Effects of culture with TNF-α, TGF-β and insulin on sulphotransferase (SULT 1A1 and 1A3) activity in human colon and neuronal cell lines

E. Davies, C. W. Tsang, Ahmad Rohi Ghazali, R. M. Harris, R. H. Waring

Research output: Contribution to journalArticle

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Abstract

The aim of the study was to determine whether the expression of sulphotransferase enzymes could be affected by the presence of cytokines or peptide hormones. The effects of cytokines (TNF-α and TGF-β) and insulin on sulphotransferase (SULT 1A1 and 1A3) activity were studied in a human neuronal cell line (SK-N-SH) and a human gastrointestinal tract cell line (HT-29). Cells were cultured with varying concentrations of TNF-α, TGF-β or insulin for 24 h; the SULT 1A1 isoform in the 2 cell lines showed different optimal substrate concentrations. There were no direct effects of cytokines on enzyme activity. Culture with TNF-α increased activity of both SULT 1A1 and 1A3 in the HT-29 cells; TGF-β also increased activities of both isoforms but to a lesser extent; insulin increased activity of SULT 1A1 only. The cytokines and insulin had relatively little effect on sulphotransferase activity in the neuronal cell line. These results suggest that, unlike neuronal cells, gastrointestinal cells may respond to physiological states by altering sulphotransferase activity. As certain substrates such as diet-derived heterocyclic amines are bioactivated by sulphation to produce carcinogenic metabolites this may be a factor in the increased incidence of colorectal cancer in patients with inflammatory bowel disease or diabetes.

Original languageEnglish
Pages (from-to)749-754
Number of pages6
JournalToxicology in Vitro
Volume18
Issue number6
DOIs
Publication statusPublished - Dec 2004

Fingerprint

Sulfotransferases
Human Activities
Colon
Cells
Insulin
Cytokines
Cell Line
Protein Isoforms
HT29 Cells
Peptide Hormones
Enzyme activity
Substrates
Enzymes
Nutrition
Medical problems
Metabolites
Inflammatory Bowel Diseases
Amines
Gastrointestinal Tract
Colorectal Neoplasms

Keywords

  • Cytokines
  • Human neuronal and colon cell lines
  • Sulphotransferases

ASJC Scopus subject areas

  • Toxicology

Cite this

Effects of culture with TNF-α, TGF-β and insulin on sulphotransferase (SULT 1A1 and 1A3) activity in human colon and neuronal cell lines. / Davies, E.; Tsang, C. W.; Ghazali, Ahmad Rohi; Harris, R. M.; Waring, R. H.

In: Toxicology in Vitro, Vol. 18, No. 6, 12.2004, p. 749-754.

Research output: Contribution to journalArticle

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