Effect of irradiation and polyethylene glycol on drought tolerance of MR269 genotype rice (Oryza sativa L.)

Ahsan A. Kadhimi, Che Radziah Che Mohd. Zain, Arshad Naji Alhasnawi, Anizan Isahak, Mehdi Farshad Ashraf, Azhar Mohamad, Febri Doni, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: A drought-tolerant genotype should be identified and improved for the continuity of food security because of the global importance of rice. Mutation and tissue culture techniques are effective and important for studies on the development of potential rice resources. The effects of irradiation and drought stress on rice genotype MR269 of Oryza sativa L., differing in drought tolerance capability were investigated. Methodology: The seeds of the genotypes were exposed to gamma rays at 350 Gy. Naked seeds were initiated onto half-strength standard MS medium enhanced with 0, 10 and 20% polyethylene glycol (PEG) 6000. The effects of PEG on root and shoot length, fresh and dry seedling weights, total chlorophyll and carbohydrate contents and proline accumulation were examined. Significant differences were observed in the studied traits. The non-irradiated seeds presented the highest mean shoot and seedling fresh weight, whereas the irradiated seeds presented the largest root length and highest proline concentrations. Results: The PEG produced a decrease in the means of all the characteristics investigated by enhancing PEG concentration, except for the means of proline content, which increased with the increase in PEG concentration. The irradiated genotypes produced the highest values of root length, dry weight, chlorophyll, proline and carbohydrates in concentrations of 20% PEG compared with the non-irradiated ones. Conclusion: Thus, a positive signal exists in the effect of radiation on these characteristics in drought tolerance. Research on the improvement of a drought-tolerant genotype under different drought conditions is necessary to enhance rice productivity and food security.

Original languageEnglish
Pages (from-to)52-59
Number of pages8
JournalAsian Journal of Crop Science
Volume8
Issue number2
DOIs
Publication statusPublished - 2016

Fingerprint

polyethylene glycol
drought tolerance
Oryza sativa
irradiation
genotype
rice
tolerance
drought
proline
seed
food security
carbohydrate
seeds
chlorophyll
shoot
seedling
drought stress
shoots
seedlings
mutation

Keywords

  • Drought tolerance
  • Irradiation
  • Proline
  • Rice MR269

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science

Cite this

Effect of irradiation and polyethylene glycol on drought tolerance of MR269 genotype rice (Oryza sativa L.). / Kadhimi, Ahsan A.; Che Mohd. Zain, Che Radziah; Alhasnawi, Arshad Naji; Isahak, Anizan; Ashraf, Mehdi Farshad; Mohamad, Azhar; Doni, Febri; Wan Yusoff, Wan Mohtar.

In: Asian Journal of Crop Science, Vol. 8, No. 2, 2016, p. 52-59.

Research output: Contribution to journalArticle

Kadhimi, Ahsan A. ; Che Mohd. Zain, Che Radziah ; Alhasnawi, Arshad Naji ; Isahak, Anizan ; Ashraf, Mehdi Farshad ; Mohamad, Azhar ; Doni, Febri ; Wan Yusoff, Wan Mohtar. / Effect of irradiation and polyethylene glycol on drought tolerance of MR269 genotype rice (Oryza sativa L.). In: Asian Journal of Crop Science. 2016 ; Vol. 8, No. 2. pp. 52-59.
@article{0ad0126eb4ad43ea8204ec757897dea8,
title = "Effect of irradiation and polyethylene glycol on drought tolerance of MR269 genotype rice (Oryza sativa L.)",
abstract = "Background: A drought-tolerant genotype should be identified and improved for the continuity of food security because of the global importance of rice. Mutation and tissue culture techniques are effective and important for studies on the development of potential rice resources. The effects of irradiation and drought stress on rice genotype MR269 of Oryza sativa L., differing in drought tolerance capability were investigated. Methodology: The seeds of the genotypes were exposed to gamma rays at 350 Gy. Naked seeds were initiated onto half-strength standard MS medium enhanced with 0, 10 and 20{\%} polyethylene glycol (PEG) 6000. The effects of PEG on root and shoot length, fresh and dry seedling weights, total chlorophyll and carbohydrate contents and proline accumulation were examined. Significant differences were observed in the studied traits. The non-irradiated seeds presented the highest mean shoot and seedling fresh weight, whereas the irradiated seeds presented the largest root length and highest proline concentrations. Results: The PEG produced a decrease in the means of all the characteristics investigated by enhancing PEG concentration, except for the means of proline content, which increased with the increase in PEG concentration. The irradiated genotypes produced the highest values of root length, dry weight, chlorophyll, proline and carbohydrates in concentrations of 20{\%} PEG compared with the non-irradiated ones. Conclusion: Thus, a positive signal exists in the effect of radiation on these characteristics in drought tolerance. Research on the improvement of a drought-tolerant genotype under different drought conditions is necessary to enhance rice productivity and food security.",
keywords = "Drought tolerance, Irradiation, Proline, Rice MR269",
author = "Kadhimi, {Ahsan A.} and {Che Mohd. Zain}, {Che Radziah} and Alhasnawi, {Arshad Naji} and Anizan Isahak and Ashraf, {Mehdi Farshad} and Azhar Mohamad and Febri Doni and {Wan Yusoff}, {Wan Mohtar}",
year = "2016",
doi = "10.3923/ajcs.2016.52.59",
language = "English",
volume = "8",
pages = "52--59",
journal = "Asian Journal of Crop Science",
issn = "1994-7879",
publisher = "Asian Network for Scientific Information",
number = "2",

}

TY - JOUR

T1 - Effect of irradiation and polyethylene glycol on drought tolerance of MR269 genotype rice (Oryza sativa L.)

AU - Kadhimi, Ahsan A.

AU - Che Mohd. Zain, Che Radziah

AU - Alhasnawi, Arshad Naji

AU - Isahak, Anizan

AU - Ashraf, Mehdi Farshad

AU - Mohamad, Azhar

AU - Doni, Febri

AU - Wan Yusoff, Wan Mohtar

PY - 2016

Y1 - 2016

N2 - Background: A drought-tolerant genotype should be identified and improved for the continuity of food security because of the global importance of rice. Mutation and tissue culture techniques are effective and important for studies on the development of potential rice resources. The effects of irradiation and drought stress on rice genotype MR269 of Oryza sativa L., differing in drought tolerance capability were investigated. Methodology: The seeds of the genotypes were exposed to gamma rays at 350 Gy. Naked seeds were initiated onto half-strength standard MS medium enhanced with 0, 10 and 20% polyethylene glycol (PEG) 6000. The effects of PEG on root and shoot length, fresh and dry seedling weights, total chlorophyll and carbohydrate contents and proline accumulation were examined. Significant differences were observed in the studied traits. The non-irradiated seeds presented the highest mean shoot and seedling fresh weight, whereas the irradiated seeds presented the largest root length and highest proline concentrations. Results: The PEG produced a decrease in the means of all the characteristics investigated by enhancing PEG concentration, except for the means of proline content, which increased with the increase in PEG concentration. The irradiated genotypes produced the highest values of root length, dry weight, chlorophyll, proline and carbohydrates in concentrations of 20% PEG compared with the non-irradiated ones. Conclusion: Thus, a positive signal exists in the effect of radiation on these characteristics in drought tolerance. Research on the improvement of a drought-tolerant genotype under different drought conditions is necessary to enhance rice productivity and food security.

AB - Background: A drought-tolerant genotype should be identified and improved for the continuity of food security because of the global importance of rice. Mutation and tissue culture techniques are effective and important for studies on the development of potential rice resources. The effects of irradiation and drought stress on rice genotype MR269 of Oryza sativa L., differing in drought tolerance capability were investigated. Methodology: The seeds of the genotypes were exposed to gamma rays at 350 Gy. Naked seeds were initiated onto half-strength standard MS medium enhanced with 0, 10 and 20% polyethylene glycol (PEG) 6000. The effects of PEG on root and shoot length, fresh and dry seedling weights, total chlorophyll and carbohydrate contents and proline accumulation were examined. Significant differences were observed in the studied traits. The non-irradiated seeds presented the highest mean shoot and seedling fresh weight, whereas the irradiated seeds presented the largest root length and highest proline concentrations. Results: The PEG produced a decrease in the means of all the characteristics investigated by enhancing PEG concentration, except for the means of proline content, which increased with the increase in PEG concentration. The irradiated genotypes produced the highest values of root length, dry weight, chlorophyll, proline and carbohydrates in concentrations of 20% PEG compared with the non-irradiated ones. Conclusion: Thus, a positive signal exists in the effect of radiation on these characteristics in drought tolerance. Research on the improvement of a drought-tolerant genotype under different drought conditions is necessary to enhance rice productivity and food security.

KW - Drought tolerance

KW - Irradiation

KW - Proline

KW - Rice MR269

UR - http://www.scopus.com/inward/record.url?scp=84978050020&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978050020&partnerID=8YFLogxK

U2 - 10.3923/ajcs.2016.52.59

DO - 10.3923/ajcs.2016.52.59

M3 - Article

AN - SCOPUS:84978050020

VL - 8

SP - 52

EP - 59

JO - Asian Journal of Crop Science

JF - Asian Journal of Crop Science

SN - 1994-7879

IS - 2

ER -