Effects of calcium salts on bovine corneal stromal transparency

Bariah Mohd. Ali, D. J. O'Leary, J. W. Huff

Research output: Contribution to journalArticle

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Abstract

Purpose: Many factors (e.g. pH, ionic strength) may affect corneal stromal transparency at a given hydration (H). In this study we investigated the effects of calcium salts at constant pH on stromal transparency as a function of H. Methojj: Bovine corneal stromas were immersed in 0.154M NaCl (control), CaCl2, Ca(C2H3O2)2 or CaNCh (all at pH 7.4, lOmM MOPS buffer). Double beam spectrophotometry measured direct transmission of stromas between A, = 750 nm and 400 nm, 1 and 30 minutes after immersion. Slopes (m) of log spectral transmission/log thickness give λm. H was determined by weighing. Results: In NaCl, m changed from -3 to -2 as H increased. In all calcium salts scattering was greater than in NaCl; m was -2 after 1 minute and approached -1 after 30 minutes. The change was less for Ca(C2H3O2)2 than for other Ca2+ salts, despite the similar hydratioa. Time 1 minute 30 minutes Salts (0.154M) H±SD m±SD H±SD m±SD NaCl 3.58 ±0.10 -3.08 ±0.18 4.89±0.25 -2.21±0.17 CaCl2 3.46 ±0.12 -1.97 ±0.24 4.86 ±0.20 -1.28 ±0.13 Ca(C2H3O2)2 3.39 ±0.14 -2.18 ±0.37 4.38 ± 0.05 -1.82 ± 0.27 CaNO3 3.43 ± 0.10 -1.89 ± 0.24 4.69 ±0.23 -1.01 ±0.09 p< 0.05 vs NaCl at the same timepoint Conclusion: Calcium decreases stromal transparency at a given hydration; the change in scattering behaviour (m) is consistent with the formation of larger scattering centres, possibly calcium precipitates or crystals, inside the stroma. Slower transparency losses in Ca(C2H3O2)2 solution are possibly due to the bigger acetate anion or less dissociation of Ca2+ in the solution.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
Publication statusPublished - 1997
Externally publishedYes

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Salts
Calcium
Corneal Stroma
Spectrophotometry
Immersion
Osmolar Concentration
Anions
Buffers
Acetates

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Effects of calcium salts on bovine corneal stromal transparency. / Mohd. Ali, Bariah; O'Leary, D. J.; Huff, J. W.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 1997.

Research output: Contribution to journalArticle

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abstract = "Purpose: Many factors (e.g. pH, ionic strength) may affect corneal stromal transparency at a given hydration (H). In this study we investigated the effects of calcium salts at constant pH on stromal transparency as a function of H. Methojj: Bovine corneal stromas were immersed in 0.154M NaCl (control), CaCl2, Ca(C2H3O2)2 or CaNCh (all at pH 7.4, lOmM MOPS buffer). Double beam spectrophotometry measured direct transmission of stromas between A, = 750 nm and 400 nm, 1 and 30 minutes after immersion. Slopes (m) of log spectral transmission/log thickness give λm. H was determined by weighing. Results: In NaCl, m changed from -3 to -2 as H increased. In all calcium salts scattering was greater than in NaCl; m was -2 after 1 minute and approached -1 after 30 minutes. The change was less for Ca(C2H3O2)2 than for other Ca2+ salts, despite the similar hydratioa. Time 1 minute 30 minutes Salts (0.154M) H±SD m±SD H±SD m±SD NaCl 3.58 ±0.10 -3.08 ±0.18 4.89±0.25 -2.21±0.17 CaCl2 3.46 ±0.12 -1.97 ±0.24 4.86 ±0.20 -1.28 ±0.13 Ca(C2H3O2)2 3.39 ±0.14 -2.18 ±0.37 4.38 ± 0.05 -1.82 ± 0.27 CaNO3 3.43 ± 0.10 -1.89 ± 0.24 4.69 ±0.23 -1.01 ±0.09 p< 0.05 vs NaCl at the same timepoint Conclusion: Calcium decreases stromal transparency at a given hydration; the change in scattering behaviour (m) is consistent with the formation of larger scattering centres, possibly calcium precipitates or crystals, inside the stroma. Slower transparency losses in Ca(C2H3O2)2 solution are possibly due to the bigger acetate anion or less dissociation of Ca2+ in the solution.",
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AB - Purpose: Many factors (e.g. pH, ionic strength) may affect corneal stromal transparency at a given hydration (H). In this study we investigated the effects of calcium salts at constant pH on stromal transparency as a function of H. Methojj: Bovine corneal stromas were immersed in 0.154M NaCl (control), CaCl2, Ca(C2H3O2)2 or CaNCh (all at pH 7.4, lOmM MOPS buffer). Double beam spectrophotometry measured direct transmission of stromas between A, = 750 nm and 400 nm, 1 and 30 minutes after immersion. Slopes (m) of log spectral transmission/log thickness give λm. H was determined by weighing. Results: In NaCl, m changed from -3 to -2 as H increased. In all calcium salts scattering was greater than in NaCl; m was -2 after 1 minute and approached -1 after 30 minutes. The change was less for Ca(C2H3O2)2 than for other Ca2+ salts, despite the similar hydratioa. Time 1 minute 30 minutes Salts (0.154M) H±SD m±SD H±SD m±SD NaCl 3.58 ±0.10 -3.08 ±0.18 4.89±0.25 -2.21±0.17 CaCl2 3.46 ±0.12 -1.97 ±0.24 4.86 ±0.20 -1.28 ±0.13 Ca(C2H3O2)2 3.39 ±0.14 -2.18 ±0.37 4.38 ± 0.05 -1.82 ± 0.27 CaNO3 3.43 ± 0.10 -1.89 ± 0.24 4.69 ±0.23 -1.01 ±0.09 p< 0.05 vs NaCl at the same timepoint Conclusion: Calcium decreases stromal transparency at a given hydration; the change in scattering behaviour (m) is consistent with the formation of larger scattering centres, possibly calcium precipitates or crystals, inside the stroma. Slower transparency losses in Ca(C2H3O2)2 solution are possibly due to the bigger acetate anion or less dissociation of Ca2+ in the solution.

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