Effects of leptin on sperm count and morphology in Sprague-Dawley rats and their reversibility following a 6-week recovery period

F. A. Almabhouh, K. Osman, I. Siti Fatimah, G. Sergey, J. Gnanou, H. J. Singh

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Altered epididymal sperm count and morphology following leptin treatment has been reported recently. This study examined the effects of 42 days of leptin treatment on sperm count and morphology and their reversibility during a subsequent 56-day recovery period. Twelve-week-old male Sprague-Dawley rats were randomised into four leptin and four saline-treated control groups (n = 6). Intraperitoneal injections of leptin were given daily (60 μg Kg-1 body weight) for 42 days. Controls received 0.1 ml of 0.9% saline. Leptin-treated animals and their respective age-matched controls were euthanised on either day 1, 21, 42 or 56 of recovery for collection of epididymal spermatozoa. Sperm concentration was determined using a Makler counting chamber. Spermatozoa were analysed for 8-hydroxy-2-deoxyguanosine and DNA fragmentation (Comet assay). Data were analysed using anova. Sperm concentration was significantly lower but fraction of abnormal spermatozoa, and levels of 8-hydroxy-2-deoxyguanosine were significantly higher in leptin-treated rats on day 1 of recovery. Comet assays revealed significant DNA fragmentation in leptin-treated rats. These differences were reduced by day 56 of recovery. It appears that 42 days of leptin treatment to Sprague-Dawley rats has significant adverse effects on sperm count and morphology that reverse following discontinuation of leptin treatment.

Original languageEnglish
JournalAndrologia
DOIs
Publication statusAccepted/In press - 2014

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Sperm Count
Leptin
Sprague Dawley Rats
Spermatozoa
Comet Assay
DNA Fragmentation
Therapeutics
Intraperitoneal Injections
Body Weight
Control Groups

Keywords

  • 8-OHdG
  • DNA fragmentation
  • Leptin
  • Sperm count
  • Sperm morphology

ASJC Scopus subject areas

  • Urology
  • Endocrinology

Cite this

Effects of leptin on sperm count and morphology in Sprague-Dawley rats and their reversibility following a 6-week recovery period. / Almabhouh, F. A.; Osman, K.; Siti Fatimah, I.; Sergey, G.; Gnanou, J.; Singh, H. J.

In: Andrologia, 2014.

Research output: Contribution to journalArticle

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AU - Sergey, G.

AU - Gnanou, J.

AU - Singh, H. J.

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