Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum

Ahmed A. Mohammedelnour, M. E.S. Mirghani, Nasser A. Kabbashi, Md Z. Alam, Khalid H. Musa, Aminah Abdullah

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees. APG is rich in soluble dietary fibers as well as organic compounds. In this study quantitation of the levels of total phenolics content (TPC) and antioxidant activities were conducted using ABTS and CUPRAC assays for APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50% and 100%. The antioxidant levels were evaluated by 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation deculturization and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. The solvent Methanol (50%) gave the best extraction ratio for APG presented by highest (TPC 60.78 mg GAE/100g of DW, CUPRAC 34.65 mg TE/100g DW, and, ABTS about 37.65 mg TE/100g DW respectively), followed by ethanol 50% extract. On the other hand, pure methanol showed the lowest TPC 5.33 mg GAE/100g of DW, ABTS 10.9 mg TE/100g DW, and CUPRAC 7.80 mg TE/100g DW, values respectively. Therefore, the variation in the antioxidant capacity of extracts was possibly due to the difference of polarity, immiscibility and the nature of the APG compounds extracted using various solvents. The higher content of antioxidant activity in APG shall be useful to human health if it is properly utilized.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalInternational Food Research Journal
Volume24
Publication statusPublished - 1 Jan 2017

Fingerprint

Gum Arabic
Acacia
Antioxidants
antioxidant activity
Methanol
methanol
Ethanol
ethanol
Sulfonic Acids
antioxidants
Dietary Fiber
Exudates and Transudates
Acetone
sulfonic acid
extracts
Cations
organic compounds
branches
acetone
Iron

Keywords

  • Acacia polyacantha gum antioxidant activity
  • Gum Arabic
  • Solvent extraction
  • Total phenolic content

ASJC Scopus subject areas

  • Food Science

Cite this

Mohammedelnour, A. A., Mirghani, M. E. S., Kabbashi, N. A., Alam, M. Z., Musa, K. H., & Abdullah, A. (2017). Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum. International Food Research Journal, 24, 369-377.

Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum. / Mohammedelnour, Ahmed A.; Mirghani, M. E.S.; Kabbashi, Nasser A.; Alam, Md Z.; Musa, Khalid H.; Abdullah, Aminah.

In: International Food Research Journal, Vol. 24, 01.01.2017, p. 369-377.

Research output: Contribution to journalArticle

Mohammedelnour, AA, Mirghani, MES, Kabbashi, NA, Alam, MZ, Musa, KH & Abdullah, A 2017, 'Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum', International Food Research Journal, vol. 24, pp. 369-377.
Mohammedelnour AA, Mirghani MES, Kabbashi NA, Alam MZ, Musa KH, Abdullah A. Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum. International Food Research Journal. 2017 Jan 1;24:369-377.
Mohammedelnour, Ahmed A. ; Mirghani, M. E.S. ; Kabbashi, Nasser A. ; Alam, Md Z. ; Musa, Khalid H. ; Abdullah, Aminah. / Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum. In: International Food Research Journal. 2017 ; Vol. 24. pp. 369-377.
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abstract = "Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees. APG is rich in soluble dietary fibers as well as organic compounds. In this study quantitation of the levels of total phenolics content (TPC) and antioxidant activities were conducted using ABTS and CUPRAC assays for APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50{\%} and 100{\%}. The antioxidant levels were evaluated by 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation deculturization and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. The solvent Methanol (50{\%}) gave the best extraction ratio for APG presented by highest (TPC 60.78 mg GAE/100g of DW, CUPRAC 34.65 mg TE/100g DW, and, ABTS about 37.65 mg TE/100g DW respectively), followed by ethanol 50{\%} extract. On the other hand, pure methanol showed the lowest TPC 5.33 mg GAE/100g of DW, ABTS 10.9 mg TE/100g DW, and CUPRAC 7.80 mg TE/100g DW, values respectively. Therefore, the variation in the antioxidant capacity of extracts was possibly due to the difference of polarity, immiscibility and the nature of the APG compounds extracted using various solvents. The higher content of antioxidant activity in APG shall be useful to human health if it is properly utilized.",
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