Effects of flavonoids on glutathione-S-transferase in human blood platelets, rat liver, rat kidney and HT-29 colon adenocarcinoma cell-lines

Potential in drug metabolism and chemoprevention

Ahmad Rohi Ghazali, R. H. Waring

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Flavonoids are frequently found in fruits and vegetables and are also under investigation as potential chemopreventive agents. We have investigated quercetin, daidzein and genistein for their inhibitory or activating effects on glutathione-S-transferases (GST) from a number of sources. All three compounds inhibited GST activity in human blood platelets, rat liver and rat kidney but induced activity in the HT-29 cell-line derived from the gastrointestinal tract. We also investigated expression of the different subunits of GST in human blood platelets, rat liver, rat kidney and HT-29 cell-lines. The results suggest that while flavonoids can inhibit detoxification pathways in blood platelets, liver and kidney, they increase removal of toxins in the gastrointestinal tract, as they specifically activate the μ- isoform while inhibiting α- and π-GST.

Original languageEnglish
Pages (from-to)449-451
Number of pages3
JournalMedical Science Research
Volume27
Issue number7
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Chemoprevention
Platelets
Glutathione Transferase
Flavonoids
Metabolism
Liver
Rats
Colon
Adenocarcinoma
Blood
Blood Platelets
Kidney
Cell Line
HT29 Cells
Pharmaceutical Preparations
Gastrointestinal Tract
Detoxification
Genistein
Quercetin
Vegetables

Keywords

  • α-
  • μ- and θ-GST isoforms daidzein
  • π-
  • Flavonoids
  • Genistein
  • Glutathione-S-transferase
  • Quercetin

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Effects of flavonoids on glutathione-S-transferase in human blood platelets, rat liver, rat kidney and HT-29 colon adenocarcinoma cell-lines: Potential in drug metabolism and chemoprevention",
abstract = "Flavonoids are frequently found in fruits and vegetables and are also under investigation as potential chemopreventive agents. We have investigated quercetin, daidzein and genistein for their inhibitory or activating effects on glutathione-S-transferases (GST) from a number of sources. All three compounds inhibited GST activity in human blood platelets, rat liver and rat kidney but induced activity in the HT-29 cell-line derived from the gastrointestinal tract. We also investigated expression of the different subunits of GST in human blood platelets, rat liver, rat kidney and HT-29 cell-lines. The results suggest that while flavonoids can inhibit detoxification pathways in blood platelets, liver and kidney, they increase removal of toxins in the gastrointestinal tract, as they specifically activate the μ- isoform while inhibiting α- and π-GST.",
keywords = "α-, μ- and θ-GST isoforms daidzein, π-, Flavonoids, Genistein, Glutathione-S-transferase, Quercetin",
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T2 - Potential in drug metabolism and chemoprevention

AU - Ghazali, Ahmad Rohi

AU - Waring, R. H.

PY - 1999

Y1 - 1999

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AB - Flavonoids are frequently found in fruits and vegetables and are also under investigation as potential chemopreventive agents. We have investigated quercetin, daidzein and genistein for their inhibitory or activating effects on glutathione-S-transferases (GST) from a number of sources. All three compounds inhibited GST activity in human blood platelets, rat liver and rat kidney but induced activity in the HT-29 cell-line derived from the gastrointestinal tract. We also investigated expression of the different subunits of GST in human blood platelets, rat liver, rat kidney and HT-29 cell-lines. The results suggest that while flavonoids can inhibit detoxification pathways in blood platelets, liver and kidney, they increase removal of toxins in the gastrointestinal tract, as they specifically activate the μ- isoform while inhibiting α- and π-GST.

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