Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment.

H. Alfaqeh, Chua Kien Hui, B. S. Aminuddin, Ruszymah Idrus

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study aimed to compare the effects of three different media on the in vivo chondrogenesis of sheep bone marrow stem cells (BMSC). Sheep BMSC were cultured in F12:DMEM + 10% FBS, chondrogenic medium containing 5ng/ml TGF,3 + 50ng/ml IGF-1 and UKM-MECC for three weeks. The cultured cells were then harvested for construct formation with fibrin. Constructed tissues were implanted subcutaneously into nude mice for in vivo development. Cell aggregates were formed in both chondrogenic medium and UKM-MECC demonstrated the early chondrogenesis process. After five weeks of in vivo development, both chondrogenic medium and UKM-MECC promoted cartilage matrix synthesis confirmed by Safranin O staining.

Original languageEnglish
Pages (from-to)119-120
Number of pages2
JournalThe Medical journal of Malaysia
Volume63 Suppl A
Publication statusPublished - Jul 2008

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Micellar Electrokinetic Capillary Chromatography
Chondrogenesis
Bone Marrow Cells
Sheep
Stem Cells
Fibrin
Insulin-Like Growth Factor I
Nude Mice
Cartilage
Cultured Cells
Staining and Labeling

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells : histological assessment. / Alfaqeh, H.; Kien Hui, Chua; Aminuddin, B. S.; Idrus, Ruszymah.

In: The Medical journal of Malaysia, Vol. 63 Suppl A, 07.2008, p. 119-120.

Research output: Contribution to journalArticle

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