Effect of Marantodes pumilum Blume (Kuntze) var.alata on β-cell function and insulin signaling in ovariectomised diabetic rats

M. Dharmani, K. Kamarulzaman, N. Giribabu, K. W. Choy, M. Z. Zuhaida, N. A. Aladdin, J. A. Jamal, M. R. Mustafa

Research output: Contribution to journalArticle

Abstract

Background: Oestrogen deficiency leads to metabolic disturbances such as insulin resistance and impairment of adipose tissue or lipid metabolism. Marantodes pumilum (Blume) Kuntze (Primulaceae) is believed to have phytoestrogenic properties and is claimed to have beneficial effects in the treatment of diabetes mellitus (DM), but the mechanism behind its phytoestrogenic effects on estrogen-deficient diabetic condition have not been fully examined. Purpose: The present study investigated the effects of oral treatment with M. pumilum var. alata (MPA) extracts on the estrogen receptor, metabolic characteristics and insulin signaling pathway in pancreas and liver of ovariectomised nicotidamide streptozotocin-induced diabetes in female rats. Materials and methods: Ovariectomised diabetic (OVXS) Sprague-Dawley rats were orally administered with either aqueous leaf extract and ethanol (50%) stem-root extract of MPA (50 or 100 mg/kg) respectively for 28 days. Metabolic parameters were evaluated by measuring fasting blood glucose, serum insulin, oral glucose and insulin tolerance test. Distribution and expression level of insulin, oxidative stress and inflammatory marker in the pancreatic islets and liver were evaluated by immunohistochemistry and western blot, respectively. Results: Oral treatment with aqueous leaf and ethanol (50%) stem-root extracts of MPA (100 mg/kg) significantly reversed the elevated fasting blood glucose, impaired glucose and insulin tolerance. The protein expression of insulin, glucose transporter (GLUT-2 and GLUT-4) increased in the pancreatic islets and liver. Furthermore, marked improvement in the tissue morphology following treatment with MPA was observed. Similarly, the western blots analysis denotes improved insulin signaling in the liver and decreased reactive oxygen species producing enzymes, inflammatory and pro-apoptotic molecules with MPA treatment. Conclusions: Taken together, this work demonstrate that 100 mg/kg of aqueous leaf extract and ethanol (50%) stem-root extract of MPA improves β-cell function and insulin signaling in postmenopausal diabetes through attenuation of oxidative stress and partially mediated by oestrogen receptor stimulation.

Original languageEnglish
Article number153101
JournalPhytomedicine
Volume65
DOIs
Publication statusPublished - Dec 2019

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Insulin
Ethanol
Liver
Islets of Langerhans
Estrogen Receptors
Blood Glucose
Primulaceae
Fasting
Estrogens
Oxidative Stress
Western Blotting
Therapeutics
Glucose Intolerance
Experimental Diabetes Mellitus
Facilitative Glucose Transport Proteins
Glucose Tolerance Test
Lipid Metabolism
Sprague Dawley Rats
Insulin Resistance
Adipose Tissue

Keywords

  • Diabetes
  • Marantodes pumilum var. alata
  • Ovariectomised
  • Oxidative stress
  • Phytoestrogen

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Dharmani, M., Kamarulzaman, K., Giribabu, N., Choy, K. W., Zuhaida, M. Z., Aladdin, N. A., ... Mustafa, M. R. (2019). Effect of Marantodes pumilum Blume (Kuntze) var.alata on β-cell function and insulin signaling in ovariectomised diabetic rats. Phytomedicine, 65, [153101]. https://doi.org/10.1016/j.phymed.2019.153101

Effect of Marantodes pumilum Blume (Kuntze) var.alata on β-cell function and insulin signaling in ovariectomised diabetic rats. / Dharmani, M.; Kamarulzaman, K.; Giribabu, N.; Choy, K. W.; Zuhaida, M. Z.; Aladdin, N. A.; Jamal, J. A.; Mustafa, M. R.

In: Phytomedicine, Vol. 65, 153101, 12.2019.

Research output: Contribution to journalArticle

Dharmani, M. ; Kamarulzaman, K. ; Giribabu, N. ; Choy, K. W. ; Zuhaida, M. Z. ; Aladdin, N. A. ; Jamal, J. A. ; Mustafa, M. R. / Effect of Marantodes pumilum Blume (Kuntze) var.alata on β-cell function and insulin signaling in ovariectomised diabetic rats. In: Phytomedicine. 2019 ; Vol. 65.
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abstract = "Background: Oestrogen deficiency leads to metabolic disturbances such as insulin resistance and impairment of adipose tissue or lipid metabolism. Marantodes pumilum (Blume) Kuntze (Primulaceae) is believed to have phytoestrogenic properties and is claimed to have beneficial effects in the treatment of diabetes mellitus (DM), but the mechanism behind its phytoestrogenic effects on estrogen-deficient diabetic condition have not been fully examined. Purpose: The present study investigated the effects of oral treatment with M. pumilum var. alata (MPA) extracts on the estrogen receptor, metabolic characteristics and insulin signaling pathway in pancreas and liver of ovariectomised nicotidamide streptozotocin-induced diabetes in female rats. Materials and methods: Ovariectomised diabetic (OVXS) Sprague-Dawley rats were orally administered with either aqueous leaf extract and ethanol (50{\%}) stem-root extract of MPA (50 or 100 mg/kg) respectively for 28 days. Metabolic parameters were evaluated by measuring fasting blood glucose, serum insulin, oral glucose and insulin tolerance test. Distribution and expression level of insulin, oxidative stress and inflammatory marker in the pancreatic islets and liver were evaluated by immunohistochemistry and western blot, respectively. Results: Oral treatment with aqueous leaf and ethanol (50{\%}) stem-root extracts of MPA (100 mg/kg) significantly reversed the elevated fasting blood glucose, impaired glucose and insulin tolerance. The protein expression of insulin, glucose transporter (GLUT-2 and GLUT-4) increased in the pancreatic islets and liver. Furthermore, marked improvement in the tissue morphology following treatment with MPA was observed. Similarly, the western blots analysis denotes improved insulin signaling in the liver and decreased reactive oxygen species producing enzymes, inflammatory and pro-apoptotic molecules with MPA treatment. Conclusions: Taken together, this work demonstrate that 100 mg/kg of aqueous leaf extract and ethanol (50{\%}) stem-root extract of MPA improves β-cell function and insulin signaling in postmenopausal diabetes through attenuation of oxidative stress and partially mediated by oestrogen receptor stimulation.",
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AU - Giribabu, N.

AU - Choy, K. W.

AU - Zuhaida, M. Z.

AU - Aladdin, N. A.

AU - Jamal, J. A.

AU - Mustafa, M. R.

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AB - Background: Oestrogen deficiency leads to metabolic disturbances such as insulin resistance and impairment of adipose tissue or lipid metabolism. Marantodes pumilum (Blume) Kuntze (Primulaceae) is believed to have phytoestrogenic properties and is claimed to have beneficial effects in the treatment of diabetes mellitus (DM), but the mechanism behind its phytoestrogenic effects on estrogen-deficient diabetic condition have not been fully examined. Purpose: The present study investigated the effects of oral treatment with M. pumilum var. alata (MPA) extracts on the estrogen receptor, metabolic characteristics and insulin signaling pathway in pancreas and liver of ovariectomised nicotidamide streptozotocin-induced diabetes in female rats. Materials and methods: Ovariectomised diabetic (OVXS) Sprague-Dawley rats were orally administered with either aqueous leaf extract and ethanol (50%) stem-root extract of MPA (50 or 100 mg/kg) respectively for 28 days. Metabolic parameters were evaluated by measuring fasting blood glucose, serum insulin, oral glucose and insulin tolerance test. Distribution and expression level of insulin, oxidative stress and inflammatory marker in the pancreatic islets and liver were evaluated by immunohistochemistry and western blot, respectively. Results: Oral treatment with aqueous leaf and ethanol (50%) stem-root extracts of MPA (100 mg/kg) significantly reversed the elevated fasting blood glucose, impaired glucose and insulin tolerance. The protein expression of insulin, glucose transporter (GLUT-2 and GLUT-4) increased in the pancreatic islets and liver. Furthermore, marked improvement in the tissue morphology following treatment with MPA was observed. Similarly, the western blots analysis denotes improved insulin signaling in the liver and decreased reactive oxygen species producing enzymes, inflammatory and pro-apoptotic molecules with MPA treatment. Conclusions: Taken together, this work demonstrate that 100 mg/kg of aqueous leaf extract and ethanol (50%) stem-root extract of MPA improves β-cell function and insulin signaling in postmenopausal diabetes through attenuation of oxidative stress and partially mediated by oestrogen receptor stimulation.

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KW - Oxidative stress

KW - Phytoestrogen

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