Effect of nitrogen source and carbon to nitrogen ratio on hydrogen production using C. acetobutylicum

Mohd. Sahaid Kalil, Hisham Salem Alshiyab, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the nitrogen source and its concentration. Approach: Using different nitrogen source with different concentration on bacterial productivity of hydrogen showed to affect on both bacterial productivity of hydrogen and biomass concentration. Results: Yeast extract as nitrogen source at concentration of 13 g L-1 was the best organic nitrogen source and resulted in hydrogen yield YP/S of 308 mL g-1 glucose utilized with biomass concentration of 1.1 g L -1, hydrogen yield per biomass YP/X of 280 mL g -1 L-1, biomass per substrate utilized YX/S of 0.22 and produced hydrogen in gram per gram of glucose utilized YH2/S of 0.0275. C/N of 70 enhanced the YP/S from 308-350 mL g -1 glucose utilized with biomass concentration of 1.22 gL -1, YP/X of 287 mL g-1 L-1, Y X/S of 0.244 and YH2/S of 0.03125. Conclusion: Nitrogen source with proper C:N ratio enhanced the hydrogen production.

Original languageEnglish
Pages (from-to)393-401
Number of pages9
JournalAmerican Journal of Biochemistry and Biotechnology
Volume4
Issue number4
DOIs
Publication statusPublished - 2008

Fingerprint

Hydrogen production
Hydrogen
Nitrogen
Carbon
Biomass
Productivity
Glucose
Yeast
Yeasts
Substrates

Keywords

  • Anaerobic fermentation
  • Clostridium
  • Hydrogen production
  • Yeast extract

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology

Cite this

Effect of nitrogen source and carbon to nitrogen ratio on hydrogen production using C. acetobutylicum. / Kalil, Mohd. Sahaid; Alshiyab, Hisham Salem; Wan Yusoff, Wan Mohtar.

In: American Journal of Biochemistry and Biotechnology, Vol. 4, No. 4, 2008, p. 393-401.

Research output: Contribution to journalArticle

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N2 - Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the nitrogen source and its concentration. Approach: Using different nitrogen source with different concentration on bacterial productivity of hydrogen showed to affect on both bacterial productivity of hydrogen and biomass concentration. Results: Yeast extract as nitrogen source at concentration of 13 g L-1 was the best organic nitrogen source and resulted in hydrogen yield YP/S of 308 mL g-1 glucose utilized with biomass concentration of 1.1 g L -1, hydrogen yield per biomass YP/X of 280 mL g -1 L-1, biomass per substrate utilized YX/S of 0.22 and produced hydrogen in gram per gram of glucose utilized YH2/S of 0.0275. C/N of 70 enhanced the YP/S from 308-350 mL g -1 glucose utilized with biomass concentration of 1.22 gL -1, YP/X of 287 mL g-1 L-1, Y X/S of 0.244 and YH2/S of 0.03125. Conclusion: Nitrogen source with proper C:N ratio enhanced the hydrogen production.

AB - Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the nitrogen source and its concentration. Approach: Using different nitrogen source with different concentration on bacterial productivity of hydrogen showed to affect on both bacterial productivity of hydrogen and biomass concentration. Results: Yeast extract as nitrogen source at concentration of 13 g L-1 was the best organic nitrogen source and resulted in hydrogen yield YP/S of 308 mL g-1 glucose utilized with biomass concentration of 1.1 g L -1, hydrogen yield per biomass YP/X of 280 mL g -1 L-1, biomass per substrate utilized YX/S of 0.22 and produced hydrogen in gram per gram of glucose utilized YH2/S of 0.0275. C/N of 70 enhanced the YP/S from 308-350 mL g -1 glucose utilized with biomass concentration of 1.22 gL -1, YP/X of 287 mL g-1 L-1, Y X/S of 0.244 and YH2/S of 0.03125. Conclusion: Nitrogen source with proper C:N ratio enhanced the hydrogen production.

KW - Anaerobic fermentation

KW - Clostridium

KW - Hydrogen production

KW - Yeast extract

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