Effect of natural honey on human platelets and blood coagulation proteins

Asif Ahmed, Rafeeq Alam Khan, M. Kamran Azim, S. Arshad Saeed, Osman Mesaik Mohammed Ahmed Hassan, Shakil Ahmed, Imran Imran

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Present study was conducted to determine the effects of honey on blood hemostasis, in-vitro effect of honey was observed on platelet aggregation and blood coagulation employing, activated partial prothrombin time (aPTT), prothrombin time (PT), thrombin time (TT) and fibrinogen levels in blood. Honey samples showed moderate inhibition of platelet aggregation with IC50 5-7.5%. The coagulation assays showed that at higher concentrations (≥15%) honey samples increased whole blood clotting time. When assayed in platelet poor plasma (PPP), honey samples significantly (P≥0.005) prolonged aPTT, PT, and TT. The honey samples (at 3.75% and 7.5% concentrations) cause mean increment of aPTT = 19±10% and 62±10%; PT 6±5% and 40±5%; TT 35±15% and 112±30% respectively. Moreover, PPP isolated from whole blood pre-incubated with honey samples (9.0% for 10 minutes) showed mean prolongation of aPTT, PT and TT of 45±21%, 26±9% and 105±24% respectively. Interestingly, incubation of honey at 6.25% and 11.75% concentrations in PPP considerably (P≥0.005) reduced fibrinogen levels i.e. 13±4% and 86±30% respectively. The present study outlines the inhibitory effect of natural honey on platelet aggregation and blood coagulation. These observations provide first line data for modulatory role(s) of honey on process of hemostasis.

Original languageEnglish
Pages (from-to)389-397
Number of pages9
JournalPakistan Journal of Pharmaceutical Sciences
Volume24
Issue number3
Publication statusPublished - Jul 2011
Externally publishedYes

Fingerprint

Honey
Blood Coagulation
Prothrombin Time
Blood Proteins
Blood Platelets
Thrombin Time
Platelet Aggregation
Hemostasis
Fibrinogen
Inhibitory Concentration 50

Keywords

  • Anti-platelet
  • Anticoagulation
  • Cardiovascular diseases and cerebro-vascular accident
  • Nutraceuticals
  • Thrombosis

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Ahmed, A., Khan, R. A., Azim, M. K., Saeed, S. A., Mohammed Ahmed Hassan, O. M., Ahmed, S., & Imran, I. (2011). Effect of natural honey on human platelets and blood coagulation proteins. Pakistan Journal of Pharmaceutical Sciences, 24(3), 389-397.

Effect of natural honey on human platelets and blood coagulation proteins. / Ahmed, Asif; Khan, Rafeeq Alam; Azim, M. Kamran; Saeed, S. Arshad; Mohammed Ahmed Hassan, Osman Mesaik; Ahmed, Shakil; Imran, Imran.

In: Pakistan Journal of Pharmaceutical Sciences, Vol. 24, No. 3, 07.2011, p. 389-397.

Research output: Contribution to journalArticle

Ahmed, A, Khan, RA, Azim, MK, Saeed, SA, Mohammed Ahmed Hassan, OM, Ahmed, S & Imran, I 2011, 'Effect of natural honey on human platelets and blood coagulation proteins', Pakistan Journal of Pharmaceutical Sciences, vol. 24, no. 3, pp. 389-397.
Ahmed A, Khan RA, Azim MK, Saeed SA, Mohammed Ahmed Hassan OM, Ahmed S et al. Effect of natural honey on human platelets and blood coagulation proteins. Pakistan Journal of Pharmaceutical Sciences. 2011 Jul;24(3):389-397.
Ahmed, Asif ; Khan, Rafeeq Alam ; Azim, M. Kamran ; Saeed, S. Arshad ; Mohammed Ahmed Hassan, Osman Mesaik ; Ahmed, Shakil ; Imran, Imran. / Effect of natural honey on human platelets and blood coagulation proteins. In: Pakistan Journal of Pharmaceutical Sciences. 2011 ; Vol. 24, No. 3. pp. 389-397.
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