Effects of gold nanoparticles on the response of phenol biosensor containing photocurable membrane with tyrosinase

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The role of incorporation of gold nanoparticles (50-130 nm in diameter) into a series of photocurable methacrylic-acrylic based biosensor membranes containing tyrosinase on the response for phenol detection was investigated. Membranes with different hydrophilicities were prepared from 2-hydroxyethyl methacrylate and n-butyl acrylate via direct photocuring. A range of gold nanoparticles concentrations from 0.01 to 0.5% (w/w) was incorporated into these membranes during the photocuring process. The addition of gold nanoparticles to the biosensor membrane led to improvement in the response time by a reduction of approximately 5 folds to give response times of 5-10 s. The linear response range of the phenol biosensor was also extended from 24 to 90 μM of phenol. The hydrophilicities of the membrane matrices demonstrated strong influence on the biosensor response and appeared to control the effect of the gold nanoparticles. For less hydrophilic methacrylic-acrylic membranes, the addition of gold nanoparticles led to a poorer sensitivity and detection limit of the biosensor towards phenol. Therefore, for the application of gold nanoparticles in the enhancement of a phenol biosensor response, the nanoparticles should be immobilized in a hydrophilic matrix rather than a hydrophobic material.

Original languageEnglish
Pages (from-to)6407-6416
Number of pages10
JournalSensors
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Monophenol Monooxygenase
Biosensing Techniques
Phenol
bioinstrumentation
Biosensors
Gold
phenols
Nanoparticles
Phenols
gold
membranes
Membranes
nanoparticles
Hydrophilicity
Hydrophobic and Hydrophilic Interactions
Reaction Time
Acrylics
Aurothioglucose
matrices
acrylates

Keywords

  • Acrylate
  • Gold nanoparticle
  • Hydrophilic polymer
  • Methacrylate
  • Phenol biosensor
  • Tyrosinase

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Analytical Chemistry
  • Biochemistry

Cite this

Effects of gold nanoparticles on the response of phenol biosensor containing photocurable membrane with tyrosinase. / Abu Hanifah, Sharina; Lee, Yook Heng; Ahmad, Musa.

In: Sensors, Vol. 8, No. 10, 10.2008, p. 6407-6416.

Research output: Contribution to journalArticle

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