Effect of nicotine on glutathione metabolizing enzymes and some conjugation enzymes in the rat

W. Z. Wan Ngah, Z. Jarien, M. H. Rajikin, N. A. Shamaan

    Research output: Contribution to journalArticle

    Abstract

    The effects of nicotine, a major component of cigarette smoke on the conjugation enzymes and glutathione metabolizing enzymes in the liver were investigated. The enzymes investigated were EDP-glucuronyl transferase (UDP-GT), sulphotransferase (ST), glutathione S-transferase (GSH-Tr), glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rx). GSH-Tr, GSH-Px and GSH-Rx were also determined in the lung. The enzymes are the usual enzymes involved in the detoxication of foreign compounds and peroxide metabolism. Two groups of 16 male rats, Rattus norwegicus weighing between 150-170g were used; the first group represents the control whereas the second group the nicotine-treated rats. The rats were killed after 20, 40, 60 and 100 days. Liver microsomal UDP-GT activities were found to be significantly higher in the nicotine-treated rats when compared to the control rats (p<0.05). There was about a two-fold increase in the enzyme activity and the increase was constant with increasing time of nicotine treatment. The liver cytosolic ST activities were also significantly increased (p<0.05) when compared to the control rats. The ST activities were increased by two to three fold and the increases were again constant over the days of treatment. Liver cytosolic GSH-Tr activities in the nicotine treated rats were found to be raised (p<0.05) as compared to the control rats and these increases were constant over the days of treatment where the increase after 100 days of treatment was the same as after 20 days of treatment. GSH-Tr activities in the lung were also determined and found to be slightly raised although the GSH-Tr activities in the lung were one-tenth that of the liver. Both GSH-Px and GSH-Rx activities were higher in the lung when compared to that of the liver. Administration of nicotine caused a significant increase in the ezyme activities in both the lungs and liver. However, the increases were again constant and remained unchanged with increasing time of administration of nicotine. The results show that the three liver conjugation enzyme activities and lung glutathione metabolizing enzyme activities were increased by the administration of nicotine.

    Original languageEnglish
    Pages (from-to)55-61
    Number of pages7
    JournalAsia Pacific Journal of Pharmacology
    Volume6
    Issue number1
    Publication statusPublished - 1991

    Fingerprint

    Nicotine
    Glutathione
    Enzymes
    Liver
    Sulfotransferases
    Lung
    Glutathione Peroxidase
    Glucuronosyltransferase
    Uridine Diphosphate
    Glutathione Reductase
    Peroxides
    Glutathione Transferase
    Smoke
    Tobacco Products
    Control Groups

    ASJC Scopus subject areas

    • Pharmacology

    Cite this

    Wan Ngah, W. Z., Jarien, Z., Rajikin, M. H., & Shamaan, N. A. (1991). Effect of nicotine on glutathione metabolizing enzymes and some conjugation enzymes in the rat. Asia Pacific Journal of Pharmacology, 6(1), 55-61.

    Effect of nicotine on glutathione metabolizing enzymes and some conjugation enzymes in the rat. / Wan Ngah, W. Z.; Jarien, Z.; Rajikin, M. H.; Shamaan, N. A.

    In: Asia Pacific Journal of Pharmacology, Vol. 6, No. 1, 1991, p. 55-61.

    Research output: Contribution to journalArticle

    Wan Ngah, WZ, Jarien, Z, Rajikin, MH & Shamaan, NA 1991, 'Effect of nicotine on glutathione metabolizing enzymes and some conjugation enzymes in the rat', Asia Pacific Journal of Pharmacology, vol. 6, no. 1, pp. 55-61.
    Wan Ngah, W. Z. ; Jarien, Z. ; Rajikin, M. H. ; Shamaan, N. A. / Effect of nicotine on glutathione metabolizing enzymes and some conjugation enzymes in the rat. In: Asia Pacific Journal of Pharmacology. 1991 ; Vol. 6, No. 1. pp. 55-61.
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    abstract = "The effects of nicotine, a major component of cigarette smoke on the conjugation enzymes and glutathione metabolizing enzymes in the liver were investigated. The enzymes investigated were EDP-glucuronyl transferase (UDP-GT), sulphotransferase (ST), glutathione S-transferase (GSH-Tr), glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rx). GSH-Tr, GSH-Px and GSH-Rx were also determined in the lung. The enzymes are the usual enzymes involved in the detoxication of foreign compounds and peroxide metabolism. Two groups of 16 male rats, Rattus norwegicus weighing between 150-170g were used; the first group represents the control whereas the second group the nicotine-treated rats. The rats were killed after 20, 40, 60 and 100 days. Liver microsomal UDP-GT activities were found to be significantly higher in the nicotine-treated rats when compared to the control rats (p<0.05). There was about a two-fold increase in the enzyme activity and the increase was constant with increasing time of nicotine treatment. The liver cytosolic ST activities were also significantly increased (p<0.05) when compared to the control rats. The ST activities were increased by two to three fold and the increases were again constant over the days of treatment. Liver cytosolic GSH-Tr activities in the nicotine treated rats were found to be raised (p<0.05) as compared to the control rats and these increases were constant over the days of treatment where the increase after 100 days of treatment was the same as after 20 days of treatment. GSH-Tr activities in the lung were also determined and found to be slightly raised although the GSH-Tr activities in the lung were one-tenth that of the liver. Both GSH-Px and GSH-Rx activities were higher in the lung when compared to that of the liver. Administration of nicotine caused a significant increase in the ezyme activities in both the lungs and liver. However, the increases were again constant and remained unchanged with increasing time of administration of nicotine. The results show that the three liver conjugation enzyme activities and lung glutathione metabolizing enzyme activities were increased by the administration of nicotine.",
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