Effect of the interaction of graphene oxide nanoparticles on a biological model cell membrane

Norsuriani Che Hashim, Sahlil Miraz Mohamed Rafie, Nurul Syahidah Ismail, Darman Nordin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Understanding the interaction of graphene oxide (GO) with a lipid membrane is important for the development of tissue engineering and to advance graphene-based biology. In an effort to understand the GO-lipid membrane interaction, appropriate characterisation of GO structure was determined by using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and a field emission scanning electron microscope (FESEM). In this study, the lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were used to produce a lipid vesicle with a conventional gentle hydration method and observed under transmission electron microscopy (TEM). Lipid vesicle-GO interactions were also investigated using dynamic light scattering (DLS) (Malvern Zeta) and TEM by focusing on the effect of the surface charge interactions and localisation of GO on the surface of the vesicle membrane. It was observed that the surface charge of the vesicles increased as the GO nanoparticle concentration increased, but for the low saturation lipid the surface charge remained high as the nanoparticle concentration increased. The localisation and positioning of the GO nanoparticles in the lipid vesicles were confirmed with TEM analysis.

Original languageEnglish
Article numberem60
JournalEurasian Journal of Analytical Chemistry
Volume13
Issue number5
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Biological Models
Graphite
Cell membranes
Nanoparticles
Oxides
Graphene
Cell Membrane
Lipids
Surface charge
Transmission Electron Microscopy
Membrane Lipids
Transmission electron microscopy
Dynamic light scattering
Fourier Transform Infrared Spectroscopy
Tissue Engineering
Tissue engineering
X-Ray Diffraction
Hydration
Field emission
Fourier transform infrared spectroscopy

Keywords

  • Distribution
  • Graphene oxide (GO)
  • Interaction lipid-GO
  • Lipid vesicles
  • Membrane permeability

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Spectroscopy
  • Electrochemistry

Cite this

Effect of the interaction of graphene oxide nanoparticles on a biological model cell membrane. / Hashim, Norsuriani Che; Rafie, Sahlil Miraz Mohamed; Ismail, Nurul Syahidah; Nordin, Darman.

In: Eurasian Journal of Analytical Chemistry, Vol. 13, No. 5, em60, 01.01.2018.

Research output: Contribution to journalArticle

Hashim, Norsuriani Che ; Rafie, Sahlil Miraz Mohamed ; Ismail, Nurul Syahidah ; Nordin, Darman. / Effect of the interaction of graphene oxide nanoparticles on a biological model cell membrane. In: Eurasian Journal of Analytical Chemistry. 2018 ; Vol. 13, No. 5.
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