Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

Affidah Sabran, Kumolosasi Msi Endang, Ibrahim Jantan

Research output: Contribution to journalArticle

Abstract

Recent studies suggest that annexin A1 (ANXA1) promotes apoptosis in cancerous cells. This study aims to investigate the effects of ANXA1 on apoptosis and cell cycle arrest in K562, Jurkat and U937 cells and peripheral blood mononu-clear cells (PBMC). Cells were treated with ANXA1 and cyclophosphamide prior to flow cytometry analysis for apoptosis and cell cycle arrest induction. At 2.5μM, ANXA1 induced significant apoptosis in K562 (p ≤ 0.001) and U937 (p ≤ 0.05) cells, with EC50 values of 3.6 and 3.8 μM, respectively. In Jurkat cells, induction was not significant (EC50, 17.0 μM). No significant apoptosis induction was observed in PBMC. ANXA1 caused cycle arrest in the G0/G1 phase in K562 and U937 cells with p ≤ 0.001 for both, and (p ≤ 0.01) for Jurkat cells. ANXA1 induced apoptosis and cycle arrest in the G0/G1 phase in K562 and U937 cells, causing only cell cycle arrest in Jurkat cells.

Original languageEnglish
Pages (from-to)75-86
Number of pages12
JournalActa Pharmaceutica
Volume69
Issue number1
DOIs
Publication statusPublished - 1 Mar 2019

Fingerprint

Annexins
Annexin A1
Cell Cycle Checkpoints
Jurkat Cells
Apoptosis
Cell Line
U937 Cells
K562 Cells
Cell Cycle Resting Phase
G1 Phase
Cyclophosphamide
Flow Cytometry

Keywords

  • annexin A1
  • apoptosis
  • cell cycle
  • flow cytometry
  • human leukemic cell lines

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines. / Sabran, Affidah; Endang, Kumolosasi Msi; Jantan, Ibrahim.

In: Acta Pharmaceutica, Vol. 69, No. 1, 01.03.2019, p. 75-86.

Research output: Contribution to journalArticle

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