Effects of Aging and Tocotrienol-Rich Fraction Supplementation on Brain Arginine Metabolism in Rats

Musalmah Mazlan, Hamizah Shahirah Hamezah, Nursiati Mohd Taridi, Yu Jing, Ping Liu, Hu Zhang, Wan Zurinah Wan Ngah, Mohd Hanafi Ahmad Damanhuri

Research output: Contribution to journalArticle

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Abstract

Accumulating evidence suggests that altered arginine metabolism is involved in the aging and neurodegenerative processes. This study sought to determine the effects of age and vitamin E supplementation in the form of tocotrienol-rich fraction (TRF) on brain arginine metabolism. Male Wistar rats at ages of 3 and 21 months were supplemented with TRF orally for 3 months prior to the dissection of tissue from five brain regions. The tissue concentrations of L-arginine and its nine downstream metabolites were quantified using high-performance liquid chromatography and liquid chromatography tandem mass spectrometry. We found age-related alterations in L-arginine metabolites in the chemical- and region-specific manners. Moreover, TRF supplementation reversed age-associated changes in arginine metabolites in the entorhinal cortex and cerebellum. Multiple regression analysis revealed a number of significant neurochemical-behavioral correlations, indicating the beneficial effects of TRF supplementation on memory and motor function.

Original languageEnglish
Article number6019796
JournalOxidative Medicine and Cellular Longevity
Volume2017
DOIs
Publication statusPublished - 1 Jan 2017

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Tocotrienols
Metabolism
Arginine
Rats
Brain
Aging of materials
Metabolites
Tissue
Dissection
Entorhinal Cortex
Liquid chromatography
High performance liquid chromatography
Tandem Mass Spectrometry
Vitamin E
Regression analysis
Liquid Chromatography
Cerebellum
Mass spectrometry
Wistar Rats
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Ageing
  • Cell Biology

Cite this

Effects of Aging and Tocotrienol-Rich Fraction Supplementation on Brain Arginine Metabolism in Rats. / Mazlan, Musalmah; Hamezah, Hamizah Shahirah; Taridi, Nursiati Mohd; Jing, Yu; Liu, Ping; Zhang, Hu; Wan Ngah, Wan Zurinah; Ahmad Damanhuri, Mohd Hanafi.

In: Oxidative Medicine and Cellular Longevity, Vol. 2017, 6019796, 01.01.2017.

Research output: Contribution to journalArticle

Mazlan, Musalmah ; Hamezah, Hamizah Shahirah ; Taridi, Nursiati Mohd ; Jing, Yu ; Liu, Ping ; Zhang, Hu ; Wan Ngah, Wan Zurinah ; Ahmad Damanhuri, Mohd Hanafi. / Effects of Aging and Tocotrienol-Rich Fraction Supplementation on Brain Arginine Metabolism in Rats. In: Oxidative Medicine and Cellular Longevity. 2017 ; Vol. 2017.
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AU - Wan Ngah, Wan Zurinah

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