Effective cell seeding and three-dimensional cell culture for bone tissue engineering

Min Hwei Ng, Shiplu Roy Chowdhury, Mahboob Morshed, Kok Keong Tan, Guan Huat Tan, Mun Yee Phang, Bin Saim Aminuddin, Othman Fauziah, Ruszymah Idrus

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Tricalcium phosphate-hydroxyapatite (TCP/HA) composite has been proven in previous studies as an ideal scaffold for making tissue engineered bone construct. Successful delivery of a sufficient number of living cells onto the scaffold is equally important. This study aims to optimize two key steps in the generation of a three dimensional (3D) bone construct i.e., cell seeding onto the 3D scaffold, and 3D construct maturation. The efficiency of cell seeding onto a scaffold with or without the addition of fibrin via static versus dynamic seeding techniques was evaluated using MTS assay and electron microscopy. The cell seeded constructs were then subjected to osteogenic differentiation in vitro and were evaluated for cell viability and alkaline phosphatase production (marker of osteogenic differentiation) after 1 and 21 days. Our results showed that the addition of fibrin during cell seeding resulted in constructs with greater cell density and matrix production. There was no significant difference was observed in cell seeding efficiency when seeded statically or dynamically. Dynamic cultivation of the bone constructs provided greater support for cell viability and differentiation within the constructs compared to static cultivation. We conclude that static cell seeding with the addition of fibrin followed by dynamic construct cultivation is optimal for 3D bone construct generation on TCP/HA scaffold.

Original languageEnglish
Pages (from-to)573-578
Number of pages6
JournalJournal of Biomaterials and Tissue Engineering
Volume4
Issue number7
DOIs
Publication statusPublished - 2014

Fingerprint

Tissue Engineering
Tissue engineering
Cell culture
Scaffolds
Bone
Cell Culture Techniques
Fibrin
Bone and Bones
Cells
Durapatite
Hydroxyapatite
Tissue Scaffolds
Phosphates
Cell Survival
Cell Count
Electron microscopy
Phosphatases
Alkaline Phosphatase
Assays
Differentiation Antigens

Keywords

  • Bone Tissue Engineering
  • Cell Seeding
  • MTS Assay
  • Porous Ceramic

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)

Cite this

Effective cell seeding and three-dimensional cell culture for bone tissue engineering. / Ng, Min Hwei; Chowdhury, Shiplu Roy; Morshed, Mahboob; Keong Tan, Kok; Huat Tan, Guan; Yee Phang, Mun; Saim Aminuddin, Bin; Fauziah, Othman; Idrus, Ruszymah.

In: Journal of Biomaterials and Tissue Engineering, Vol. 4, No. 7, 2014, p. 573-578.

Research output: Contribution to journalArticle

Ng, Min Hwei ; Chowdhury, Shiplu Roy ; Morshed, Mahboob ; Keong Tan, Kok ; Huat Tan, Guan ; Yee Phang, Mun ; Saim Aminuddin, Bin ; Fauziah, Othman ; Idrus, Ruszymah. / Effective cell seeding and three-dimensional cell culture for bone tissue engineering. In: Journal of Biomaterials and Tissue Engineering. 2014 ; Vol. 4, No. 7. pp. 573-578.
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