Kesan Ekstrak Akueus Rosel (Hibiscus sabdariffa Linn.) terhadap Sperma dan Testis Tikus Diadministrasi Nikotin

Translated title of the contribution: Effects of aqueous extract of roselle (hibiscus sabdariffa linn.) on sperm and testis in rat-administered Nicotine

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1 Citation (Scopus)

Abstract

The generation of free radicals from nicotine is associated with reproductive damage especially on sperms and testis. The usage of treatment based on herbs such as Hibiscus sabdariffa Linn. (HSE) is gradually increased due to the high content of natural antioxidant. Therefore, this study was aimed to investigate the effects of extract aqueus HSE on sperms and testis in rat-administered nicotine. A total of 21 male Sprague Dawley rats were divided into three groups which were the control, nicotine and nicotine + HSE group. The nicotine was injected via intraperitoneum at the dose of 0.6 mg/kg body weight, while the HSE was given at the dose of 100 mg/kg body weight, orally before the nicotine administration for 21 censequtive days. The findings showed significantly (p<0.05) increased of sperm count, motility and viability while significantly (p< 0.05) decreased of percentage of abnormal sperm morphology in the nicotine + HSE group compared with the nicotine group. Furthermore, the level of malondialdehyde (MDA) reduced significantly (p<0.05) and the level of glutathione (GSH) increased significantly (p<0.05) in the nicotine + HSE group compared with the nicotine group. Histology study showed the potential of HSE in protecting the testis morphological alteration induced by nicotine. In conclusion, supplementation of HSE has the potential to prevent sperm and testis damage induced by nicotine.

Original languageMalay
Pages (from-to)1611-1616
Number of pages6
JournalSains Malaysiana
Volume46
Issue number9
DOIs
Publication statusPublished - 1 Sep 2017

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Hibiscus sabdariffa
nicotine
testes
spermatozoa
rats
extracts
water
body weight
dosage
malondialdehyde
histology
herbs
glutathione

ASJC Scopus subject areas

  • General

Cite this

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title = "Kesan Ekstrak Akueus Rosel (Hibiscus sabdariffa Linn.) terhadap Sperma dan Testis Tikus Diadministrasi Nikotin",
abstract = "The generation of free radicals from nicotine is associated with reproductive damage especially on sperms and testis. The usage of treatment based on herbs such as Hibiscus sabdariffa Linn. (HSE) is gradually increased due to the high content of natural antioxidant. Therefore, this study was aimed to investigate the effects of extract aqueus HSE on sperms and testis in rat-administered nicotine. A total of 21 male Sprague Dawley rats were divided into three groups which were the control, nicotine and nicotine + HSE group. The nicotine was injected via intraperitoneum at the dose of 0.6 mg/kg body weight, while the HSE was given at the dose of 100 mg/kg body weight, orally before the nicotine administration for 21 censequtive days. The findings showed significantly (p<0.05) increased of sperm count, motility and viability while significantly (p< 0.05) decreased of percentage of abnormal sperm morphology in the nicotine + HSE group compared with the nicotine group. Furthermore, the level of malondialdehyde (MDA) reduced significantly (p<0.05) and the level of glutathione (GSH) increased significantly (p<0.05) in the nicotine + HSE group compared with the nicotine group. Histology study showed the potential of HSE in protecting the testis morphological alteration induced by nicotine. In conclusion, supplementation of HSE has the potential to prevent sperm and testis damage induced by nicotine.",
keywords = "Antioxidant, Cigarette, Oxidative Stress, Reproductive, Roselle",
author = "{Izatus Shima}, Taib and Budin, {Siti Balkis} and Ismail, {Maizatul Nadhirah} and Satirah Zainalabidin and Jamaludin Mohamed",
year = "2017",
month = "9",
day = "1",
doi = "10.17576/jsm-2017-4609-33",
language = "Malay",
volume = "46",
pages = "1611--1616",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "9",

}

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T1 - Kesan Ekstrak Akueus Rosel (Hibiscus sabdariffa Linn.) terhadap Sperma dan Testis Tikus Diadministrasi Nikotin

AU - Izatus Shima, Taib

AU - Budin, Siti Balkis

AU - Ismail, Maizatul Nadhirah

AU - Zainalabidin, Satirah

AU - Mohamed, Jamaludin

PY - 2017/9/1

Y1 - 2017/9/1

N2 - The generation of free radicals from nicotine is associated with reproductive damage especially on sperms and testis. The usage of treatment based on herbs such as Hibiscus sabdariffa Linn. (HSE) is gradually increased due to the high content of natural antioxidant. Therefore, this study was aimed to investigate the effects of extract aqueus HSE on sperms and testis in rat-administered nicotine. A total of 21 male Sprague Dawley rats were divided into three groups which were the control, nicotine and nicotine + HSE group. The nicotine was injected via intraperitoneum at the dose of 0.6 mg/kg body weight, while the HSE was given at the dose of 100 mg/kg body weight, orally before the nicotine administration for 21 censequtive days. The findings showed significantly (p<0.05) increased of sperm count, motility and viability while significantly (p< 0.05) decreased of percentage of abnormal sperm morphology in the nicotine + HSE group compared with the nicotine group. Furthermore, the level of malondialdehyde (MDA) reduced significantly (p<0.05) and the level of glutathione (GSH) increased significantly (p<0.05) in the nicotine + HSE group compared with the nicotine group. Histology study showed the potential of HSE in protecting the testis morphological alteration induced by nicotine. In conclusion, supplementation of HSE has the potential to prevent sperm and testis damage induced by nicotine.

AB - The generation of free radicals from nicotine is associated with reproductive damage especially on sperms and testis. The usage of treatment based on herbs such as Hibiscus sabdariffa Linn. (HSE) is gradually increased due to the high content of natural antioxidant. Therefore, this study was aimed to investigate the effects of extract aqueus HSE on sperms and testis in rat-administered nicotine. A total of 21 male Sprague Dawley rats were divided into three groups which were the control, nicotine and nicotine + HSE group. The nicotine was injected via intraperitoneum at the dose of 0.6 mg/kg body weight, while the HSE was given at the dose of 100 mg/kg body weight, orally before the nicotine administration for 21 censequtive days. The findings showed significantly (p<0.05) increased of sperm count, motility and viability while significantly (p< 0.05) decreased of percentage of abnormal sperm morphology in the nicotine + HSE group compared with the nicotine group. Furthermore, the level of malondialdehyde (MDA) reduced significantly (p<0.05) and the level of glutathione (GSH) increased significantly (p<0.05) in the nicotine + HSE group compared with the nicotine group. Histology study showed the potential of HSE in protecting the testis morphological alteration induced by nicotine. In conclusion, supplementation of HSE has the potential to prevent sperm and testis damage induced by nicotine.

KW - Antioxidant

KW - Cigarette

KW - Oxidative Stress

KW - Reproductive

KW - Roselle

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