Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. CV. MR219

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study, factors such as illumination, casein hydrolysate and proline were shown to produce high numbers of calli from MR219 mature seeds. The effect of different concentrations (1, 2, 3, 4 and 5 mgL-1) of 2,4-dicholorophenoxy acetic acid (2,4-D) under different illuminations (24 hours light, 16/8 hours photoperiod, dark) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (100%) and growth (90%) was observed in the Murashige and Skoog (MS) medium supplemented with 5 mgL-1 2,4-D incubated under 16/8 hours photoperiod. The effect of different concentrations of casein hydrolysate (0, 300, 400, 500, 600, 700, 80 and 1000 mgL-1) and proline (0, 500, 1000 and 1500 mgL-1) on callus induction and growth of MR219 were also studied. It was found that increasing concentrations of casein hydrolysate and proline did not give a significant effect on the percentage of callus induction. However, a significant percentage of callus growth (90%) was recorded when the callus induction medium was supplemented with 1000 mgL-1 proline.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalMalaysian Applied Biology
Volume41
Issue number1
Publication statusPublished - Jun 2012

Fingerprint

casein hydrolysates
Bony Callus
Lighting
Proline
lighting
proline
Oryza sativa
callus
2,4-Dichlorophenoxyacetic Acid
Photoperiod
Growth
2,4-D
photoperiod
Oryza
casein hydrolysate
Acetic Acid
acetic acid
Seeds
Light
seeds

Keywords

  • 2,4-dicholorophenoxy acetic acid (2,4-D)
  • Callus induction
  • Casein hydrolysate
  • Illumination
  • Proline
  • Rice (Oryza sativa L.) cv. MR219

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

@article{62f3c3d495974bb082a66067cd3e0bce,
title = "Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. CV. MR219",
abstract = "In this study, factors such as illumination, casein hydrolysate and proline were shown to produce high numbers of calli from MR219 mature seeds. The effect of different concentrations (1, 2, 3, 4 and 5 mgL-1) of 2,4-dicholorophenoxy acetic acid (2,4-D) under different illuminations (24 hours light, 16/8 hours photoperiod, dark) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (100{\%}) and growth (90{\%}) was observed in the Murashige and Skoog (MS) medium supplemented with 5 mgL-1 2,4-D incubated under 16/8 hours photoperiod. The effect of different concentrations of casein hydrolysate (0, 300, 400, 500, 600, 700, 80 and 1000 mgL-1) and proline (0, 500, 1000 and 1500 mgL-1) on callus induction and growth of MR219 were also studied. It was found that increasing concentrations of casein hydrolysate and proline did not give a significant effect on the percentage of callus induction. However, a significant percentage of callus growth (90{\%}) was recorded when the callus induction medium was supplemented with 1000 mgL-1 proline.",
keywords = "2,4-dicholorophenoxy acetic acid (2,4-D), Callus induction, Casein hydrolysate, Illumination, Proline, Rice (Oryza sativa L.) cv. MR219",
author = "{Che Mohd. Zain}, {Che Radziah} and {Siti Nurkhalida}, {A. K.} and Zamri Zainal and Ismanizan Ismail",
year = "2012",
month = "6",
language = "English",
volume = "41",
pages = "37--41",
journal = "Malaysian Applied Biology",
issn = "0126-8643",
publisher = "Malaysian Society of Applied Biology",
number = "1",

}

TY - JOUR

T1 - Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. CV. MR219

AU - Che Mohd. Zain, Che Radziah

AU - Siti Nurkhalida, A. K.

AU - Zainal, Zamri

AU - Ismail, Ismanizan

PY - 2012/6

Y1 - 2012/6

N2 - In this study, factors such as illumination, casein hydrolysate and proline were shown to produce high numbers of calli from MR219 mature seeds. The effect of different concentrations (1, 2, 3, 4 and 5 mgL-1) of 2,4-dicholorophenoxy acetic acid (2,4-D) under different illuminations (24 hours light, 16/8 hours photoperiod, dark) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (100%) and growth (90%) was observed in the Murashige and Skoog (MS) medium supplemented with 5 mgL-1 2,4-D incubated under 16/8 hours photoperiod. The effect of different concentrations of casein hydrolysate (0, 300, 400, 500, 600, 700, 80 and 1000 mgL-1) and proline (0, 500, 1000 and 1500 mgL-1) on callus induction and growth of MR219 were also studied. It was found that increasing concentrations of casein hydrolysate and proline did not give a significant effect on the percentage of callus induction. However, a significant percentage of callus growth (90%) was recorded when the callus induction medium was supplemented with 1000 mgL-1 proline.

AB - In this study, factors such as illumination, casein hydrolysate and proline were shown to produce high numbers of calli from MR219 mature seeds. The effect of different concentrations (1, 2, 3, 4 and 5 mgL-1) of 2,4-dicholorophenoxy acetic acid (2,4-D) under different illuminations (24 hours light, 16/8 hours photoperiod, dark) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (100%) and growth (90%) was observed in the Murashige and Skoog (MS) medium supplemented with 5 mgL-1 2,4-D incubated under 16/8 hours photoperiod. The effect of different concentrations of casein hydrolysate (0, 300, 400, 500, 600, 700, 80 and 1000 mgL-1) and proline (0, 500, 1000 and 1500 mgL-1) on callus induction and growth of MR219 were also studied. It was found that increasing concentrations of casein hydrolysate and proline did not give a significant effect on the percentage of callus induction. However, a significant percentage of callus growth (90%) was recorded when the callus induction medium was supplemented with 1000 mgL-1 proline.

KW - 2,4-dicholorophenoxy acetic acid (2,4-D)

KW - Callus induction

KW - Casein hydrolysate

KW - Illumination

KW - Proline

KW - Rice (Oryza sativa L.) cv. MR219

UR - http://www.scopus.com/inward/record.url?scp=84871351891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871351891&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84871351891

VL - 41

SP - 37

EP - 41

JO - Malaysian Applied Biology

JF - Malaysian Applied Biology

SN - 0126-8643

IS - 1

ER -