Abstract
Tricalcium phosphate-hydroxyapatite (TCP/HA) composite has been proven in previous studies as an ideal scaffold for making tissue engineered bone construct. Successful delivery of a sufficient number of living cells onto the scaffold is equally important. This study aims to optimize two key steps in the generation of a three dimensional (3D) bone construct i.e., cell seeding onto the 3D scaffold, and 3D construct maturation. The efficiency of cell seeding onto a scaffold with or without the addition of fibrin via static versus dynamic seeding techniques was evaluated using MTS assay and electron microscopy. The cell seeded constructs were then subjected to osteogenic differentiation in vitro and were evaluated for cell viability and alkaline phosphatase production (marker of osteogenic differentiation) after 1 and 21 days. Our results showed that the addition of fibrin during cell seeding resulted in constructs with greater cell density and matrix production. There was no significant difference was observed in cell seeding efficiency when seeded statically or dynamically. Dynamic cultivation of the bone constructs provided greater support for cell viability and differentiation within the constructs compared to static cultivation. We conclude that static cell seeding with the addition of fibrin followed by dynamic construct cultivation is optimal for 3D bone construct generation on TCP/HA scaffold.
| Original language | English |
|---|---|
| Pages (from-to) | 573-578 |
| Number of pages | 6 |
| Journal | Journal of Biomaterials and Tissue Engineering |
| Volume | 4 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Bone Tissue Engineering
- Cell Seeding
- MTS Assay
- Porous Ceramic
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Medicine (miscellaneous)
Cite this
Effective cell seeding and three-dimensional cell culture for bone tissue engineering. / Ng, Min Hwei; Chowdhury, Shiplu Roy; Morshed, Mahboob; Keong Tan, Kok; Huat Tan, Guan; Yee Phang, Mun; Saim Aminuddin, Bin; Fauziah, Othman; Idrus, Ruszymah.
In: Journal of Biomaterials and Tissue Engineering, Vol. 4, No. 7, 2014, p. 573-578.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effective cell seeding and three-dimensional cell culture for bone tissue engineering
AU - Ng, Min Hwei
AU - Chowdhury, Shiplu Roy
AU - Morshed, Mahboob
AU - Keong Tan, Kok
AU - Huat Tan, Guan
AU - Yee Phang, Mun
AU - Saim Aminuddin, Bin
AU - Fauziah, Othman
AU - Idrus, Ruszymah
PY - 2014
Y1 - 2014
N2 - Tricalcium phosphate-hydroxyapatite (TCP/HA) composite has been proven in previous studies as an ideal scaffold for making tissue engineered bone construct. Successful delivery of a sufficient number of living cells onto the scaffold is equally important. This study aims to optimize two key steps in the generation of a three dimensional (3D) bone construct i.e., cell seeding onto the 3D scaffold, and 3D construct maturation. The efficiency of cell seeding onto a scaffold with or without the addition of fibrin via static versus dynamic seeding techniques was evaluated using MTS assay and electron microscopy. The cell seeded constructs were then subjected to osteogenic differentiation in vitro and were evaluated for cell viability and alkaline phosphatase production (marker of osteogenic differentiation) after 1 and 21 days. Our results showed that the addition of fibrin during cell seeding resulted in constructs with greater cell density and matrix production. There was no significant difference was observed in cell seeding efficiency when seeded statically or dynamically. Dynamic cultivation of the bone constructs provided greater support for cell viability and differentiation within the constructs compared to static cultivation. We conclude that static cell seeding with the addition of fibrin followed by dynamic construct cultivation is optimal for 3D bone construct generation on TCP/HA scaffold.
AB - Tricalcium phosphate-hydroxyapatite (TCP/HA) composite has been proven in previous studies as an ideal scaffold for making tissue engineered bone construct. Successful delivery of a sufficient number of living cells onto the scaffold is equally important. This study aims to optimize two key steps in the generation of a three dimensional (3D) bone construct i.e., cell seeding onto the 3D scaffold, and 3D construct maturation. The efficiency of cell seeding onto a scaffold with or without the addition of fibrin via static versus dynamic seeding techniques was evaluated using MTS assay and electron microscopy. The cell seeded constructs were then subjected to osteogenic differentiation in vitro and were evaluated for cell viability and alkaline phosphatase production (marker of osteogenic differentiation) after 1 and 21 days. Our results showed that the addition of fibrin during cell seeding resulted in constructs with greater cell density and matrix production. There was no significant difference was observed in cell seeding efficiency when seeded statically or dynamically. Dynamic cultivation of the bone constructs provided greater support for cell viability and differentiation within the constructs compared to static cultivation. We conclude that static cell seeding with the addition of fibrin followed by dynamic construct cultivation is optimal for 3D bone construct generation on TCP/HA scaffold.
KW - Bone Tissue Engineering
KW - Cell Seeding
KW - MTS Assay
KW - Porous Ceramic
UR - http://www.scopus.com/inward/record.url?scp=84924707056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84924707056&partnerID=8YFLogxK
U2 - 10.1166/jbt.2014.1204
DO - 10.1166/jbt.2014.1204
M3 - Article
AN - SCOPUS:84924707056
VL - 4
SP - 573
EP - 578
JO - Journal of Biomaterials and Tissue Engineering
JF - Journal of Biomaterials and Tissue Engineering
SN - 2157-9083
IS - 7
ER -