Effects of intravenous palm oil-based lipid nanoemulsion on fat metabolism in rabbits

Mahdi Jufri, Norazrina Azmi, Ahmad Fuad Shamsuddin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: To investigate the effects of a newly-developed nanoemulsion of palm oil in combination with medium chain triglyceride (MCT) oil (NEMS®MCT/LCT20%) on fat metabolism in male New Zealand white rabbits. Methods: Six rabbits were divided into two groups of three rabbits in each group. NEMS®MCT/LCT20%, Lipofundin®MCT/LCT 20% (a commercially-available lipid emulsion) and normal saline (control) were administered intravenously in these rabbits via the marginal ear vein for 6 h. Cross-over method was used in these rabbits with a wash-out period of two weeks in-between infusions. Triglyceride (TG), total cholesterol, low density lipoprotein and high density lipoprotein cholesterols were determined using enzymatic strip test at 0, 2, 4 and 6 h of infusion. Blood levels of free fatty acid (FFA) were measured at 0 and 6 h. Results: Serum TG levels increased in these rabbits after 2 and 4 h infusion of NEMS®MCT/LCT20% but returned to normal after 6 h. Concentration of FFA increased in a dose-related manner but remained within the normal range (315-535 μmol/L). Similar results were obtained with Lipofundin®MCT/LCT20%. All parameters of fat metabolism in these rabbits remained unchanged from baseline when normal saline was administered. Changes in the parameters of fat metabolism measured between NEMS®MCT/LCT20% and Lipofundin®MCT/LCT20% did not show any significant difference. Significant difference (P<0.05) was observed in these parameters when NEMS®MCT/LCT20% was compared to normal saline. Conclusions: Intravenous administration of NEMS®MCT/LCT20% did not cause a permanent increase in the TG level of rabbits while FFA remained within the normal range.

Original languageEnglish
JournalAsian Pacific Journal of Tropical Disease
Volume2
Issue numberSUPPL2
DOIs
Publication statusPublished - 2012

Fingerprint

Fats
Rabbits
Lipids
Triglycerides
Nonesterified Fatty Acids
Reference Values
palm oil
Emulsions
Intravenous Administration
LDL Cholesterol
HDL Cholesterol
Ear
Veins
Oils
Serum

Keywords

  • Fat metabolism
  • Free fatty acid
  • Intravenous
  • Lipofundin®MCT/LCT20%
  • Medium chain triglyceride
  • Nanoemulsion
  • NEMS®MCT/LCT20%
  • Palm oil
  • Serum triglyceride

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Effects of intravenous palm oil-based lipid nanoemulsion on fat metabolism in rabbits. / Jufri, Mahdi; Azmi, Norazrina; Shamsuddin, Ahmad Fuad.

In: Asian Pacific Journal of Tropical Disease, Vol. 2, No. SUPPL2, 2012.

Research output: Contribution to journalArticle

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abstract = "Objective: To investigate the effects of a newly-developed nanoemulsion of palm oil in combination with medium chain triglyceride (MCT) oil (NEMS{\circledR}MCT/LCT20{\%}) on fat metabolism in male New Zealand white rabbits. Methods: Six rabbits were divided into two groups of three rabbits in each group. NEMS{\circledR}MCT/LCT20{\%}, Lipofundin{\circledR}MCT/LCT 20{\%} (a commercially-available lipid emulsion) and normal saline (control) were administered intravenously in these rabbits via the marginal ear vein for 6 h. Cross-over method was used in these rabbits with a wash-out period of two weeks in-between infusions. Triglyceride (TG), total cholesterol, low density lipoprotein and high density lipoprotein cholesterols were determined using enzymatic strip test at 0, 2, 4 and 6 h of infusion. Blood levels of free fatty acid (FFA) were measured at 0 and 6 h. Results: Serum TG levels increased in these rabbits after 2 and 4 h infusion of NEMS{\circledR}MCT/LCT20{\%} but returned to normal after 6 h. Concentration of FFA increased in a dose-related manner but remained within the normal range (315-535 μmol/L). Similar results were obtained with Lipofundin{\circledR}MCT/LCT20{\%}. All parameters of fat metabolism in these rabbits remained unchanged from baseline when normal saline was administered. Changes in the parameters of fat metabolism measured between NEMS{\circledR}MCT/LCT20{\%} and Lipofundin{\circledR}MCT/LCT20{\%} did not show any significant difference. Significant difference (P<0.05) was observed in these parameters when NEMS{\circledR}MCT/LCT20{\%} was compared to normal saline. Conclusions: Intravenous administration of NEMS{\circledR}MCT/LCT20{\%} did not cause a permanent increase in the TG level of rabbits while FFA remained within the normal range.",
keywords = "Fat metabolism, Free fatty acid, Intravenous, Lipofundin{\circledR}MCT/LCT20{\%}, Medium chain triglyceride, Nanoemulsion, NEMS{\circledR}MCT/LCT20{\%}, Palm oil, Serum triglyceride",
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AU - Azmi, Norazrina

AU - Shamsuddin, Ahmad Fuad

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N2 - Objective: To investigate the effects of a newly-developed nanoemulsion of palm oil in combination with medium chain triglyceride (MCT) oil (NEMS®MCT/LCT20%) on fat metabolism in male New Zealand white rabbits. Methods: Six rabbits were divided into two groups of three rabbits in each group. NEMS®MCT/LCT20%, Lipofundin®MCT/LCT 20% (a commercially-available lipid emulsion) and normal saline (control) were administered intravenously in these rabbits via the marginal ear vein for 6 h. Cross-over method was used in these rabbits with a wash-out period of two weeks in-between infusions. Triglyceride (TG), total cholesterol, low density lipoprotein and high density lipoprotein cholesterols were determined using enzymatic strip test at 0, 2, 4 and 6 h of infusion. Blood levels of free fatty acid (FFA) were measured at 0 and 6 h. Results: Serum TG levels increased in these rabbits after 2 and 4 h infusion of NEMS®MCT/LCT20% but returned to normal after 6 h. Concentration of FFA increased in a dose-related manner but remained within the normal range (315-535 μmol/L). Similar results were obtained with Lipofundin®MCT/LCT20%. All parameters of fat metabolism in these rabbits remained unchanged from baseline when normal saline was administered. Changes in the parameters of fat metabolism measured between NEMS®MCT/LCT20% and Lipofundin®MCT/LCT20% did not show any significant difference. Significant difference (P<0.05) was observed in these parameters when NEMS®MCT/LCT20% was compared to normal saline. Conclusions: Intravenous administration of NEMS®MCT/LCT20% did not cause a permanent increase in the TG level of rabbits while FFA remained within the normal range.

AB - Objective: To investigate the effects of a newly-developed nanoemulsion of palm oil in combination with medium chain triglyceride (MCT) oil (NEMS®MCT/LCT20%) on fat metabolism in male New Zealand white rabbits. Methods: Six rabbits were divided into two groups of three rabbits in each group. NEMS®MCT/LCT20%, Lipofundin®MCT/LCT 20% (a commercially-available lipid emulsion) and normal saline (control) were administered intravenously in these rabbits via the marginal ear vein for 6 h. Cross-over method was used in these rabbits with a wash-out period of two weeks in-between infusions. Triglyceride (TG), total cholesterol, low density lipoprotein and high density lipoprotein cholesterols were determined using enzymatic strip test at 0, 2, 4 and 6 h of infusion. Blood levels of free fatty acid (FFA) were measured at 0 and 6 h. Results: Serum TG levels increased in these rabbits after 2 and 4 h infusion of NEMS®MCT/LCT20% but returned to normal after 6 h. Concentration of FFA increased in a dose-related manner but remained within the normal range (315-535 μmol/L). Similar results were obtained with Lipofundin®MCT/LCT20%. All parameters of fat metabolism in these rabbits remained unchanged from baseline when normal saline was administered. Changes in the parameters of fat metabolism measured between NEMS®MCT/LCT20% and Lipofundin®MCT/LCT20% did not show any significant difference. Significant difference (P<0.05) was observed in these parameters when NEMS®MCT/LCT20% was compared to normal saline. Conclusions: Intravenous administration of NEMS®MCT/LCT20% did not cause a permanent increase in the TG level of rabbits while FFA remained within the normal range.

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KW - Palm oil

KW - Serum triglyceride

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