Effect of some environmental parameters on hydrogen production using C. acetobutylicum

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Abstract

The aim of this study was to investigate the influence of some environmental factors on bacterial metabolism. Fermentative hydrogen production by C. acetobutylicum, using glucose as the substrate. The effect of initial pH (4-8), inoculum size (1-20% (v/v)) and glucose concentration (1-30 g L -1) on hydrogen production were studied. The optimum cultivation temperature for hydrogen production was at 30°C. The results show that substrate concentration and inoculum size resulted in hydrogen yield (Y P/S) of 391 mL g-1 glucose utilized with maximum hydrogen productivity of 77.5 mL/L/h. Higher substrate concentration or inoculum size adversely affects hydrogen production, which decreases hydrogen yield by 15% to 334 mL g-1 glucose utilized when 30% (v/v) inoculum size was used. The use of 30 g L-1 substrate concentration resulted in a 75% decrease to 97 mL g-1 glucose supplied. Concluded that proper Xo/So enhanced the hydrogen production.

Original languageEnglish
Pages (from-to)2073-2082
Number of pages10
JournalPakistan Journal of Biological Sciences
Volume11
Issue number17
DOIs
Publication statusPublished - 2008

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hydrogen production
inoculum
glucose
hydrogen
environmental factors
metabolism
temperature

Keywords

  • Biohydrogen
  • Glucose
  • Inoculum size
  • pH

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

@article{6e204b90f2d941f8a445bb33e5a73468,
title = "Effect of some environmental parameters on hydrogen production using C. acetobutylicum",
abstract = "The aim of this study was to investigate the influence of some environmental factors on bacterial metabolism. Fermentative hydrogen production by C. acetobutylicum, using glucose as the substrate. The effect of initial pH (4-8), inoculum size (1-20{\%} (v/v)) and glucose concentration (1-30 g L -1) on hydrogen production were studied. The optimum cultivation temperature for hydrogen production was at 30°C. The results show that substrate concentration and inoculum size resulted in hydrogen yield (Y P/S) of 391 mL g-1 glucose utilized with maximum hydrogen productivity of 77.5 mL/L/h. Higher substrate concentration or inoculum size adversely affects hydrogen production, which decreases hydrogen yield by 15{\%} to 334 mL g-1 glucose utilized when 30{\%} (v/v) inoculum size was used. The use of 30 g L-1 substrate concentration resulted in a 75{\%} decrease to 97 mL g-1 glucose supplied. Concluded that proper Xo/So enhanced the hydrogen production.",
keywords = "Biohydrogen, Glucose, Inoculum size, pH",
author = "Hisham Alshiyab and Kalil, {Mohd. Sahaid} and {Abdul Hamid}, Aidil and {Wan Yusoff}, {Wan Mohtar}",
year = "2008",
doi = "10.3923/pjbs.2008.2073.2082",
language = "English",
volume = "11",
pages = "2073--2082",
journal = "Pakistan Journal of Biological Sciences",
issn = "1028-8880",
publisher = "Asian Network for Scientific Information",
number = "17",

}

TY - JOUR

T1 - Effect of some environmental parameters on hydrogen production using C. acetobutylicum

AU - Alshiyab, Hisham

AU - Kalil, Mohd. Sahaid

AU - Abdul Hamid, Aidil

AU - Wan Yusoff, Wan Mohtar

PY - 2008

Y1 - 2008

N2 - The aim of this study was to investigate the influence of some environmental factors on bacterial metabolism. Fermentative hydrogen production by C. acetobutylicum, using glucose as the substrate. The effect of initial pH (4-8), inoculum size (1-20% (v/v)) and glucose concentration (1-30 g L -1) on hydrogen production were studied. The optimum cultivation temperature for hydrogen production was at 30°C. The results show that substrate concentration and inoculum size resulted in hydrogen yield (Y P/S) of 391 mL g-1 glucose utilized with maximum hydrogen productivity of 77.5 mL/L/h. Higher substrate concentration or inoculum size adversely affects hydrogen production, which decreases hydrogen yield by 15% to 334 mL g-1 glucose utilized when 30% (v/v) inoculum size was used. The use of 30 g L-1 substrate concentration resulted in a 75% decrease to 97 mL g-1 glucose supplied. Concluded that proper Xo/So enhanced the hydrogen production.

AB - The aim of this study was to investigate the influence of some environmental factors on bacterial metabolism. Fermentative hydrogen production by C. acetobutylicum, using glucose as the substrate. The effect of initial pH (4-8), inoculum size (1-20% (v/v)) and glucose concentration (1-30 g L -1) on hydrogen production were studied. The optimum cultivation temperature for hydrogen production was at 30°C. The results show that substrate concentration and inoculum size resulted in hydrogen yield (Y P/S) of 391 mL g-1 glucose utilized with maximum hydrogen productivity of 77.5 mL/L/h. Higher substrate concentration or inoculum size adversely affects hydrogen production, which decreases hydrogen yield by 15% to 334 mL g-1 glucose utilized when 30% (v/v) inoculum size was used. The use of 30 g L-1 substrate concentration resulted in a 75% decrease to 97 mL g-1 glucose supplied. Concluded that proper Xo/So enhanced the hydrogen production.

KW - Biohydrogen

KW - Glucose

KW - Inoculum size

KW - pH

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U2 - 10.3923/pjbs.2008.2073.2082

DO - 10.3923/pjbs.2008.2073.2082

M3 - Article

VL - 11

SP - 2073

EP - 2082

JO - Pakistan Journal of Biological Sciences

JF - Pakistan Journal of Biological Sciences

SN - 1028-8880

IS - 17

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