Effects of citrus leaf extract on aortic vascular reactivity in hypertensive rats fed repeatedly heated vegetable oil

Hawa Nordin Siti, Kamisah Yusof, Suhaila Mohamed, Kamsiah Jaarin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The prolonged intake of diet containing repeatedly heated vegetable oil can cause hypertension in the long run. In this study, the effects of citrus leaf extract (CLE) supplementation on vascular reactivity, plasma nitrite, and aortic structure in hypertensive rats that consumed were investigated by the consumption of repeatedly heated vegetable oil. Male Sprague Dawley rats (n = 56) were divided into 7 groups corresponding to the respective diets. For 16 weeks, 1 group was given standard rat chow (control) while other groups were given diets containing 15% w/w of palm oil, fresh palm oil (FPO), palm oil heated 5 times (5HPO), and palm oil heated 10 times (10HPO), with or without the incorporation of 0.15% w/w CLE (FPO+CLE, 5HPO+CLE, or 10HPO+CLE). Plasma nitrite levels were measured before and at 16 weeks of treatment. After 16 weeks, the rats were sacrificed and aortae were harvested for measuring vascular reactivity and for microscopic study. CLE supplementation had significantly reduced the loss of plasma nitrite and attenuated the vasoconstriction response to phenylephrine in the 5HPO group but not in the 10HPO group. However, CLE had no significant effect on the vasorelaxation response to acetylcholine and sodium nitroprusside. The elastic lamellae of tunica media in 5HPO, 10HPO, and 10HPO+CLE groups appeared disorganised and disrupted. Obtained findings suggested that CLE was able to enhance nitric oxide bioavailability that might dampen the vasoconstriction effect of phenylephrine.

Original languageEnglish
Pages (from-to)373-380
Number of pages8
JournalApplied Physiology, Nutrition and Metabolism
Volume44
Issue number4
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Plant Oils
Citrus
Blood Vessels
Nitrites
Phenylephrine
Diet
Vasoconstriction
Tunica Media
Nitroprusside
Vasodilation
Biological Availability
Acetylcholine
Sprague Dawley Rats
Aorta
Nitric Oxide
palm oil
Hypertension

Keywords

  • Citrus
  • Hypertension
  • Nitric oxide
  • Rutaceae
  • Vascular reactivity
  • Vegetable oil

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Nutrition and Dietetics
  • Physiology (medical)

Cite this

Effects of citrus leaf extract on aortic vascular reactivity in hypertensive rats fed repeatedly heated vegetable oil. / Siti, Hawa Nordin; Yusof, Kamisah; Mohamed, Suhaila; Jaarin, Kamsiah.

In: Applied Physiology, Nutrition and Metabolism, Vol. 44, No. 4, 01.01.2019, p. 373-380.

Research output: Contribution to journalArticle

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