Effects of extraction techniques on phenolic components and antioxidant activity of Mengkudu (Morinda citrifolia L.) leaf extracts

Mohd Sabri Pak-Dek, Azizah Osman, Najla Gooda Sahib, Nazamid Saari, Masturah Markom, Azizah Abdul Hamid, Farooq Anwar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The effect of four extraction techniques namely; solvent extraction (SE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and supercritical fluid extraction (SFE) on the phenolic components and antioxidant activity of Morinda citrifolia leaf extracts was investigated. Total phenolics compounds (TPC) were quantified spectrophotometrically using Follin-Ciocalteu regent method, while the catechins were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). Antioxidant activity of the extracts was evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH) capacity and ferric ion reducing antioxidant potential (FRAP). Overall, MAE produced extract exhibited the maximum amount of TPC and catechin while UAE-extract had the highest antioxidant activity. It could be concluded that, even though MAE extract contained high TPC and catechin, the accelerated temperature used during this extraction technique might have attributed to reduce the antioxidant activity of this extract. Therefore, UAE can be recommended for recovery of potent natural antioxidant components from M. citrifolia leaf offering better antioxidant activity.

Original languageEnglish
Pages (from-to)5050-5057
Number of pages8
JournalJournal of Medicinal Plant Research
Volume5
Issue number20
Publication statusPublished - 30 Sep 2011

Fingerprint

Morinda
Morinda citrifolia
leaf extracts
Antioxidants
antioxidant activity
Catechin
Microwaves
Ultrasonics
extracts
phenolic compounds
ultrasonics
methodology
catechin
Supercritical Fluid Chromatography
Reverse-Phase Chromatography
antioxidants
supercritical fluid extraction
reversed-phase liquid chromatography
flavanols
High Pressure Liquid Chromatography

Keywords

  • 2,2-diphenyl-1-picrylhydrazyl (DPPH)
  • Antioxidant extraction
  • Catechin
  • Ferric reducing potential
  • High performance liquid chromatography (HPLC)
  • Morinda citrifolia leaf
  • Total phenolics

ASJC Scopus subject areas

  • Pharmacology
  • Complementary and alternative medicine
  • Drug Discovery
  • Plant Science
  • Pharmaceutical Science

Cite this

Effects of extraction techniques on phenolic components and antioxidant activity of Mengkudu (Morinda citrifolia L.) leaf extracts. / Pak-Dek, Mohd Sabri; Osman, Azizah; Sahib, Najla Gooda; Saari, Nazamid; Markom, Masturah; Hamid, Azizah Abdul; Anwar, Farooq.

In: Journal of Medicinal Plant Research, Vol. 5, No. 20, 30.09.2011, p. 5050-5057.

Research output: Contribution to journalArticle

Pak-Dek, Mohd Sabri ; Osman, Azizah ; Sahib, Najla Gooda ; Saari, Nazamid ; Markom, Masturah ; Hamid, Azizah Abdul ; Anwar, Farooq. / Effects of extraction techniques on phenolic components and antioxidant activity of Mengkudu (Morinda citrifolia L.) leaf extracts. In: Journal of Medicinal Plant Research. 2011 ; Vol. 5, No. 20. pp. 5050-5057.
@article{516eb0621f9a414caa31a11914b74657,
title = "Effects of extraction techniques on phenolic components and antioxidant activity of Mengkudu (Morinda citrifolia L.) leaf extracts",
abstract = "The effect of four extraction techniques namely; solvent extraction (SE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and supercritical fluid extraction (SFE) on the phenolic components and antioxidant activity of Morinda citrifolia leaf extracts was investigated. Total phenolics compounds (TPC) were quantified spectrophotometrically using Follin-Ciocalteu regent method, while the catechins were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). Antioxidant activity of the extracts was evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH) capacity and ferric ion reducing antioxidant potential (FRAP). Overall, MAE produced extract exhibited the maximum amount of TPC and catechin while UAE-extract had the highest antioxidant activity. It could be concluded that, even though MAE extract contained high TPC and catechin, the accelerated temperature used during this extraction technique might have attributed to reduce the antioxidant activity of this extract. Therefore, UAE can be recommended for recovery of potent natural antioxidant components from M. citrifolia leaf offering better antioxidant activity.",
keywords = "2,2-diphenyl-1-picrylhydrazyl (DPPH), Antioxidant extraction, Catechin, Ferric reducing potential, High performance liquid chromatography (HPLC), Morinda citrifolia leaf, Total phenolics",
author = "Pak-Dek, {Mohd Sabri} and Azizah Osman and Sahib, {Najla Gooda} and Nazamid Saari and Masturah Markom and Hamid, {Azizah Abdul} and Farooq Anwar",
year = "2011",
month = "9",
day = "30",
language = "English",
volume = "5",
pages = "5050--5057",
journal = "Journal of Medicinal Plant Research",
issn = "1996-0875",
publisher = "Academic Journals",
number = "20",

}

TY - JOUR

T1 - Effects of extraction techniques on phenolic components and antioxidant activity of Mengkudu (Morinda citrifolia L.) leaf extracts

AU - Pak-Dek, Mohd Sabri

AU - Osman, Azizah

AU - Sahib, Najla Gooda

AU - Saari, Nazamid

AU - Markom, Masturah

AU - Hamid, Azizah Abdul

AU - Anwar, Farooq

PY - 2011/9/30

Y1 - 2011/9/30

N2 - The effect of four extraction techniques namely; solvent extraction (SE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and supercritical fluid extraction (SFE) on the phenolic components and antioxidant activity of Morinda citrifolia leaf extracts was investigated. Total phenolics compounds (TPC) were quantified spectrophotometrically using Follin-Ciocalteu regent method, while the catechins were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). Antioxidant activity of the extracts was evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH) capacity and ferric ion reducing antioxidant potential (FRAP). Overall, MAE produced extract exhibited the maximum amount of TPC and catechin while UAE-extract had the highest antioxidant activity. It could be concluded that, even though MAE extract contained high TPC and catechin, the accelerated temperature used during this extraction technique might have attributed to reduce the antioxidant activity of this extract. Therefore, UAE can be recommended for recovery of potent natural antioxidant components from M. citrifolia leaf offering better antioxidant activity.

AB - The effect of four extraction techniques namely; solvent extraction (SE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and supercritical fluid extraction (SFE) on the phenolic components and antioxidant activity of Morinda citrifolia leaf extracts was investigated. Total phenolics compounds (TPC) were quantified spectrophotometrically using Follin-Ciocalteu regent method, while the catechins were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). Antioxidant activity of the extracts was evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH) capacity and ferric ion reducing antioxidant potential (FRAP). Overall, MAE produced extract exhibited the maximum amount of TPC and catechin while UAE-extract had the highest antioxidant activity. It could be concluded that, even though MAE extract contained high TPC and catechin, the accelerated temperature used during this extraction technique might have attributed to reduce the antioxidant activity of this extract. Therefore, UAE can be recommended for recovery of potent natural antioxidant components from M. citrifolia leaf offering better antioxidant activity.

KW - 2,2-diphenyl-1-picrylhydrazyl (DPPH)

KW - Antioxidant extraction

KW - Catechin

KW - Ferric reducing potential

KW - High performance liquid chromatography (HPLC)

KW - Morinda citrifolia leaf

KW - Total phenolics

UR - http://www.scopus.com/inward/record.url?scp=80054744865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054744865&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:80054744865

VL - 5

SP - 5050

EP - 5057

JO - Journal of Medicinal Plant Research

JF - Journal of Medicinal Plant Research

SN - 1996-0875

IS - 20

ER -