Effects of light and temperature on growth, nitrate uptake, and toxin production of two tropical dinoflagellates: Alexandrium tamiyavanichii and Alexandrium minutum (Dinophyceae)

Po Teen Lim, Chui Pin Leaw, Gires Usup, Atsushi Kobiyama, Kazuhiko Koike, Takehiko Ogata

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The two tropical estuarine dinoflagellates, Alexandrium tamiyavanichii Balech and A. minutum Halim, were used to determine the ecophysiological adaptations in relation to their temperate counterparts. These species are the two main causative organisms responsible for the incidence of paralytic shellfish poisoning (PSP) in Southeast Asia. The effects of light (10, 40, 60, and 100 μmol photons·m-2·s-1) and temperature (15, 20, and 25°C) on the growth, nitrate assimilation, and PST production of these species were investigated in clonal batch cultures over the growth cycle. The growth rates of A. tamiyavanichii and A. minutum increased with increasing temperature and irradiance. The growth of A. tamiyavanichii was depressed at lower temperature (20°C) and irradiance (40 μmol photons·m-2·s-1). Both species showed no net growth at 10 μmol photons·m-2·s-1 and a temperature of 15°C, although cells remained alive. Cellular toxin quotas (Qt) of A. tamiyavanichii and A. minutum varied in the range of 60-180 and 10-42 fmol PST·cell-1, respectively. Toxin production rate, Rtox, increased with elevated light at both 20 and 25°C, with a pronounced effect observed at exponential phase in both species (A. tamiyavanichii, r2 = 0.95; A. minutum, r2 = 0.96). Toxin production rate also increased significantly with elevated temperature (P < 0.05) for both species examined. We suggest that the ecotypic variations in growth adaptations and toxin production of these Malaysian strains may reveal a unique physiological adaptation of tropical Alexandrium species.

Original languageEnglish
Pages (from-to)786-799
Number of pages14
JournalJournal of Phycology
Volume42
Issue number4
DOIs
Publication statusPublished - Aug 2006

Fingerprint

Alexandrium
dinoflagellate
toxin
Dinophyceae
toxins
nitrates
nitrate
temperature
irradiance
paralytic shellfish poisoning
angle of incidence
South East Asia
Alexandrium minutum
Miozoa
effect
organisms
cells

Keywords

  • Alexandrium minutum
  • Alexandrium tamiyavanichii
  • Growth
  • Light
  • Temperature
  • Toxin production
  • Tropical

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Effects of light and temperature on growth, nitrate uptake, and toxin production of two tropical dinoflagellates : Alexandrium tamiyavanichii and Alexandrium minutum (Dinophyceae). / Lim, Po Teen; Leaw, Chui Pin; Usup, Gires; Kobiyama, Atsushi; Koike, Kazuhiko; Ogata, Takehiko.

In: Journal of Phycology, Vol. 42, No. 4, 08.2006, p. 786-799.

Research output: Contribution to journalArticle

Lim, Po Teen ; Leaw, Chui Pin ; Usup, Gires ; Kobiyama, Atsushi ; Koike, Kazuhiko ; Ogata, Takehiko. / Effects of light and temperature on growth, nitrate uptake, and toxin production of two tropical dinoflagellates : Alexandrium tamiyavanichii and Alexandrium minutum (Dinophyceae). In: Journal of Phycology. 2006 ; Vol. 42, No. 4. pp. 786-799.
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