Effect of preparative variables on small interfering RNA loaded Poly(D,L-lactide-co-glycolide)-chitosan submicron particles prepared by emulsification diffusion method

Haliza Katas, Shu Chen, Adebayo Adetayo Osamuyimen, Erdal Cevher, H. Oya Alpar

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this study, poly(D,L-lactide-co-glycolide) (PLGA)-chitosan particles were investigated as an effective delivery system for small interfering RNA (siRNA) by emulsification diffusion method. The type, molecular weight and concentration of chitosan, PLGA type as well as centrifugation and freeze-drying process were amongst the investigated variables. PLGA-chitosan particles obtained were positively charged with particle size between ∼0.4-1 μm depending on type, molecular weight and concentration of chitosan as well as type of PLGA. A better siRNA loading capacity was observed when a higher degree of 'uncapped end groups' were used. The addition of trehalose has also been shown to stabilize these particles from severe aggregation induced by freeze-drying. It was found that physical properties of PLGA-chitosan particles and their siRNA binding capacity were highly influenced by certain preparation parameters. The desired positive charge of submicron size range PLGA-chitosan particles could therefore be obtained by adjusting and optimizing these preparative and formulation parameters.

Original languageEnglish
Pages (from-to)541-548
Number of pages8
JournalJournal of Microencapsulation
Volume25
Issue number8
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Emulsification
Chitosan
RNA
Small Interfering RNA
freeze drying
Freeze Drying
molecular weight
Drying
Molecular weight
Molecular Weight
Centrifugation
Trehalose
polylactic acid-polyglycolic acid copolymer
Particle Size
delivery
Agglomeration
Physical properties
physical properties
adjusting
Particle size

Keywords

  • Chitosan
  • Emulsification diffusion method
  • Gene silencing
  • Nanoparticles
  • Poly (D,L-lactide-co-glycolide)
  • siRNA

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Bioengineering
  • Organic Chemistry

Cite this

Effect of preparative variables on small interfering RNA loaded Poly(D,L-lactide-co-glycolide)-chitosan submicron particles prepared by emulsification diffusion method. / Katas, Haliza; Chen, Shu; Osamuyimen, Adebayo Adetayo; Cevher, Erdal; Alpar, H. Oya.

In: Journal of Microencapsulation, Vol. 25, No. 8, 2008, p. 541-548.

Research output: Contribution to journalArticle

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