Effect of ovariectomy and sex hormone replacement on glutathione and glutathione-related enzymes in rat hepatocarcinogenesis

Zarida Hambali, Wan Z W Ngah, Shahrom Abd Wahid, Khalid A. Kadir

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effects of ovariectomy and hormone replacement in control and carcinogen treated female rats were investigated by measuring whole blood and liver glutathione (WGSH, HGSH), glutathione S-transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GRx) and histological evaluation. Hepatocarcinogenesis was induced by diethylnitrosamine and 2-acetylaminofluorene. In control rats not receiving carcinogen, ovariectomy significantly increased the GST and GRx activities. Replacement with either estrogen or progesterone reduced the GST activities to below intact female values whereas replacement of both hormones together brought the GST activities to that of intact females. GRx activities were brought to intact female values by replacement with estrogen or progesterone, either singly or in combination. Neither ovariectomy nor sex hormone/s replacement influenced the levels of WGSH, HGSH and GPx activities. Carcinogen administration to intact rats increased all the parameters measured. Ovariectomized rats treated with carcinogen showed lower GPx and GRx activities at 2 mths. However, replacement with either progesterone or combined estrogen and progesterone increased GPx and GRx activities to original values. On the other hand GST and GPx activities in ovariectomized rats which had carcinogen treatment were lower than intact rats after 5 mths. Replacement with hormones either singly or both brought GST and GPx activities up to intact rat levels receiving carcinogen. The levels of WGSH, HGSH and GRx activities (5 mths) in carcinogen treated rats were not influenced by ovariectomy and/or hormone/s replacement. The results from this study suggested that ovariectomy reduced the severity of hepatocarcinogenesis which was restored by sex hormone/s replacement.

Original languageEnglish
Pages (from-to)30-35
Number of pages6
JournalPathology
Volume27
Issue number1
DOIs
Publication statusPublished - 1995

Fingerprint

Gonadal Steroid Hormones
Ovariectomy
Glutathione
Carcinogens
Glutathione Peroxidase
Glutathione Transferase
Enzymes
Progesterone
Hormones
Estrogens
2-Acetylaminofluorene
Diethylnitrosamine
Estrogen Replacement Therapy
Glutathione Reductase
Liver

Keywords

  • Estrogen
  • Glutathione
  • Glutathione peroxidase
  • Glutathione reductase
  • Glutathione S-transferase
  • Hepatocarcinogenesis
  • Ovariectomy
  • Progesterone

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Effect of ovariectomy and sex hormone replacement on glutathione and glutathione-related enzymes in rat hepatocarcinogenesis. / Hambali, Zarida; Ngah, Wan Z W; Abd Wahid, Shahrom; Kadir, Khalid A.

In: Pathology, Vol. 27, No. 1, 1995, p. 30-35.

Research output: Contribution to journalArticle

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