Effects of edible bird's nest (EBN) on cultured rabbit corneal keratocytes

Fadhilah Zainal Abidin, Chua Kien Hui, Ng S. Luan, Elvy Suhana Mohd Ramli, Lee T. Hun, Norzana Abd. Ghafar

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: There has been no effective treatment or agent that is available for corneal injury in promoting corneal wound healing. Previous studies on edible bird's nest extract (EBN) had reported the presence of hormone-like substance; avian epidermal growth factor that could stimulate cell division and enhance regeneration. This study aimed to investigate the effects of EBN on corneal keratocytes proliferative capacity and phenotypical changes.Methods: Corneal keratocytes from six New Zealand White Rabbits were isolated and cultured until Passage 1. The proliferative effects of EBN on corneal keratocytes were determined by MTT assay in serum-containing medium (FDS) and serum-free medium (FD). Keratocytes phenotypical changes were morphologically assessed and gene expression of aldehyde dehydrogenase (ALDH), collagen type 1 and lumican were determined through RT-PCR.Results: The highest cell proliferation was observed when both media were supplemented with 0.05% and 0.1% EBN. Cell proliferation was also consistently higher in FDS compared to FD. Both phase contrast micrographs and gene expression analysis confirmed the corneal keratocytes retained their phenotypes with the addition of EBN.Conclusions: These results suggested that low concentration of EBN could synergistically induce cell proliferation, especially in serum-containing medium. This could be a novel breakthrough as both cell proliferation and functional maintenance are important during corneal wound healing. The in vitro test is considered as a crucial first step for nutri-pharmaceutical formation of EBN-based eye drops before in vivo application.

Original languageEnglish
Article number94
JournalBMC Complementary and Alternative Medicine
Volume11
DOIs
Publication statusPublished - 12 Oct 2011

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Corneal Keratocytes
Birds
Rabbits
Cell Proliferation
Wound Healing
Gene Expression
Aldehyde Dehydrogenase
Ophthalmic Solutions
Serum-Free Culture Media
Collagen Type I
Serum
Epidermal Growth Factor
Cell Division
Regeneration
Maintenance
Hormones
Phenotype
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Complementary and alternative medicine

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Effects of edible bird's nest (EBN) on cultured rabbit corneal keratocytes. / Zainal Abidin, Fadhilah; Kien Hui, Chua; Luan, Ng S.; Mohd Ramli, Elvy Suhana; Hun, Lee T.; Abd. Ghafar, Norzana.

In: BMC Complementary and Alternative Medicine, Vol. 11, 94, 12.10.2011.

Research output: Contribution to journalArticle

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