Effect of steroid hormones on muscarinic receptors of bronchial smooth muscle

Nabishah Mohamad, P. B. Morat, B. A. Kadir, B. A K Khalid

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

1. 1. Glucocorticosteroid may relieve bronchospasm by mediating changes in the muscarinic receptor concentration and/or its affinity. 2. 2. Cholinergic muscarinic receptors were determined by using Scatchard's plots from radioligand binding assays of 0.13-3.2 nM [3H]quinuclidinyl benzylate binding to the membrane fraction of bronchial smooth muscle (BSM). 3. 3. The concentration of muscarinic receptor in BSM of normal rat was 57 ± 3 fmol/mg protein and the dissociation constant was 0.07 ± 0.02 nM. Dexamethasone and corticosterone reduced muscarinic receptor concentration to 50-60% of basal with no changes in receptor affinity. No changes were found in rat treated with deoxycorticosterone. 4. 4. These findings suggest that glucocorticoids but not mineralocorticoid relieve bronchospasm at least partly by reducing the cholinergic hypersensitivity.

Original languageEnglish
Pages (from-to)389-392
Number of pages4
JournalGeneral Pharmacology
Volume22
Issue number2
DOIs
Publication statusPublished - 1991

Fingerprint

Muscarinic Receptors
Smooth Muscle
Steroids
Hormones
Bronchial Spasm
Desoxycorticosterone
Mineralocorticoids
Radioligand Assay
Cholinergic Receptors
Corticosterone
Cholinergic Agents
Dexamethasone
Glucocorticoids
Hypersensitivity
Membranes
Proteins

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effect of steroid hormones on muscarinic receptors of bronchial smooth muscle. / Mohamad, Nabishah; Morat, P. B.; Kadir, B. A.; Khalid, B. A K.

In: General Pharmacology, Vol. 22, No. 2, 1991, p. 389-392.

Research output: Contribution to journalArticle

Mohamad, Nabishah ; Morat, P. B. ; Kadir, B. A. ; Khalid, B. A K. / Effect of steroid hormones on muscarinic receptors of bronchial smooth muscle. In: General Pharmacology. 1991 ; Vol. 22, No. 2. pp. 389-392.
@article{0822a1b1f5cd4e56a6c07c475a539775,
title = "Effect of steroid hormones on muscarinic receptors of bronchial smooth muscle",
abstract = "1. 1. Glucocorticosteroid may relieve bronchospasm by mediating changes in the muscarinic receptor concentration and/or its affinity. 2. 2. Cholinergic muscarinic receptors were determined by using Scatchard's plots from radioligand binding assays of 0.13-3.2 nM [3H]quinuclidinyl benzylate binding to the membrane fraction of bronchial smooth muscle (BSM). 3. 3. The concentration of muscarinic receptor in BSM of normal rat was 57 ± 3 fmol/mg protein and the dissociation constant was 0.07 ± 0.02 nM. Dexamethasone and corticosterone reduced muscarinic receptor concentration to 50-60{\%} of basal with no changes in receptor affinity. No changes were found in rat treated with deoxycorticosterone. 4. 4. These findings suggest that glucocorticoids but not mineralocorticoid relieve bronchospasm at least partly by reducing the cholinergic hypersensitivity.",
author = "Nabishah Mohamad and Morat, {P. B.} and Kadir, {B. A.} and Khalid, {B. A K}",
year = "1991",
doi = "10.1016/0306-3623(91)90469-M",
language = "English",
volume = "22",
pages = "389--392",
journal = "Vascular Pharmacology",
issn = "1537-1891",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Effect of steroid hormones on muscarinic receptors of bronchial smooth muscle

AU - Mohamad, Nabishah

AU - Morat, P. B.

AU - Kadir, B. A.

AU - Khalid, B. A K

PY - 1991

Y1 - 1991

N2 - 1. 1. Glucocorticosteroid may relieve bronchospasm by mediating changes in the muscarinic receptor concentration and/or its affinity. 2. 2. Cholinergic muscarinic receptors were determined by using Scatchard's plots from radioligand binding assays of 0.13-3.2 nM [3H]quinuclidinyl benzylate binding to the membrane fraction of bronchial smooth muscle (BSM). 3. 3. The concentration of muscarinic receptor in BSM of normal rat was 57 ± 3 fmol/mg protein and the dissociation constant was 0.07 ± 0.02 nM. Dexamethasone and corticosterone reduced muscarinic receptor concentration to 50-60% of basal with no changes in receptor affinity. No changes were found in rat treated with deoxycorticosterone. 4. 4. These findings suggest that glucocorticoids but not mineralocorticoid relieve bronchospasm at least partly by reducing the cholinergic hypersensitivity.

AB - 1. 1. Glucocorticosteroid may relieve bronchospasm by mediating changes in the muscarinic receptor concentration and/or its affinity. 2. 2. Cholinergic muscarinic receptors were determined by using Scatchard's plots from radioligand binding assays of 0.13-3.2 nM [3H]quinuclidinyl benzylate binding to the membrane fraction of bronchial smooth muscle (BSM). 3. 3. The concentration of muscarinic receptor in BSM of normal rat was 57 ± 3 fmol/mg protein and the dissociation constant was 0.07 ± 0.02 nM. Dexamethasone and corticosterone reduced muscarinic receptor concentration to 50-60% of basal with no changes in receptor affinity. No changes were found in rat treated with deoxycorticosterone. 4. 4. These findings suggest that glucocorticoids but not mineralocorticoid relieve bronchospasm at least partly by reducing the cholinergic hypersensitivity.

UR - http://www.scopus.com/inward/record.url?scp=0025977315&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025977315&partnerID=8YFLogxK

U2 - 10.1016/0306-3623(91)90469-M

DO - 10.1016/0306-3623(91)90469-M

M3 - Article

C2 - 1647349

AN - SCOPUS:0025977315

VL - 22

SP - 389

EP - 392

JO - Vascular Pharmacology

JF - Vascular Pharmacology

SN - 1537-1891

IS - 2

ER -