Effects of 17α-ethinylestradiol on embryo survival, hatchability and larval deformities in African catfish, Clarias gariepinus

M. Nadirah, M. A.B. Tajuddin, M. Najiah, Mazlan Abd. Ghaffar, K. L. Lee

Research output: Contribution to journalArticle

Abstract

Aim : Synthetic estrogens, such as 17α-ethinylestradiol (EE2), are common in contraceptive pills, and EE2 levels may be present in various waterways. As such qualitative and quantitative assessment on the impact of EE2 on aquatic organisms are needed. The main objective of the study was to determine the effect of EE2 at the environmentally relevant concentrations on embryonic survival, hatchability and larval deformitiesinAfrican catfish(Clarias gariepinus)cultured under laboratory conditions. Methodology: Two healthy sexually mature males and two gravid female fish weighing 0.8 to 1.0 kg were selected based on the external morphological features. They were then subjected to induced breeding with ovaprim, given at 0.25 b.wt. and 0.5 ml kg-1 b.wt. for males and females, respectively. Eight hundred fertilized eggs (embryos) were randomly distributed into four groups with 100 eggs per groupin duplicate in respective concentrations of EE2: control (0 ng l-1), 25 ng l-1, 50 ng l-1 and 100 ng l-1, each in 1000 ml glass beakers. Percentage of normal embryonic survival and abnormalities were recorded prior to hatching at 30th hour post fertilization (hpf), while 20 post-hatch larvae were sampled randomly from each group to determine the numberoflarvae displaying morphological deformities. Results : At 30th hpf, only 62% of the eggs survived when incubated with 100 ng l-1 of EE2, while the highest survival was observed inthe control group (92%). The lowest hatching success rateof 55% was seen in the 100ngl-1 group, which was significantly lower from the embryos exposedto 0 or 25 ngl-1. Interpretation : The results suggest that EE2 at 100 ng l-1 affected the early life stages of C. gariepinus, thus,acloser monitoringon EE2 discharge into the aquatic environmentsis essential. 2018 Triveni Enterprises.

Original languageEnglish
Pages (from-to)931-935
Number of pages5
JournalJournal of Environmental Biology
Volume39
Issue number5
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Catfishes
Ethinyl Estradiol
Fertilization
Eggs
Embryonic Structures
Estradiol Congeners
Aquatic organisms
Hatches
Aquatic Organisms
Zygote
Weighing
Contraceptive Agents
Fish
Breeding
Glass
Larva
Fishes
Control Groups
Industry
ovaprim

Keywords

  • 17α-Ethinylestradiol
  • Clarias gariepinus
  • Embryonic development
  • Hatchability
  • Larval deformities

ASJC Scopus subject areas

  • Environmental Engineering
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Effects of 17α-ethinylestradiol on embryo survival, hatchability and larval deformities in African catfish, Clarias gariepinus. / Nadirah, M.; Tajuddin, M. A.B.; Najiah, M.; Abd. Ghaffar, Mazlan; Lee, K. L.

In: Journal of Environmental Biology, Vol. 39, No. 5, 01.01.2018, p. 931-935.

Research output: Contribution to journalArticle

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abstract = "Aim : Synthetic estrogens, such as 17α-ethinylestradiol (EE2), are common in contraceptive pills, and EE2 levels may be present in various waterways. As such qualitative and quantitative assessment on the impact of EE2 on aquatic organisms are needed. The main objective of the study was to determine the effect of EE2 at the environmentally relevant concentrations on embryonic survival, hatchability and larval deformitiesinAfrican catfish(Clarias gariepinus)cultured under laboratory conditions. Methodology: Two healthy sexually mature males and two gravid female fish weighing 0.8 to 1.0 kg were selected based on the external morphological features. They were then subjected to induced breeding with ovaprim, given at 0.25 b.wt. and 0.5 ml kg-1 b.wt. for males and females, respectively. Eight hundred fertilized eggs (embryos) were randomly distributed into four groups with 100 eggs per groupin duplicate in respective concentrations of EE2: control (0 ng l-1), 25 ng l-1, 50 ng l-1 and 100 ng l-1, each in 1000 ml glass beakers. Percentage of normal embryonic survival and abnormalities were recorded prior to hatching at 30th hour post fertilization (hpf), while 20 post-hatch larvae were sampled randomly from each group to determine the numberoflarvae displaying morphological deformities. Results : At 30th hpf, only 62{\%} of the eggs survived when incubated with 100 ng l-1 of EE2, while the highest survival was observed inthe control group (92{\%}). The lowest hatching success rateof 55{\%} was seen in the 100ngl-1 group, which was significantly lower from the embryos exposedto 0 or 25 ngl-1. Interpretation : The results suggest that EE2 at 100 ng l-1 affected the early life stages of C. gariepinus, thus,acloser monitoringon EE2 discharge into the aquatic environmentsis essential. 2018 Triveni Enterprises.",
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AU - Abd. Ghaffar, Mazlan

AU - Lee, K. L.

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AB - Aim : Synthetic estrogens, such as 17α-ethinylestradiol (EE2), are common in contraceptive pills, and EE2 levels may be present in various waterways. As such qualitative and quantitative assessment on the impact of EE2 on aquatic organisms are needed. The main objective of the study was to determine the effect of EE2 at the environmentally relevant concentrations on embryonic survival, hatchability and larval deformitiesinAfrican catfish(Clarias gariepinus)cultured under laboratory conditions. Methodology: Two healthy sexually mature males and two gravid female fish weighing 0.8 to 1.0 kg were selected based on the external morphological features. They were then subjected to induced breeding with ovaprim, given at 0.25 b.wt. and 0.5 ml kg-1 b.wt. for males and females, respectively. Eight hundred fertilized eggs (embryos) were randomly distributed into four groups with 100 eggs per groupin duplicate in respective concentrations of EE2: control (0 ng l-1), 25 ng l-1, 50 ng l-1 and 100 ng l-1, each in 1000 ml glass beakers. Percentage of normal embryonic survival and abnormalities were recorded prior to hatching at 30th hour post fertilization (hpf), while 20 post-hatch larvae were sampled randomly from each group to determine the numberoflarvae displaying morphological deformities. Results : At 30th hpf, only 62% of the eggs survived when incubated with 100 ng l-1 of EE2, while the highest survival was observed inthe control group (92%). The lowest hatching success rateof 55% was seen in the 100ngl-1 group, which was significantly lower from the embryos exposedto 0 or 25 ngl-1. Interpretation : The results suggest that EE2 at 100 ng l-1 affected the early life stages of C. gariepinus, thus,acloser monitoringon EE2 discharge into the aquatic environmentsis essential. 2018 Triveni Enterprises.

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