Kesan suplementasi tokotrienol ke atas perubahan hormon dan katekolamin otak tikus aruhan stres

Translated title of the contribution: Effect of tocotrienol supplementation on hormones and catecholamines in the brain of rats exposed to stress

Research output: Contribution to journalArticle

Abstract

Stres merupakan sebarang gangguan, cabaran atau ancaman kepada seseorang individu yang merangsang perubahan respon fizikal, mental dan emosi. Hormon stres dan katekolamin dirembeskan di dalam otak sebagai respon terhadap stres. Kajian ini mengenal pasti kesan tokotrienol tulen dan vitamin E sawit ke atas perubahan hormon stres dan katekolamin pada tikus yang diaruhkan stres imobilisasi rendaman air. Sebanyak 32 ekor tikus Wistar jantan telah dibahagikan secara rawak kepada empat kumpulan kajian. Dua kumpulan kawalan; kumpulan tanpa stres (NS) dan kumpulan tanpa stres (CS) serta dua kumpulan rawatan yang diberikan tokotrienol (TTS) atau vitamin E sawit (TFS) secara oral paksaan pada dos 60 mg/kg berat badan selama 28 hari. Setelah tamat tempoh rawatan, tikus daripada kumpulan CS, TTS dan TFS telah didedahkan kepada stres imobilisasi rendaman air selama tiga setengan jam. Kemudian, otak tikus diambil untuk pengukuran hormon pelepasan kotikotrofin (CRH), hormon adenokortikotropik (ACTH), norepinefrin, dopamin dan serotonin. Keputusan kajian mendapati peningkatan kandungan CRH, ACTH, norepinefrin dan dopamine di dalam otak tikus yang diaruh stres berbanding kumpulan kawalan tanpa stres. Kandungan serotonin pula menurun akibat stres. Walau bagaimanapun, tikus yang diberi suplementasi tokotrienol dan vitamin E sawit menunjukkan penurunan signifikan kandungan CRH, ACTH, norepinefrin dan dopamin serta peningkatan kandungan dan serotonin sehingga hampir ke aras normal. Tiada perbezaan antara pemberian tokotrienol dan vitamin E sawit. Sebagai kesimpulan, tokotrienol dan vitamin E sawit berkesan dalam mengawal perubahan hormon stres serta katekolamin pada otak tikus yang teraruh stres.

Stress includes any disturbances, challenges or threats to an individual, which stimulates physical, mental and emotional responses. Stress hormones and catecholamines are released from the brain in response to stress. Antioxidant properties of vitamin E had been proven to help in reducing stress. This study was designed to investigate the effects of tocotrienol and palm vitamin E supplementation on stress hormones and catecholamines in stress-induced rats. Thirty two male Wistar rats were randomly assigned into four groups, consisted of two control groups (NS and CS) which were fed with a commercially prepared normal rat diet while two treatment groups (TTS and TFS) were given tocotrienol or palm vitamin E orally in the dose of 60 mg/kg body weight. After 28 days of treatment; CS, TTS and TFS rats were subjected to water-immersion restraint stress for three and a half hours once. The rats were killed and their brain was taken to determine stress hormones and catecholamines levels. The findings showed that stressed (CS) rats had a significantly higher levels of CRH, ACTH, norepinephrine and dopamine and lower level of serotonin in the brain. Interestingly, supplementation with both tocotrienol and palm vitamin E were able to significantly reduced the CRH, ACTH, norepinephrine and dopamine levels. No difference was observed when comparing the effects of tocotrienol with palm vitamin E. In conclusion, tocotrienol and palm vitamin E are capable in reducing changes to the stress hormones and catecholamines in stress-induced rats.

Original languageMalay
Pages (from-to)2411-2419
Number of pages9
JournalSains Malaysiana
Volume47
Issue number10
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Tocotrienols
Vitamin E
Catecholamines
Hormones
Brain
Adrenocorticotropic Hormone
Serotonin
Dopamine
Norepinephrine
Air
Immersion
Wistar Rats

ASJC Scopus subject areas

  • General

Cite this

Kesan suplementasi tokotrienol ke atas perubahan hormon dan katekolamin otak tikus aruhan stres. / Mohd Fahami, Nur Azlina; Ramli, Naddiah Syafiqah; Latif, Elda Surhaida.

In: Sains Malaysiana, Vol. 47, No. 10, 01.10.2018, p. 2411-2419.

Research output: Contribution to journalArticle

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abstract = "Stres merupakan sebarang gangguan, cabaran atau ancaman kepada seseorang individu yang merangsang perubahan respon fizikal, mental dan emosi. Hormon stres dan katekolamin dirembeskan di dalam otak sebagai respon terhadap stres. Kajian ini mengenal pasti kesan tokotrienol tulen dan vitamin E sawit ke atas perubahan hormon stres dan katekolamin pada tikus yang diaruhkan stres imobilisasi rendaman air. Sebanyak 32 ekor tikus Wistar jantan telah dibahagikan secara rawak kepada empat kumpulan kajian. Dua kumpulan kawalan; kumpulan tanpa stres (NS) dan kumpulan tanpa stres (CS) serta dua kumpulan rawatan yang diberikan tokotrienol (TTS) atau vitamin E sawit (TFS) secara oral paksaan pada dos 60 mg/kg berat badan selama 28 hari. Setelah tamat tempoh rawatan, tikus daripada kumpulan CS, TTS dan TFS telah didedahkan kepada stres imobilisasi rendaman air selama tiga setengan jam. Kemudian, otak tikus diambil untuk pengukuran hormon pelepasan kotikotrofin (CRH), hormon adenokortikotropik (ACTH), norepinefrin, dopamin dan serotonin. Keputusan kajian mendapati peningkatan kandungan CRH, ACTH, norepinefrin dan dopamine di dalam otak tikus yang diaruh stres berbanding kumpulan kawalan tanpa stres. Kandungan serotonin pula menurun akibat stres. Walau bagaimanapun, tikus yang diberi suplementasi tokotrienol dan vitamin E sawit menunjukkan penurunan signifikan kandungan CRH, ACTH, norepinefrin dan dopamin serta peningkatan kandungan dan serotonin sehingga hampir ke aras normal. Tiada perbezaan antara pemberian tokotrienol dan vitamin E sawit. Sebagai kesimpulan, tokotrienol dan vitamin E sawit berkesan dalam mengawal perubahan hormon stres serta katekolamin pada otak tikus yang teraruh stres.Stress includes any disturbances, challenges or threats to an individual, which stimulates physical, mental and emotional responses. Stress hormones and catecholamines are released from the brain in response to stress. Antioxidant properties of vitamin E had been proven to help in reducing stress. This study was designed to investigate the effects of tocotrienol and palm vitamin E supplementation on stress hormones and catecholamines in stress-induced rats. Thirty two male Wistar rats were randomly assigned into four groups, consisted of two control groups (NS and CS) which were fed with a commercially prepared normal rat diet while two treatment groups (TTS and TFS) were given tocotrienol or palm vitamin E orally in the dose of 60 mg/kg body weight. After 28 days of treatment; CS, TTS and TFS rats were subjected to water-immersion restraint stress for three and a half hours once. The rats were killed and their brain was taken to determine stress hormones and catecholamines levels. The findings showed that stressed (CS) rats had a significantly higher levels of CRH, ACTH, norepinephrine and dopamine and lower level of serotonin in the brain. Interestingly, supplementation with both tocotrienol and palm vitamin E were able to significantly reduced the CRH, ACTH, norepinephrine and dopamine levels. No difference was observed when comparing the effects of tocotrienol with palm vitamin E. In conclusion, tocotrienol and palm vitamin E are capable in reducing changes to the stress hormones and catecholamines in stress-induced rats.",
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T1 - Kesan suplementasi tokotrienol ke atas perubahan hormon dan katekolamin otak tikus aruhan stres

AU - Mohd Fahami, Nur Azlina

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N2 - Stres merupakan sebarang gangguan, cabaran atau ancaman kepada seseorang individu yang merangsang perubahan respon fizikal, mental dan emosi. Hormon stres dan katekolamin dirembeskan di dalam otak sebagai respon terhadap stres. Kajian ini mengenal pasti kesan tokotrienol tulen dan vitamin E sawit ke atas perubahan hormon stres dan katekolamin pada tikus yang diaruhkan stres imobilisasi rendaman air. Sebanyak 32 ekor tikus Wistar jantan telah dibahagikan secara rawak kepada empat kumpulan kajian. Dua kumpulan kawalan; kumpulan tanpa stres (NS) dan kumpulan tanpa stres (CS) serta dua kumpulan rawatan yang diberikan tokotrienol (TTS) atau vitamin E sawit (TFS) secara oral paksaan pada dos 60 mg/kg berat badan selama 28 hari. Setelah tamat tempoh rawatan, tikus daripada kumpulan CS, TTS dan TFS telah didedahkan kepada stres imobilisasi rendaman air selama tiga setengan jam. Kemudian, otak tikus diambil untuk pengukuran hormon pelepasan kotikotrofin (CRH), hormon adenokortikotropik (ACTH), norepinefrin, dopamin dan serotonin. Keputusan kajian mendapati peningkatan kandungan CRH, ACTH, norepinefrin dan dopamine di dalam otak tikus yang diaruh stres berbanding kumpulan kawalan tanpa stres. Kandungan serotonin pula menurun akibat stres. Walau bagaimanapun, tikus yang diberi suplementasi tokotrienol dan vitamin E sawit menunjukkan penurunan signifikan kandungan CRH, ACTH, norepinefrin dan dopamin serta peningkatan kandungan dan serotonin sehingga hampir ke aras normal. Tiada perbezaan antara pemberian tokotrienol dan vitamin E sawit. Sebagai kesimpulan, tokotrienol dan vitamin E sawit berkesan dalam mengawal perubahan hormon stres serta katekolamin pada otak tikus yang teraruh stres.Stress includes any disturbances, challenges or threats to an individual, which stimulates physical, mental and emotional responses. Stress hormones and catecholamines are released from the brain in response to stress. Antioxidant properties of vitamin E had been proven to help in reducing stress. This study was designed to investigate the effects of tocotrienol and palm vitamin E supplementation on stress hormones and catecholamines in stress-induced rats. Thirty two male Wistar rats were randomly assigned into four groups, consisted of two control groups (NS and CS) which were fed with a commercially prepared normal rat diet while two treatment groups (TTS and TFS) were given tocotrienol or palm vitamin E orally in the dose of 60 mg/kg body weight. After 28 days of treatment; CS, TTS and TFS rats were subjected to water-immersion restraint stress for three and a half hours once. The rats were killed and their brain was taken to determine stress hormones and catecholamines levels. The findings showed that stressed (CS) rats had a significantly higher levels of CRH, ACTH, norepinephrine and dopamine and lower level of serotonin in the brain. Interestingly, supplementation with both tocotrienol and palm vitamin E were able to significantly reduced the CRH, ACTH, norepinephrine and dopamine levels. No difference was observed when comparing the effects of tocotrienol with palm vitamin E. In conclusion, tocotrienol and palm vitamin E are capable in reducing changes to the stress hormones and catecholamines in stress-induced rats.

AB - Stres merupakan sebarang gangguan, cabaran atau ancaman kepada seseorang individu yang merangsang perubahan respon fizikal, mental dan emosi. Hormon stres dan katekolamin dirembeskan di dalam otak sebagai respon terhadap stres. Kajian ini mengenal pasti kesan tokotrienol tulen dan vitamin E sawit ke atas perubahan hormon stres dan katekolamin pada tikus yang diaruhkan stres imobilisasi rendaman air. Sebanyak 32 ekor tikus Wistar jantan telah dibahagikan secara rawak kepada empat kumpulan kajian. Dua kumpulan kawalan; kumpulan tanpa stres (NS) dan kumpulan tanpa stres (CS) serta dua kumpulan rawatan yang diberikan tokotrienol (TTS) atau vitamin E sawit (TFS) secara oral paksaan pada dos 60 mg/kg berat badan selama 28 hari. Setelah tamat tempoh rawatan, tikus daripada kumpulan CS, TTS dan TFS telah didedahkan kepada stres imobilisasi rendaman air selama tiga setengan jam. Kemudian, otak tikus diambil untuk pengukuran hormon pelepasan kotikotrofin (CRH), hormon adenokortikotropik (ACTH), norepinefrin, dopamin dan serotonin. Keputusan kajian mendapati peningkatan kandungan CRH, ACTH, norepinefrin dan dopamine di dalam otak tikus yang diaruh stres berbanding kumpulan kawalan tanpa stres. Kandungan serotonin pula menurun akibat stres. Walau bagaimanapun, tikus yang diberi suplementasi tokotrienol dan vitamin E sawit menunjukkan penurunan signifikan kandungan CRH, ACTH, norepinefrin dan dopamin serta peningkatan kandungan dan serotonin sehingga hampir ke aras normal. Tiada perbezaan antara pemberian tokotrienol dan vitamin E sawit. Sebagai kesimpulan, tokotrienol dan vitamin E sawit berkesan dalam mengawal perubahan hormon stres serta katekolamin pada otak tikus yang teraruh stres.Stress includes any disturbances, challenges or threats to an individual, which stimulates physical, mental and emotional responses. Stress hormones and catecholamines are released from the brain in response to stress. Antioxidant properties of vitamin E had been proven to help in reducing stress. This study was designed to investigate the effects of tocotrienol and palm vitamin E supplementation on stress hormones and catecholamines in stress-induced rats. Thirty two male Wistar rats were randomly assigned into four groups, consisted of two control groups (NS and CS) which were fed with a commercially prepared normal rat diet while two treatment groups (TTS and TFS) were given tocotrienol or palm vitamin E orally in the dose of 60 mg/kg body weight. After 28 days of treatment; CS, TTS and TFS rats were subjected to water-immersion restraint stress for three and a half hours once. The rats were killed and their brain was taken to determine stress hormones and catecholamines levels. The findings showed that stressed (CS) rats had a significantly higher levels of CRH, ACTH, norepinephrine and dopamine and lower level of serotonin in the brain. Interestingly, supplementation with both tocotrienol and palm vitamin E were able to significantly reduced the CRH, ACTH, norepinephrine and dopamine levels. No difference was observed when comparing the effects of tocotrienol with palm vitamin E. In conclusion, tocotrienol and palm vitamin E are capable in reducing changes to the stress hormones and catecholamines in stress-induced rats.

KW - Brain

KW - Catecholamines

KW - Palm Vitamin E

KW - Restraint stress

KW - Stress hormones

KW - Tocotrienol

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