility in Malaysia. In the microcosm tank experiment, the combination of bioaugmentation (10% inoculum size of MC3) and MSM biostimulation yielded the highest TPH degradation of 79% of the total. In the column experiments, the degradation of TPHs in the top soil was highest in columns combining bioaugmentation and nutrient addition, whereas in the bottom soil, the degradation of TPHs was highest in columns combining bioaugmentation with the addition of both nutrients and ORCs. In the field demonstration, 97% of the TPHs were degraded in the top soil (0-1m) when bioaugmented with MC2. The kinetic analysis study of the microcosm tank showed that a combination of both biostimulation and bioaugmentation in the soil column achieved the fastest rate constant of 0.0390day-1. The field test also demonstrated a comparable rate constant of 0.0339day-1. The kinetic rate constants in both the laboratory and field indicated that the best treatment method for the contaminated site is a combination of MC3 bioaugmentation and nutrient biostimulation.

KW - Bioaugmentation

KW - Bioremediation

KW - Biostimulation

KW - Biotechnology

KW - Total petroleum hydrocarbon

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U2 - 10.1016/j.ibiod.2014.03.006

DO - 10.1016/j.ibiod.2014.03.006

M3 - Article

VL - 90

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JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

SN - 0964-8305

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