Effect of various doses of palm vitamin E and tocopherol on aspirin-induced gastric lesions in rats

Kamsiah Jaarin, M. T. Gapor, M. I. Nafeeza, A. M. Fauzee

Research output: Contribution to journalArticle

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Abstract

This study examined the effects of vitamin E on the prevention of aspirin-induced gastric lesions. The study was divided into two phases. Phase 1 determined the effects of various doses of palm vitamin E on the factors affecting mucosal integrity. Thirty-two male rats of the Sprague-Dawley strain (200-250 g) were randomly divided into four groups. Group I was fed a normal diet (control), Groups II, III and IV were fed a diet supplemented with palm vitamin E in a dose of 60 mg/kg food, 100 mg/kg food and 150 mg/kg food, respectively. The rats were killed after 4 weeks of feeding for the determination of gastric malondialdehyde (MDA), acid and mucus content. There was a significant decrease in gastric MDA and gastric acid in all the palm vitamin E supplemented groups compared to control. However, these doses of palm vitamin E had no significant effect on gastric mucus. The phase 2 study determined the effect of multiple doses of palm vitamin E and tocopherol on the prevention of aspirin-induced gastric lesions. Fifty rats of the same weight and strain were randomized into seven groups. Group I was fed a normal diet; groups II to IV were fed with a palm vitamin E enriched diet in doses of 60 mg, 100 mg and 150 mg/kg food, respectively; groups V to VII were fed with a tocopherol-enriched diet in doses of 20 mg, 30 mg and 50 mg/kg food, respectively. After 4 weeks of feeding with the respective diets the rats were challenged with a single intra-gastric dose of 400 mg/kg body weight aspirin suspended in propylene glycol. The rats were killed 6 h post-aspirin exposure for the determination of gastric lesion index and gastric parameters as mentioned in the phase I study. The gastric lesions index was significantly lower in all the vitamin E groups compared to control. The lowest ulcer index was observed in the groups that received 100 mg of palm vitamin E and 30 mg tocopherol in the diet. However, there was no significant difference in ulcer indices between palm vitamin E and tocopherol-treated groups. The lower ulcer index was only accompanied by lower gastric MDA content. We conclude that both palm vitamin E in doses of 60 mg, 100 mg and 150 mg/kg food as well as tocopherol in doses of 20 mg, 30 mg and 50 mg/kg food are equally effective in preventing aspirin-induced gastric lesions. The most probable mechanism is through their ability in limiting the lipid peroxidation that is involved in aspirin-induced gastric lesions.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalInternational Journal of Experimental Pathology
Volume83
Issue number6
DOIs
Publication statusPublished - Dec 2002

Fingerprint

Tocopherols
Vitamin E
Aspirin
Stomach
Diet
Food
Malondialdehyde
Ulcer
Gastric Acid
Mucus
Propylene Glycol
Gastrointestinal Contents
Lipid Peroxidation
Sprague Dawley Rats
Body Weight

Keywords

  • Aspirin
  • Dose
  • Gastric lesion
  • Palm vitamin E
  • Rat
  • Tocopherol

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Effect of various doses of palm vitamin E and tocopherol on aspirin-induced gastric lesions in rats. / Jaarin, Kamsiah; Gapor, M. T.; Nafeeza, M. I.; Fauzee, A. M.

In: International Journal of Experimental Pathology, Vol. 83, No. 6, 12.2002, p. 295-301.

Research output: Contribution to journalArticle

Jaarin, Kamsiah ; Gapor, M. T. ; Nafeeza, M. I. ; Fauzee, A. M. / Effect of various doses of palm vitamin E and tocopherol on aspirin-induced gastric lesions in rats. In: International Journal of Experimental Pathology. 2002 ; Vol. 83, No. 6. pp. 295-301.
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