Kesan psikostres terhadap kerosakan DNA dan ketaknormalan titisan sitoplasma sperma manusia

Translated title of the contribution: Effect of psychostress on DNA damage and cytoplasm droplet abnormality in human sperm

Norhamizan Hashim, Khairul Osman, Siti Fatimah Ibrahim, Rosliah Harun, Rafeah Pakri Mohamed

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

Idiopathic infertility has been correlated with psychostress in men. Nevertheless, direct relationship of low semen quality to psychostress still remains unclear. Hence, this study was conducted to evaluate the effect of psychostress on semen quality especially on the sperms DNA and cytoplasm droplet abnormality. In this study, respondents within 25-45 years old men were selected randomly among patients of LPPKN. Out of 628, only 331 respondents were selected after reviewing research protocol exclusion criteria. Each respondent was required to fill up a consent form and a GHQ-12 form. The latter was used to measure respondent's stress levels. Stress level was based on respondent's condition 3-4 weeks prior to this study. Semen sample was collected based on WHO (2010) standard semen sample collection protocol. Semen analysis, papanicoulau stain and neutral comet assay were used to measure the semen quality, percentage of sperm cytoplasm droplet abnormality and sperm DNA damage, respectively. The results showed that there was no significant association between psychostress and sperm cytoplasm droplet abnormality (U=895.50, p=0.08). Nevertheless, the percentage of normal morphology (U=6317.50, p<0.05) and DNA damage (U=1047.00, p<0.01) were affected by psychostress. As a conclusion, chronic psychostress may affect the semen quality and induced sperm DNA damage and influences the fertility eventually.

Original languageIndonesian
Pages (from-to)1931-1938
Number of pages8
JournalSains Malaysiana
Volume45
Issue number12
Publication statusPublished - 1 Dec 2016

Fingerprint

DNA damage
droplets
semen
cytoplasm
spermatozoa
sampling
DNA
assays

Keywords

  • Human sperm
  • Psycostress
  • Sperm cytoplasm droplet
  • Sperm DNA damage

ASJC Scopus subject areas

  • General

Cite this

Kesan psikostres terhadap kerosakan DNA dan ketaknormalan titisan sitoplasma sperma manusia. / Hashim, Norhamizan; Osman, Khairul; Ibrahim, Siti Fatimah; Harun, Rosliah; Mohamed, Rafeah Pakri.

In: Sains Malaysiana, Vol. 45, No. 12, 01.12.2016, p. 1931-1938.

Research output: Contribution to journalReview article

Hashim, Norhamizan ; Osman, Khairul ; Ibrahim, Siti Fatimah ; Harun, Rosliah ; Mohamed, Rafeah Pakri. / Kesan psikostres terhadap kerosakan DNA dan ketaknormalan titisan sitoplasma sperma manusia. In: Sains Malaysiana. 2016 ; Vol. 45, No. 12. pp. 1931-1938.
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