Effect of methanolic extract of Piper sarmentosum leaves on neointimal foam cell infiltration in rabbits fed with high cholesterol diet

Adel A. Amran, Zaiton Zakaria, Faizah Othman, Srijit Das, Hesham M. Al-Mekhlafi, Santhana Raj, Nor Anita M M Nordin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Previous research has shown the beneficial effects of aqueous extract of Piper sarmentosum (P.s) on atherosclerosis. The first stage in atherosclerosis is the formation of foam cell. The aim of this study was to investigate the effect of the methanol extract of P.s on fatty streaks by calculating neointimal foam cell infiltration in rabbits fed with high cholesterol diet. Thirty six male New Zealand white rabbits were divided equally into six groups: (i) C: control group fed normal rabbit chow; (ii) CH: cholesterol diet (1 % cholesterol); (iii) PM1: 1 % cholesterol with methanol extract of P.s (62.5 mg/kg); (iv) PM2: 1 % cholesterol with methanol extract of P.s (125 mg/kg); (v) PM3: 1 % cholesterol with methanol extract of P.s (250 mg/kg); (vi) SMV group fed 1 % cholesterol supplemented with Simvistatin drug (1.2 mg/kg). All animals were treated for 10 weeks. At the end of the treatment, the rabbits were fasted and sacrificed and the aortic tissues were collected for histological studies to measure the area of the neointimal foam cell infiltration using software. The thickening of intima ratio of atherosclerosis and morphological changes by scanning electron microscope were measured. The results showed that the atherosclerotic group had significantly bigger area of fatty streak compared to the control group. The area of fatty streak in the abdominal aorta was significantly reduced in the treatment groups which were similar with the SMV group. Similarly, there was a reduction in the number of foam cell in the treatment groups compared to the atherosclerotic group as seen under scanning microscope. In conclusion, histological study demonstrated that the methanol extract of the P.s could reduce the neointimal foam cell infiltration in the lumen of the aorta and the atherosclerotic lesion.

Original languageEnglish
Pages (from-to)274-283
Number of pages10
JournalEXCLI Journal
Volume11
Publication statusPublished - 2012

Fingerprint

Piper
foam cells
Foam Cells
high fat diet
infiltration (hydrology)
rabbits
Cholesterol
cholesterol
Diet
Rabbits
methanol
Methanol
extracts
atherosclerosis
leaves
Atherosclerosis
aorta
New Zealand White rabbit
Control Groups
scanning electron microscopes

Keywords

  • Aorta
  • Atherosclerosis
  • Extract
  • Histology
  • Piper sarmentosum
  • Simvistatin

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Animal Science and Zoology

Cite this

Amran, A. A., Zakaria, Z., Othman, F., Das, S., Al-Mekhlafi, H. M., Raj, S., & Nordin, N. A. M. M. (2012). Effect of methanolic extract of Piper sarmentosum leaves on neointimal foam cell infiltration in rabbits fed with high cholesterol diet. EXCLI Journal, 11, 274-283.

Effect of methanolic extract of Piper sarmentosum leaves on neointimal foam cell infiltration in rabbits fed with high cholesterol diet. / Amran, Adel A.; Zakaria, Zaiton; Othman, Faizah; Das, Srijit; Al-Mekhlafi, Hesham M.; Raj, Santhana; Nordin, Nor Anita M M.

In: EXCLI Journal, Vol. 11, 2012, p. 274-283.

Research output: Contribution to journalArticle

Amran, AA, Zakaria, Z, Othman, F, Das, S, Al-Mekhlafi, HM, Raj, S & Nordin, NAMM 2012, 'Effect of methanolic extract of Piper sarmentosum leaves on neointimal foam cell infiltration in rabbits fed with high cholesterol diet', EXCLI Journal, vol. 11, pp. 274-283.
Amran, Adel A. ; Zakaria, Zaiton ; Othman, Faizah ; Das, Srijit ; Al-Mekhlafi, Hesham M. ; Raj, Santhana ; Nordin, Nor Anita M M. / Effect of methanolic extract of Piper sarmentosum leaves on neointimal foam cell infiltration in rabbits fed with high cholesterol diet. In: EXCLI Journal. 2012 ; Vol. 11. pp. 274-283.
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abstract = "Previous research has shown the beneficial effects of aqueous extract of Piper sarmentosum (P.s) on atherosclerosis. The first stage in atherosclerosis is the formation of foam cell. The aim of this study was to investigate the effect of the methanol extract of P.s on fatty streaks by calculating neointimal foam cell infiltration in rabbits fed with high cholesterol diet. Thirty six male New Zealand white rabbits were divided equally into six groups: (i) C: control group fed normal rabbit chow; (ii) CH: cholesterol diet (1 {\%} cholesterol); (iii) PM1: 1 {\%} cholesterol with methanol extract of P.s (62.5 mg/kg); (iv) PM2: 1 {\%} cholesterol with methanol extract of P.s (125 mg/kg); (v) PM3: 1 {\%} cholesterol with methanol extract of P.s (250 mg/kg); (vi) SMV group fed 1 {\%} cholesterol supplemented with Simvistatin drug (1.2 mg/kg). All animals were treated for 10 weeks. At the end of the treatment, the rabbits were fasted and sacrificed and the aortic tissues were collected for histological studies to measure the area of the neointimal foam cell infiltration using software. The thickening of intima ratio of atherosclerosis and morphological changes by scanning electron microscope were measured. The results showed that the atherosclerotic group had significantly bigger area of fatty streak compared to the control group. The area of fatty streak in the abdominal aorta was significantly reduced in the treatment groups which were similar with the SMV group. Similarly, there was a reduction in the number of foam cell in the treatment groups compared to the atherosclerotic group as seen under scanning microscope. In conclusion, histological study demonstrated that the methanol extract of the P.s could reduce the neointimal foam cell infiltration in the lumen of the aorta and the atherosclerotic lesion.",
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