Effect of ling zhi or reishi mushroom Ganoderma lucidum (W. Curt.

Fr.) P. Karst. mycelium on benzo[a]prene-induced early alterations of the respiratory epithelium in the hypercholesterolaemic rats

Yew Keong Choong, Noordin M. Mustapha, Suhaila Mohamed, Nor Aini B Umar, Chow Chin Tong

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Many research studies indicate that fractions from the culture medium of Ganoderma lucidum mycelium (GM) have an antitumor activity through immunostimulation. There is a growing need for a short-term, cost-effective bioassay to assess the efficacy of potential antitumor agents from GM. Twenty-five Spraque-Dawley rats were divided into 5 groups according to their different feed. The positive control and negative control groups were given basal rat chew. The cholesterol (Chol) group was fed a 1% cholesterol diet, and the GM group was fed a 10% GM diet, whereby 1% Chol and 10% GM powder was added to the cholesterol plus G. lucidum mycelium (Chol+GM) group diet, respectively. All the rats were sacrificed 3 months after instillation with 13 ng of benzo[a]prene (B(a)P), except the negative control group. The lung sections were preserved for histological examination, and the glutathione S-transferase (GST) level was measured. Supplementation with GM had no significant effect on the increasing ratio of lung weight to body weight of the rats. The level of GST was significantly lower in both the Chol and positive control groups compared to the other groups. In the Chol+GM group, no significant lesions were observed in the lungs. No significant gross pathological changes were observed in the lungs of the negative control, GM, or Chol+GM groups. However, small whitish nodules were observed in the lungs of 3 rats from the positive control group and on both lungs in all rats from the Chol group. The study reported herein showed that GM is an effective treatment in inhibiting B(a)P-induced carcinogenesis in rats.

    Original languageEnglish
    Pages (from-to)139-150
    Number of pages12
    JournalInternational Journal of Medicinal Mushrooms
    Volume9
    Issue number2
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Reishi
    respiratory mucosa
    Ganoderma lucidum
    Respiratory Mucosa
    Agaricales
    Mycelium
    karsts
    mushrooms
    mycelium
    Cholesterol
    rats
    cholesterol
    lungs
    Lung
    Control Groups
    Diet
    Glutathione Transferase
    glutathione transferase
    diet
    antineoplastic agents

    Keywords

    • Benzo[a]prene
    • Cholesterol
    • Ganoderma lucidum mycelium
    • Hypercholesterolaemia
    • Respiratory epithelium

    ASJC Scopus subject areas

    • Molecular Medicine
    • Plant Science
    • Pharmacology, Toxicology and Pharmaceutics(all)

    Cite this

    Effect of ling zhi or reishi mushroom Ganoderma lucidum (W. Curt. Fr.) P. Karst. mycelium on benzo[a]prene-induced early alterations of the respiratory epithelium in the hypercholesterolaemic rats. / Choong, Yew Keong; Mustapha, Noordin M.; Mohamed, Suhaila; Umar, Nor Aini B; Tong, Chow Chin.

    In: International Journal of Medicinal Mushrooms, Vol. 9, No. 2, 2007, p. 139-150.

    Research output: Contribution to journalArticle

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    abstract = "Many research studies indicate that fractions from the culture medium of Ganoderma lucidum mycelium (GM) have an antitumor activity through immunostimulation. There is a growing need for a short-term, cost-effective bioassay to assess the efficacy of potential antitumor agents from GM. Twenty-five Spraque-Dawley rats were divided into 5 groups according to their different feed. The positive control and negative control groups were given basal rat chew. The cholesterol (Chol) group was fed a 1{\%} cholesterol diet, and the GM group was fed a 10{\%} GM diet, whereby 1{\%} Chol and 10{\%} GM powder was added to the cholesterol plus G. lucidum mycelium (Chol+GM) group diet, respectively. All the rats were sacrificed 3 months after instillation with 13 ng of benzo[a]prene (B(a)P), except the negative control group. The lung sections were preserved for histological examination, and the glutathione S-transferase (GST) level was measured. Supplementation with GM had no significant effect on the increasing ratio of lung weight to body weight of the rats. The level of GST was significantly lower in both the Chol and positive control groups compared to the other groups. In the Chol+GM group, no significant lesions were observed in the lungs. No significant gross pathological changes were observed in the lungs of the negative control, GM, or Chol+GM groups. However, small whitish nodules were observed in the lungs of 3 rats from the positive control group and on both lungs in all rats from the Chol group. The study reported herein showed that GM is an effective treatment in inhibiting B(a)P-induced carcinogenesis in rats.",
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