Effect of the combination of gelam honey and ginger on oxidative stress and metabolic profile in streptozotocin-induced diabetic sprague-dawley rats

Nur Fathiah Abdul Sani, Levin Kesu Belani, Chong Pui Sin, Siti Nor Amilah Abdul Rahman, Srijit Das, Thent Zar Chi, Suzana Makpol, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Diabetic complications occur as a result of increased reactive oxygen species (ROS) due to long term hyperglycaemia. Honey and ginger have been shown to exhibit antioxidant activity which can scavenge ROS. The main aim of this study was to evaluate the antioxidant and antidiabetic effects of gelam honey, ginger, and their combination. Sprague-Dawley rats were divided into 2 major groups which consisted of diabetic and nondiabetic rats. Diabetes was induced with streptozotocin intramuscularly (55 mg/kg body weight). Each group was further divided into 4 smaller groups according to the supplements administered: distilled water, honey (2 g/kg body weight), ginger (60 mg/kg body weight), and honey + ginger. Body weight and glucose levels were recorded weekly, while blood from the orbital sinus was obtained after 3 weeks of supplementation for the estimation of metabolic profile: glucose, triglyceride (TG), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH): oxidized glutathione (GSSG), and malondialdehyde (MDA). The combination of gelam honey and ginger did not show hypoglycaemic potential; however, the combination treatment reduced significantly (P < 0.05) SOD and CAT activities as well as MDA level, while GSH level and GSH/GSSG ratio were significantly elevated (P < 0.05) in STZ-induced diabetic rats compared to diabetic control rats.

Original languageEnglish
Article number160695
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

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Ginger
Oxidative stress
Honey
Glutathione Disulfide
Metabolome
Streptozocin
Sprague Dawley Rats
Rats
Oxidative Stress
Malondialdehyde
Hypoglycemic Agents
Catalase
Superoxide Dismutase
Body Weight
Reactive Oxygen Species
Antioxidants
Rat control
Glucose
Medical problems
Glutathione Peroxidase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Effect of the combination of gelam honey and ginger on oxidative stress and metabolic profile in streptozotocin-induced diabetic sprague-dawley rats. / Abdul Sani, Nur Fathiah; Belani, Levin Kesu; Pui Sin, Chong; Abdul Rahman, Siti Nor Amilah; Das, Srijit; Zar Chi, Thent; Makpol, Suzana; Mohd Yusof, Yasmin Anum.

In: BioMed Research International, Vol. 2014, 160695, 2014.

Research output: Contribution to journalArticle

Abdul Sani, Nur Fathiah ; Belani, Levin Kesu ; Pui Sin, Chong ; Abdul Rahman, Siti Nor Amilah ; Das, Srijit ; Zar Chi, Thent ; Makpol, Suzana ; Mohd Yusof, Yasmin Anum. / Effect of the combination of gelam honey and ginger on oxidative stress and metabolic profile in streptozotocin-induced diabetic sprague-dawley rats. In: BioMed Research International. 2014 ; Vol. 2014.
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