Effect of preculture, pvs2 and vitamin c on survival of recalcitrant Nephelium ramboutan-ake shoot tips after cryopreservation by vitrification

S. P. Chua, Normah Mohd. Noor

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This paper reports the cryopreservation of Nephelium ramboutan-ake shoot tips derived from in vitro shoot multiplication and in vitro seed germination using vitrification. Preculture with either 0.5 M sucrose for 2 days or a combination of 0.3 M sucrose and 0.5 M glycerol for 3 days enhanced dehydration tolerance and resulted in the highest survival of shoot tips; however, none of the shoot tips withstood liquid nitrogen (LN) exposure. The use of a lower temperature (0°C) during exposure to plant vitrification solution (PVS2) led to higher survival of shoot tips, compared to exposure at 25°C. The survival percentage of shoot tips exposed to PVS2 for up to 20 min at 0°C was 83.3%. It was only 53.3% when shoot tips were exposed to PVS2 at 25°C for 5 min. The importance of vitamin C for reducing oxidative stress in shoots tips was demonstrated. The addition of 0.28 raM vitamin C during critical steps of the vitrification process resulted in a high survival (96.67%) without LN exposure, compared to 73.3% for shoot tips not treated with vitamin C. Moreover, 3.3% shoot tips withstood LN exposure when vitamin C was added during the loading step. This result suggests that cryopreservation is possible for this tropical, recalcitrant seeded tree species.

Original languageEnglish
Pages (from-to)506-515
Number of pages10
JournalCryo-Letters
Volume32
Issue number6
Publication statusPublished - Nov 2011

Fingerprint

Nephelium
Vitrification
Cryopreservation
vitrification
Vitamins
cryopreservation
Ascorbic Acid
vitamins
Nitrogen
Sucrose
ascorbic acid
Germination
Dehydration
Glycerol
Seeds
Oxidative Stress
liquids
nitrogen
sucrose
Temperature

Keywords

  • Cryopreservation
  • Liquid nitrogen
  • Nephelium ramboutan-ake
  • Preculture
  • Shoot tips
  • Vitamin C
  • Vitrification

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Anatomy

Cite this

Effect of preculture, pvs2 and vitamin c on survival of recalcitrant Nephelium ramboutan-ake shoot tips after cryopreservation by vitrification. / Chua, S. P.; Mohd. Noor, Normah.

In: Cryo-Letters, Vol. 32, No. 6, 11.2011, p. 506-515.

Research output: Contribution to journalArticle

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